ANTIOXIDANT ACTIVITIES OF SILK SERICIN FROM SILKWORM<i>BOMBYX MORI</i>

Fan, Jinbo; Wu, Liping; Chen, Lishui; Mao, Xueying; Ren, Fazheng

doi:10.1111/j.1745-4514.2008.00204.x

Cited by 97 publications

(61 citation statements)

References 42 publications

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“…Sericin was indicated to suppress lipid peroxidation and tyrosinase inhibitory activity (Kato et al, 1998). In addition to antioxidant activity and chemopreventive effects of sericin was found (Fan et al, 2009;Zhaorigetu et al, 2003), Silk sericin from silkworm cocoons accelerates the proliferation of mammalian cell line (Terada et al, 2002;Aramwit et al, 2009;Aramwit and Sangcakul 2007). Another study reported anti-cancer effects of sericin in colon cancer (Kaewkom et al, 2012).…”

Section: Cytotoxicity Assay Of Cocoon Extractsmentioning

confidence: 99%

Cytocompatibility of silkworm cocoon layer extracts

Jo¹,

Kim²,

Lee³

et al. 2016

International Journal of Industrial Entomology

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Recently silk polymer produced by Bombyx mori silkworm has been considered as biological macromolecules. Silk polymer was extracted in PBS solution at 37 o C for 72 h or 72 o C for 24 h. The effect of EtOH treatment on the cocoon extraction was also examined. The extraction yield of cocoon was less than 1 wt% regardless of extraction conditions. UV spectroscopy showed that the experimental extracts have absorption bands at 280 nm. There is no cytotoxicity effect on the mouse fibroblast L929 cell. The phenotype of L929 cell was not changed under the experimental conditions. The proliferation behavior of L929 cell was not affected by the addition of cocoon extract. Therefore, cocoon extract might be cytocompatible and can be used as promising biomaterials. IntroductionA silkworm cocoon of Bombyx mori provides silk fiber for textile industries for more than 4,000 years. Recently, silk polymer has been revealed several advantages for biological materials and then applied various functional materials such as cosmetics, suture and artificial eardrum (Kundu et al., 2013;Ju et al., 2014;Vepari and Kaplan, 2007; Kim et al., 2010), and so on.In the view point of material, silk is a natural protein polymer composed of two proteins: one is a central protein called silk fibroin and another is gum-like protein called silk sericin. Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al., 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al., 2005;Meinel et al., 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.Silk sericin is a protein polymer, which acts as an adhesive binder to maintain the structure of the fibers. Sericin is an albuminoid protein composed of serine (30%) and other amino acids (Teramoto and Miyazawa, 2005;Garel et al., 1997). Sericin was removed easily from Bombyx mori cocoon using a thermo or chemical process called degumming (Mo et al., 2009) Preparation of cocoon extractsEach cocoon was washed with tap water and then dried absolutely with room temperature in the dark. The cocoon was sliced into 5 mm ⅹ 5 mm pieces. Each cocoon slices were divided into two groups; one group was treated with absolute ethyl alcohol (EtOH) for 24 h at R.T. and another group was not treated EtOH. After treatment, cocoon was dried with R.T. for overnight. The extraction condition were followed; weigh 1 g of cocoon and add into 10 mL of 1X PBS buffer at 37 o C for 72 h and/or 72 o C for 24 h. Save the cocoon extracts and then filtered it with 0.22 um syringe filter (Sartorius Ltd., Epsom, UK) and protein concentration of all extracts was determined by bicinchronic acid (BCA) assay. Forty ug of each extracts used for cell cytotoxicity and proliferation assay. UV vis sp...

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Section: Cytotoxicity Assay Of Cocoon Extractsmentioning

confidence: 99%

Cytocompatibility of silkworm cocoon layer extracts

Jo¹,

Kim²,

Lee³

et al. 2016

International Journal of Industrial Entomology

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show abstract

“…Wu et al (2007) reported that the 50% scavenging activity by DPPH assay of sericin prepared from silk industry wastewater was 31 mg/mL. Fan et al (2009) have also reported that silk sericin does not show only DPPH radical scavenging activity, but also many antioxidative activities, such as hydroxyl and superoxide radical scavenging, lipid peroxidation of linoleic acid, reducing power, and ferrous ion chelating activity.…”

Section: Free Radical Scavenging Activitymentioning

confidence: 99%

“…Silk contains fibroin, sericin, waxy components, hydrocarbons, pigments, and inorganic substances, which have nutritional value to human skin, such as carotene and linoleic acid (Kotake-Nara et al, 2002). Silk sericin is used as an improving reagent or a coating material for natural and artificial fibers, fabrics, and other articles (Fan et al, 2009). Moreover, it is also a potent natural antioxidant with high reducing power and ferrous ion chelating ability (Fan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Silk sericin is used as an improving reagent or a coating material for natural and artificial fibers, fabrics, and other articles (Fan et al, 2009). Moreover, it is also a potent natural antioxidant with high reducing power and ferrous ion chelating ability (Fan et al, 2009). The three types of silkworm [Bombyx mori (Bombycidae)] in Thailand are the Thai native, Thai hybrid (e.g., Yellow Korat,Jul No.4,and Jim Thompson), and the foreign hybrid (from Japan and China).…”

Section: Introductionmentioning

confidence: 99%

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Free radical scavenging and tyrosinase inhibition activity of oils and sericin extracted from Thai native silkworms (Bombyx mori)

Manosroi

Boonpisuttinant

Winitchai

et al. 2010

Pharmaceutical Biology

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“…In addition, various silk proteins have been studied and industrialized as a functional food due to its useful biological activities. It was reported that the Bombyx mori (B. mori) silkworm powders showed an excellent activity of lowering blood glucose levels (Ryu et al, 2002) and silk sericin exhibited a strong antioxidant activity (Fan et al, 2009). Hong et al (2002) investigated the effect of the acid hydrolysate of silk fibroin on obesity using obese mice and found that the body weight was significantly reduced in mice fed with B. mori silk fibroin hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder

Um¹,

Rico²,

Kweon³

et al. 2011

International Journal of Industrial Entomology

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The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels, and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.

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ANTIOXIDANT ACTIVITIES OF SILK SERICIN FROM SILKWORMBOMBYX MORI

Cited by 97 publications

References 42 publications

Cytocompatibility of silkworm cocoon layer extracts

Cytocompatibility of silkworm cocoon layer extracts

Free radical scavenging and tyrosinase inhibition activity of oils and sericin extracted from Thai native silkworms (Bombyx mori)

Enhancement of Lipid Metabolism and Antioxidant Defense Status in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder

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