Antioxidant Activities of Some Vegetables in Experimental Mices

Abstract

This study was carried out to evaluate the antioxidant (anticarcinogenic) effect of some vegetables (celery, green pepper and broccoli) to prevent the risk of precrcinogenic diets (10% oil used several times for fring) in the experimental animals. The results showed that the animals treated with vegtables exerted a positive effect on daily body weight, food conversion ratio, triglyceride, low density lipoprotein cholesterol (LDLc,) cholesterol, and total lipid. The inhibitory effect of vegetables on liver and renal function which was demonstrated by decreased concentrations of aspartate amino transferase (AST), (ALT), uric acid and creatinine. It could be also shown from results that vegetables diet caused significant effect in some blood parameters and blood picture. Also, laboratory analysis had beneficial effect on relative weight of liver, spleen, kidenys and brain. The experimental rats showed different pathological changes varied from mild to sever inflammation and adenoma in different organs of spleen, brain, liver, and kidneys. From this study, it is concluded that, consumption of vegetables can lower the risk of very fried oil. such as ascorbic acid (Vitamin C), urate and glutathione as well as lipophilic radical scavengers such as tocopherol (Vitamin E), carotenoids and flavonoids. Therefore, vegetables could conceivably protect against the molecular effects of lipid peroxidation, free radicals and ROS and also delay the progress of many chronic diseases (Lai et al., 2001 and Gulacin et al., 2003). The present study was designed to determine some chemical composition in some vegetables (green pepper, celery and broccoli) as antioxidant sources. We focused our attention on its effects on the serum lipid pattern parameters, liver function, kidneys function, test of some blood indicators and enzymes, as well as the histopathological examination of liver, kidneys, spleen and brain. MATERIALS AND METHODS Materials :-Fresh vegetable: Celery (Apium graveolens), broccoli (Brassico oleracea) and green pepper (Capsicum annum) were obtained from the local markets and were dried at 60 °C, and crushed to powder. Oil used several time for frying for not less about 50 hours. Experimental animals; this work was carried out 45 adult male of white albino mices of Sprague dawely strain. The mean weight of male rats ranged from 16 to 19 g. Provided from National Research Center, Cairo, Egypt, were housed as groups in wire cages under the normal laboratory conditions and fed on basal diet according to NRC 1995. Food and water was provided adlibtum. The standard diet was composed as following :-200g casein, 497g Corn starch, 100g Sucrose, 20g Vitamin mixture, 100g Mineral mixture, 50g Corn oil, 3g DL-methionine, 30g Cellulose. Methods :-Chemical Analysis :-Moisture, ash, crude protein, crude fat, and crude fiber were determined according to the methods of the (A.O.A.C.1990). While, total carbohydrates were estimated by subtracting the difference from initial weight of the samples as follows : % Carbohydrates = 100-(% moistur...