Antioxidant Activity and Alpha-Glucosidase Inhibitory Activity of Stings of Gleditsia sinensis Extracts

Abstract

This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on α-glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The α-glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.

Effect of Gleditsia sinensis Lam. Extract on Physico-Chemical Properties of Emulsion-Type Pork Sausages

“…extract contains a lot of phenolic compounds and flavonoids, including ethyl gallate, caffeic acid, dihydrokaempferol, eriodictyol, quercetin, 3,3',5',5,7-pentahydroflavanone and (−)-epicatechin. Lee et al (2011) also determined the total phenol content of Gleditsia sinensis Lam. extract to be 1.12 g / 100 g for methanol extraction and 0.60 g/100 g after ethanol extraction.…”

“…extract showed higher values than the addition of 0.05% sodium ascorbate. According to Lee et al (2011) , the DPPH radical scavenging activities of 0.1% Gleditsia sinensis Lam. extract were 68.8% (extracted with methanol) and 70.4% (extracted with ethanol), respectively.…”

“…Its thorns, called “Jo Gak Ja” in Korea, can be gathered regardless of the season, and have been used in traditional herbal medicine for the treatment of various diseases ( Park et al , 2011 ). Previous studies reported the various biological effects of Gleditsia sinensis Lam., including anti-diabetic, anti-hyperglycemic, antioxidant activity, antiinflammatory, anticancer, anticoagulant activities ( Ha et al , 2008 ; Ko et al , 2007 ; Lee et al , 2011 ; Yoo et al , 2010 ). However, no studies have yet investigated the effects of Gleditsia sinensis Lam.…”