Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract

Abstract

We evaluated the antioxidant activity and anti-wrinkle effects of Aceriphyllum rossii leaf ethanol extract (ARLEE) in vitro using human dermal fibroblasts. The total polyphenol and flavonoid contents of ARLEE were 578.6 and 206.3 mg/g, respectively. At a concentration of 250 μg/mL, the electron-donating ability of ARLEE was 87.1%. In comparison with the vehicle, ARLEE treatment at 100 μg/mL significantly increased type I procollagen synthesis (p < 0.01) by 50.7%. In vitro ARLEE treatment (10 mg/mL) inhibited collagenase and elastase activity by 97.1% and 99.2%, respectively. Compared with the control, ascorbic acid treatment at 100 μg/mL significantly decreased matrix metalloproteinase (MMP)-1 protein expression (p < 0.01) by 37.0%. ARLEE treatment at 50 μg/mL significantly decreased MMP-1 protein expression (p < 0.01) by 46.1%. Ascorbic acid and ARLEE treatments at 100 μg/mL significantly decreased MMP-1 mRNA expression (p < 0.01) by 26.1% and 36.1%, respectively. From these results, we conclude that ARLEE has excellent antioxidant activity and even better anti-wrinkle effects than ascorbic acid in human dermal fibroblasts. These results suggest that ARLEE could be used in functional cosmetics for the prevention or alleviation of skin wrinkles induced by ultraviolet rays.

Anti-wrinkle effect of fermented black ginseng on human fibroblasts

“…Cell viability assay. The HS68 human fibroblasts were seeded onto 96-well plates at a density of 5x10 4 cells/well and incubated for 48 h, as previously described (10). After 48 h of incubation, various concentrations of FBG (10,25,50, 100, and 200 µg/ml in fresh medium) or distilled water (DW; control) were used to treat the cells.…”

“…The HS68 human fibroblasts were seeded onto 96-well plates at a density of 5x10 4 cells/well and incubated for 48 h, as previously described (10). After 48 h of incubation, various concentrations of FBG (10,25,50, 100, and 200 µg/ml in fresh medium) or distilled water (DW; control) were used to treat the cells. The cells were further cultured for 48 h. Subsequently, 200 µl of MTT (0.5 mg/ml MTT in fresh medium) was added to each well followed by incubation at 37˚C for 3 h. The MTT medium was removed by aspiration and 200 µl of dimethyl sulfoxide was added to each well.…”

“…The aging of the skin is induced by complex processes, including intrinsic (e.g., genetic mutation, cellular metabolism and hormonal changes) and extrinsic factors [e.g., chemicals, toxins, pollutants and ultraviolet (UV) radiation] (5,(9)(10)(11). Aging skin is mainly associated with the general atrophy of ECM components with a decrease in the number of fibroblasts, reduced levels of collagen and elastin, and the disorganization of collagen fibrils and elastin fibers (12)(13)(14).…”

“…Alterations in the levels of collagen and elastin primarily cause clinical symptoms of aging skin, such as wrinkles, sagging and laxity (15). The degradation of collagen and elastin in aged or photodamaged (UV-irradiation) skin is associated with matrix metalloproteinases (MMPs) released from epidermal keratinocytes and dermal fibroblasts (10,(14)(15)(16)(17).…”

Anti-Photoaging Effects ofAngelica acutilobaRoot Ethanol Extract in Human Dermal Fibroblasts

“…These are collectively referred to as retinoid dermatitis (9). Consequently, plant-derived extracts that can induce anti-wrinkle effects have recently begun to receive much attention (10,11). …”