Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Abstract

Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folinciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H induced oxidative stress in HepG2 cell line.2 O2 in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2 Results:The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H +ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels. 2O 2 Conclusion:Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported. treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.

Liquorice (glycyrrhiza glabra) hydroethanolic extracts effectively alleviate thioacetamide -induced hepatotoxicity and hematotoxicity in experimental rats through their antioxidant nature

“…The study results are consistent with a previous study in which the level of hepatic MDA greatly increased in TAA group compared to control group and SL treated group as stander. (Bashandy et al, 2018) Administration of LQ hydroethanolic extract at both doses significantly reduced elevated level of MDA, These results are in accordance with Shinde et al, (2016) who studied the antioxidant activity and antiproliferative action of methanolic extract of liquorice (Glycyrrhiza Glabra) in Hepg2 cell line and observed that liquorice treated HepG2 cells showed lower malondialdehyde (MDA) and higher glutathione and catalase levels as compared to only H2O2 treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed. TAA group showed a significant reduction in the CAT, GSH, SOD levels compared to control group and SL treated group as stander, our results are in accordance with those of Fazal et al, (2015) who studied oxidative stress and anti-oxidant status in thioacetamide-induced hepatotoxicity in albino wister rats and reported that TAA treatment results in increase lipid peroxidation and decrease GSH, SOD and CAT than the normal, and this produces oxidative stress in the liver.…”

Structure Based docking studies towards exploring potential anti-androgen activity of selected phytochemicals against Prostate Cancer

“…Glabranin, a flavanone (sub class of flavonoids) derived from Glycyrrhiza glabra L. leaves is well known for its antioxidant, anti-genotoxic and anti- inflammatory activities and various patents have been claimed for its role in stimulating hair growth and cosmetics 36 – 40 . Glabranin fulfils all the drug likeness and ADMET (absorption, distribution, metabolism, excretion and toxicity) properties and emerges as novel potent anti-chemotherapeutic agent in our analysis.…”

Phytochemical Analysis, Liquid Chromatography, and Mass Spectroscopy and in Vitro Anticancer Activity of Annona Squamosa Seeds Linn.

“…Solvent A (water+0.1% formic acid) and Solvent B (acetonitrile) with column Chromolith -50 × 4.6 mm were used for analysis. The fragmentor voltage 135 V, run time 30 min, and flow rate 0.5 ml/ min were maintained [13,14]. In a total run of 30 min in the LCMS, approximately 16 compounds were detected.…”