Antioxidant activity and composition of propolis obtained by different methods of extraction

Cottica, Solange Maria; Sawaya, Alexandra Christine Helena Frankland; Eberlin, Marcos N.; Franco, Selma Lucy; Zeoula, Lúcia Maria; Visentainer, Jesuí V.

doi:10.1590/s0103-50532011000500016

Cited by 97 publications

(92 citation statements)

References 15 publications

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“…Castro et al (2007) found total phenol compound values in propolis produced by A. mellifera bees similar to the values found in propolis produced by A. mellifera bees from the city of Santa Maria of Tocantins. However, Rodríguez et al (2012) and Cottica et al (2011) found higher values for propolis produced by this species. No studies were found showing total phenol concentrations as high as that from the propolis produced by M. fasciculata -city of Santa Maria.…”

Section: Discussionmentioning

confidence: 60%

Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

et al. 2016

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The composition of propolis depends on time, vegetation and the location of the collection area. The objective of this study was to determine the physicochemical characteristics, the concentration of phenol compounds and the antioxidant capacity of propolis of native stingless bees (Meliponinae) and Apis from the State of Tocantins. Extraction with 80% ethanol (v/v) was performed in order to obtain the extracts. Parameters examined were: propolis mass loss by desiccation at 105 ºC, ashes, wax concentration and pH. Furthermore, the propolis antioxidant activity was measured, as well as the total concentration of phenol compounds. The extracts were also analyzed by high performance liquid chromatography. The total concentration of phenol compounds varied between 121.78 and 631.29 (mg GAE g . High performance liquid chromatography analysis allowed us to infer the existence of phenol compounds. The results indicated that the studied propolis samples constitute good sources of natural antioxidants. The variety of phenol compounds identified in this study, and the diverse biological functions reported in literature for these compounds indicated that this stingless bee propolis (Meliponinae) and Apis has a high pharmacological potential. KEYWORDS: Propolis, antioxidant, phenol compounds.Propriedades físico-químicas e capacidade antioxidante de própolis de abelhas sem ferrão (Meliponinae) e Apis de duas regiões do Tocantins, Brasil RESUMO A composição da própolis depende do tempo, da vegetação e a localização da área de coleta. Este estudo teve como objetivo determinar as características físicas e químicas, o teor de compostos fenólicos e capacidade antioxidante da própolis de abelhas nativas sem ferrão (Meliponinae) e Apis do Estado do Tocantins. Para a obtenção dos extratos empregou-se extração com etanol 80% (v/v). Os parâmetros analisados foram: perda de peso por dessecação a 105 °C, cinzas, Teor de cera e pH. Além dessas foi mensurada a atividade antioxidante e o teor de compostos fenólicos totais. Adicionalmente os extratos de própolis foram também analisados por cromatografia líquida de alta eficiência. O teor de compostos fenólicos totais variou entre 121,78 e 631,29 (mg GAE g -1 ). A atividade antioxidante expressa pelo valor de CE 50 variou entre 29,81 e 845,38 µg mL -1 . As análises por cromatografia líquida de alta eficiência permitiram inferir a existência de compostos fenólicos. Os resultados indicaram que as amostras de própolis estudadas constituem boas fontes de antioxidantes naturais. A variedade de compostos fenólicos identificada neste estudo, e as diversas funções biológicas relatadas na literatura para estes compostos, indicaram que a própolis de abelhas sem ferrão (Meliponinae) e Apis tem um grande potencial farmacológico. PALAVRAS-CHAVE: Própolis, antioxidante, compostos fenólicos. Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apis from two regions of Tocantins, Brazil ACTA

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Section: Discussionmentioning

confidence: 60%

Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

et al. 2016

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“…Although propolis has shown beneficial effects in human and animal health by acting as an antioxidant (COTTICA et al, 2011), antimicrobial (FALCÃO et al, 2014 and antiviral (SCHNITZLER et al, 2009), for example, there is no consensus regarding its beneficial effects on poultry performance. Improvements in poultry performance by propolis and its products have been demonstrated in several studies (DENLI et al, 2005;GALAL et al, 2008;SHALMANY;SHIVAZAD, 2006;TEKELI et al, 2011), whereas Eyng et al (2014 showed a decrease in body weight gain and feed intake when broilers from 1 to 7-d of age fed ethanolic propolis extract.…”

Section: Discussionmentioning

confidence: 99%

“…Propolis has important biological properties such as antioxidant (COTTICA et al, 2011), immunomodulatory (FREITAS et al, 2011;YUAN et al, 2012), anti-inflammatory (HORI et al, 2013), antiviral (SCHNITZLER et al, 2009) and antibacterial (FALCÃO et al, 2014) which are mainly attributed to its flavonoids and polyphenols. These compounds are extracted from propolis for use in human medicine where solvents are employed, typically grain alcohol, in order to obtain its ethanol extract.…”

Section: Introductionmentioning

confidence: 99%

Propolis residue inclusion in the diet affects digestive enzyme activity in broiler chickens

Duarte

Eyng

Murakami

et al. 2017

SCA

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This study investigated the effects of the inclusion of solid propolis residue (PR) from alcoholic propolis extraction in the diets of broiler chickens from 1 to 21-d of age on their performance, intestinal morphology, and digestive enzyme activity. 405 male chicks were distributed in a completely randomized design with five treatments (inclusion of 0, 1, 2, 3 and 4% PR in the diets), and three replications with 27 birds each. The birds were fed experimental diets from 1 to 21-d of age and basal diet from 22 to 42-d of age. Feed intake (from 1 to 21-d and 1 to 42-d of age) decreased linearly with increasing levels of PR inclusion (P < 0.05). Dietary inclusion of PR did not affect intestinal morphology at 7 and 21-d of age (P > 0.05). Pancreatic amylase activity presented a quadratic response at 7 and 21-d of age, with its lowest activity estimated at 2.45 and 1.91% PR inclusion, respectively. At 7-d of age, trypsin activity decreased linearly, whereas chymotrypsin activity varied quadratically, with its activity estimated at 2.06% PR inclusion. Intestinal maltase activity varied quadratically with lowest activity predicted at 1.57% PR inclusion at 21-d of age. The dietary inclusion of solid propolis residue of propolis decreases feed intake in broilers and modulates their intestinal and pancreatic enzyme activity. Key words: Disaccharidases. Intestinal morphometry. Pancreatic enzyme. Propolis byproduct. ResumoEste estudo investigou os efeitos da inclusão do resíduo sólido da extração alcoólica da própolis (PR) em dietas de frangos de corte de 1 a 21 dias de idade no desempenho, morfologia intestinal e atividade de enzimas digestivas. 405 frangos machos foram distribuídos em um delineamento inteiramente casualizado com cinco tratamentos (inclusão de 0, 1, 2, 3 e 4% de PR na ração), e três repetições com 27 aves cada. As aves receberam as dietas experimentais de 1 a 21 dias de idade e dieta basal de 22 a 42 dias de idade. O consumo de ração (1 a 21 dias e 1 a 42 dias) diminuiu linearmente com o aumento de inclusão de PR (P < 0,05). A inclusão de PR não afetou a morfometria intestinal aos 7 e 21 dias de idade (P > 0.05). A atividade da amilase pancreática apresentou resposta quadrática aos 7 e 21 dias de idade (P < 0,05), com menores atividades estimadas ao nível de 2,45 e 1,91% de inclusão de PR, respectivamente. Aos 7 dias de idade, a atividade da tripsina diminuiu linearmente, enquanto

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“…DPPH EC50 value between 47 and 160 μg mL -1 for Brazilian hydroalcoholic propolis extracts have been reported [23]. The values between 65 and 79% of DPPH inhibition for Canadian propolis ethanol extracts at 210 μg mL -1 was also reported [24].…”

Section: Figurementioning

confidence: 97%

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Rosli¹,

Roslan²,

Omar³

et al. 2016

AIP Conference Proceedings

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Abstract. Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

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Antioxidant activity and composition of propolis obtained by different methods of extraction

Cited by 97 publications

References 15 publications

Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

Physicochemical properties and antioxidant capacity of propolis of stingless bees (Meliponinae) and Apisfrom two regions of Tocantins, Brazil

Propolis residue inclusion in the diet affects digestive enzyme activity in broiler chickens

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

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