Antioxidant Activity and Cytotoxicity of the Traditional Indonesian Medicine Tahongai (Kleinhovia hospita L.) Extract

Abstract

We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50 microg/mL, 87.5 microg/mL, and 125 microg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.

Antioxidative compounds from leaves of Tahongai (Klienhovia hospita)

“…9 250 mm), yielded compound 1 (5 mg, yield 0.00099%, Fig. 1) named kaempferol 3-O-b-D-glucoside or astragalin which was identical with reported data [6].…”

“…In our previous study, we reported that ethyl acetate fraction showed antioxidant in DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay but not antiproliferation effect on HepG2 cells [6]. In this study, we isolated two compounds from ethyl acetate fraction and evaluated their antioxidant activities using DPPH radical scavenging assay and their antiproliferation effects on HepG2 cells.…”

“…To determine cells viability, MTT assay was used as described by Arung et al [6] with minor modification.…”

Prospecting for Bioactive Constituents from Traditional Medicinal Plants through Ethnobotanical Approaches

“…5) was isolated from the roots of C. robustum, which is used as a traditional Chinese medicine to treat external injuries and irregular menses, and showed good scavenging effects against DPPH radicals with an IC 50 value of 39 µM. 58) Furthermore, numerous extracts from TMPs showed marked antioxidant activity, such as Kleinhovia hospita, 59) Orthosiphon stamineus, 60) Anogeissus acuminata, 61) etc.…”

Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells

“…Indonesia with its wide biodiversities has plenty of resourceful natural materials those should be explored. Previously our team reported several potential extracts derived from plants including Ipomea batatas (1)(2)(3), Artocarpus altilis (4), Piper crocatum (5), Kleinhovia hospita (6), Artocarpus heterophyllus (7), Catharanthus roseus (8), Piper betle (9), Catharanthus roseus (9), Dendrophtoe petandra (9), Curcuma mangga (9), Curcuma longa (10), Ananas comosus (11), Brucea javanica (12,13), Nephelium lappaceum (14), Cucumis melo (15,16), Caesalpinia sappan (17) and Artocarpus elasticus (18). These extracts shows various activities, including osteoclastogenesis inhibition (1-3), apoptosis induction or proliferation Results inhibition on breast cancer cells (4,5,(7)(8)(9), antioxidant (6,14,16,18), proliferation and differentiation induction on stem cell (10,15), and apoptosis induction on human oral squamous cell carcinoma (12,13,16,17).…”