Antioxidant Activity and Quantitative Composition of Extracts of Piper Species From Guatemala With Potential Use in Natural Product Industry

Cáceres, Armando; Cruz, Sully M.; Gaitán, I.; Guerrero, Katherine Andrea Sánchez; Alvarez, Luis; Marroquín, M.N.

doi:10.17660/actahortic.2012.964.9

Cited by 11 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This report is the first of an antifungal effect of P. neesianum against F. equiseti and F. oxysporum. The dichloromethane extract from leaves of P. neesianum has been reported to have various biological activities as an antioxidant (IC 50 = DPPH 0.071 mg/mL) [ 54 ], anti-tyrosinase (IC 50 = 6.6 µg/mL [ 55 ] and anti-urease (IC 50 = 12.9 µg/mL) [ 56 ]. Essential oil from P. neesianum leaves collected in the northern region of Guatemala contained bicyclogermacrene (28%), germacrene D (11.7%) and β-caryopyllene (7.5%) as major compounds among 19 detected in a gas chromatography with flame ionization detection- mass spectrometry analysis [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

et al. 2020

View full text Add to dashboard Cite

Fusarium equiseti strain FCHE and Fusarium oxysporum strain FCHJ were isolated from the roots of wilting habanero pepper (Capsicum chinense Jacq.) seedlings with root rot. Toward developing a biorational control of these serious phytopathogenic strains, ethanolic (EE) and aqueous (AE) extracts of different vegetative parts of 40 tropical native plants of the Yucatán Peninsula were screened for antifungal activity. Extracts of six out of 40 assayed plants were effective, and the most inhibitory extracts were studied further. EEs from Mosannona depressa (bark from stems and roots), Parathesis cubana (roots), and Piper neesianum (leaves) inhibited mycelial growth of both strains. Each active EE was then partitioned between hexane and acetonitrile. The acetonitrile fraction from M. depressa stem bark (MDT-b) had the lowest minimum inhibitory concentration of 1000 µg/mL against both pathogens and moderate inhibitory concentration (IC50) of 462 against F. equiseti and 472 µg/mL against F. oxysporum. After 96 h treatment with EE from M. depressa stem bark, both strains had distorted hyphae and conidia and collapsed conidia in scanning electron micrographs. Liquid chromatography–ultraviolet–high resolution mass spectrometry analysis revealed that the major component of the fraction was α-asarone. Its antifungal effect was verified using a commercial standard, which had an IC50 of 236 µg/mL against F. equiseti and >500 µg/mL against F. oxysporum. Furthermore, the P. cubana hexane fraction and P. neesianum acetonitrile fraction had antifungal activity against both Fusarium pathogens. These compounds provide new options for biorational products to control phytopathogenic fungi.

show abstract

Section: Discussionmentioning

confidence: 99%

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Many of these metabolites have biological and pharmacological activities that are used by communities, through the use of plants for the treatment of different diseases [1]. In this way, many investigations have been carried out aimed at determining the composition and biological activity of the metabolites of different medicinal plants used in Guatemala [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Seed Propagation and Constituents of the Essential Oil ofStevia serrataCav. from Guatemala

Pérez-Sabino¹,

Mérida-Reyes²,

Martínez-Arévalo³

et al. 2020

Essential Oils - Oils of Nature

View full text Add to dashboard Cite

Stevia serrata Cav. (Eupatorieae, Asteraceae) grows in Central America and Mexico usually over 1500 m. In this study, essential oils of aerial parts from three populations of western Guatemala were obtained yielding 0.17-0.27% of oil by hydrodistillation. Chamazulene (42-62%) was the most abundant compound in the oil analyzed GC/MS, also presenting germacrene D (4.4-15.3%), caryophyllene oxide (3.2-11.8%), (E)-nerolidol (3.9-7.1%), spathulenol (2.3-7.9%), and (E)-caryophyllene (2.5-6.6%). Besides, a propagation trial was carried out on seeds of plants collected in Santa Lucía Utatlán, as the first step for the domestication of the plant, obtaining approximately 75% survival in the transplanting of the germinated seedlings. After the flowering of the individuals, a greenish essential oil was obtained from the roots yielding 0.2% of oil. This oil did not present chamazulene, but α-longipinene (23.5%), germacrene D (22.2%), santolina triene (12.6%), and (E)-caryophyllene (8.1%) as major components. As conclusion, it was confirmed that the aerial parts of the essential oil of S. serrata from western Guatemala presents a high content of chamazulene and that there is feasibility for the domestication of the plant through the germination of seeds.

show abstract

“…4 Previous studies have demonstrated that its leaves have antioxidant activity. 5 The essential oil contains as major components 1,3,5-trimethoxy-2-propenylbenzene (33.7%), methyl eugenol (24.7%) and -caryophyllene (16.2%), 6 has shown cytotoxic activity against brine shrimp (Artemia salina) at a concentration of 0.5 mg/mL, 7 and contain distinctive features to characterize micro and macroscopically. 8 This research seeks to find an organic fraction of P. patulum with cytotoxic activity, for the future development of a molecule as a potential accessible drug with fewer side effects.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antioxidant, genotoxic and cytotoxic activity of organic fractions from leaves and roots of Piper patulum Bertol. from Guatemala

Zelada¹,

Cáceres²,

Carvalho³

2016

Int J Phytocosmetics Natu Ingredients

View full text Add to dashboard Cite

Piper patulum Bertol., is a native Mesoamerican species. Previous studies have demonstrated that its leaves have antioxidant activity and the essential oil has shown cytotoxic activity against brine shrimp (Artemia salina) at a concentration of 0.5 mg/mL. The aim of this research was to demonstrate the antioxidant, cytotoxic and genotoxic activity of fractions of the vegetal material. Fractions were obtained by separate sequential extraction of leaves and roots. Phytochemicals were evaluated to determine the qualitative composition, suggesting a possible pattern to continue the study of cytotoxic activity. Qualitative antioxidant activity was evaluated by TLC based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) test, and quantitative DPPH and total phenolics by spectrophotometry. Cytotoxic activity against A. salina was evaluated macrometrically, and towards cancer cell lines [U251 (glioma), MCF-7 (mammary) and NCI-H460 (lung)] by micrometric sulforhodamine B assay, estimating the Total Growth Inhibition (TGI). Genotoxicity was tested by the Allium cepa assay. The dichloromethane fraction from the roots showed the best antioxidant activity (IC 50 of 0.61 ± 0.04 mg/mL) and the greatest concentration of total phenolics (848.42 ± 9.89 µg of gallic acid equivalents/mL). Cytotoxic activity was determined against A. salina and neoplastic cell lines. For A. salina, the highest activity was observed in the methanol (DL 50 0.26 ± 0.02 mg/mL) and dichloromethane (DL 50 0.29 ± 0.02 mg/mL) organic fractions from the roots. In cytotoxic assays against cancer cell lines, the best TGI were obtained in the dichloromethane (U251: 68.9 µg/ mL, MCF-7: 23.5 µg/mL, NCI-H460: 74.4 µg/mL) and ethyl acetate leaf fractions (U251: 53.8 µg/mL, MCF-7: 42.0 µg/mL, NCI-H460: 71.6 µg/mL), and the dichloromethane root fraction (U251: 94.2 µg/mL, MCF-7: 31.0µg/mL, NCI-H460: 88.6 µg/mL). By means of the A. cepa assay, no genotoxic activity was demonstrated. The dichloromethane fraction of the leaf from P. patulum shows interesting cytotoxic activity, which deserves further investigation of a responsible molecule by bioguided fractionation.

show abstract

Antioxidant Activity and Quantitative Composition of Extracts of Piper Species From Guatemala With Potential Use in Natural Product Industry

Cited by 11 publications

References 0 publications

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

Seed Propagation and Constituents of the Essential Oil ofStevia serrataCav. from Guatemala

Evaluation of the antioxidant, genotoxic and cytotoxic activity of organic fractions from leaves and roots of Piper patulum Bertol. from Guatemala

Contact Info

Product

Resources

About