Antioxidant Activity and Total Polyphenols Content of Camellia Oil Extracted by Optimized Supercritical Carbon Dioxide

Abstract

In this study, Camellia oil is co‐extracted from Camellia oleifera seeds and green tea scraps by supercritical carbon dioxide (SC‐CO2), which is optimized on the extraction yield, ABTS‐scavenging activity, and total polyphenols content (TPC) of oil by single‐factor experiments combined with response surface methodology (RSM). The extraction temperature, pressure, dynamic time, carbon dioxide (CO2) flow rate, and seed mass ratio were investigated with single‐factor experiments. The results indicated the optimum CO2 flow rate and dynamic extraction time were 15 L hour−1 and 60 min (i.e., 2.382 kg CO2/100 g sample). Furthermore, the complicated effects of extraction temperature (40–50 °C), pressure (20–30 MPa), and seed mass ratio (0.25–0.75) were optimized by RSM based on the Box–Behnken design (BBD). The models with high R‐squared values were obtained and used to predict the optimum operating conditions of the process. Under the optimum operating conditions (i.e., temperature of 46 °C, pressure of 30 MPa, and seed mass ratio of 0.35), the extraction yield, ABTS‐scavenging activity, and TPC of oil were 14.43 ± 0.17 g/100 g sample, 73.70 ± 0.34%, and 2.18 ± 0.05 mg GAE/g oil, which were in good agreement with the predicted values. In addition, the experiments indicated that the Camellia oil obtained was rich in polyphenols, resulting in better oxidation stability and antioxidant activity than the original oil.

Comparison of Antioxidant and Antibacterial Activities of Camellia Oil From Hainan With Camellia Oil From Guangxi, Olive Oil, and Peanut Oil

“…TPC of SC and TFC of SY were the highest in the four vegetable oils. This result was consistent with the reference ( 41 ), and TPC of SC and SY was higher than that reported by Wang et al (2.18 mg GAE/g oil) ( 13 ). It was possible that the tea seeds of our sample were physically pressed by shelling, while Wang's sample was extracted with supercritical carbon dioxide.…”

“…Camellia oil is rich in unsaturated fatty acids ( 2 , 10 ), such as oleic acid, linoleic acid; Sesamin ( 11 ), saturated acids ( 12 ), polyphenols ( 13 ) also are found in camellia oil. These compounds shows excellent antioxidant activities.…”

“…These compounds shows excellent antioxidant activities. So, Lee and Yen found methanol extract of camellia oil exhibited DPPH· scavenging activity ( 11 ); Wang et al ( 10 ) and Wang et al ( 13 ) reported Supercritical Carbon Dioxide extract of camellia oil also showed a stronger DPPH· and ABTS· scavenging activity; the antioxidant activities from the literatures were most limited in DPPH· and ABTS· scavenging activity in vitro , were not involved to treat with Saccharomyces cerevisiae in vivo .…”

Comprehensive Evaluation of Quality of <i>Camellia semiserrata</i> Seed Oils from Various Harvest Dates

“…The polyphenols content in CSOs was determined by Folin-Ciocalteau method 18) . Firstly, polyphenols were extracted from CSO (3.0 g) with 5 mL 80％ methanol three times.…”

Safety Assessment of Aqueous and Supercritical CO2 Extracts of the Chaga Mushroom Inonotus obliguus

“…Несмотря на многочисленные существующие способы экстракции гриба Inonotus obliquus, основанные на использовании разных растворителей, сведения об использовании сверхкритического диоксида углерода в технологии получения экстрактов березового гриба отсутствуют. Экстракционная способность диоксида углерода в до-и сверхкритическом состоянии представлена в работах отечественных и зарубежных авторов [5][6][7][8][9][10] Для проведения анализа на токсичность по методике, указанной в пособии авторов [12], готовили ряд пробирок (в 3-х параллелях), содержащих 2 мл углеводно-солевой дрожжевой среды (УСД), в которые внесли по 1 мл сверхкритического СО 2 -экстракта или 0,5 % к массе среды УСД сухого экстракта. Контролем в этом эксперименте служат 3 параллельных пробирки с 2 мл УСД, в которые добавляется 1 мл физиологического раствора.…”