Antioxidant Activity and Toxic Effects of Phosphorus-Containing Derivatives of 2,6-Di-tert-Butylphenol in silico and in vitro, in vivo

Половинкина, М. А.; Осипова, В. П.; Kolyada, M. N.; Osipova, A. D.; Берберова, Н. Т.; Pimenov, Yuri T.; Милаева, Е. Р.

doi:10.5755/j01.erem.75.1.21050

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…While caprolactam had the highest level among antagonists and rhodamine 6G exhibited the strongest potency, 8-hydroxyquinoline stood out owing to its top rankings in both concentration (second) and REP (fifth) (Table S7). Triphenyl phosphate and 2,6-di tert -butylphenol (an antioxidant) were, respectively, important contributors in Hefei and Xinyu, each covering 6.1 and 14% of total effects. Industrial reagent 2,6-di tert -butylphenol ranked the fourth major contributor in the Xinyu sample, covering 14% of BEQ, its highest contribution across all 30 samples.…”

Section: Resultsmentioning

confidence: 99%

Occurrence and Prioritization of Human Androgen Receptor Disruptors in Sewage Sludges Across China

Xiang,

Liu,

Guo

et al. 2024

Environ. Sci. Technol.

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The global practice of reusing sewage sludge in agriculture and its landfill disposal reintroduces environmental contaminants, posing risks to human and ecological health. This study screened sewage sludge from 30 Chinese cities for androgen receptor (AR) disruptors, utilizing a disruptor list from the Toxicology in the 21st Century program (Tox21), and identified 25 agonists and 33 antagonists across diverse use categories. Predominantly, natural products 5α-dihydrotestosterone and thymidine emerged as agonists, whereas the industrial intermediate caprolactam was the principal antagonist. In-house bioassays for identified disruptors displayed good alignment with Tox21 potency data, validating employing Tox21 toxicity data for theoretical toxicity estimations. Potency calculations revealed 5α-dihydrotestosterone and two pharmaceuticals (17β-trenbolone and testosterone isocaproate) as the most potent AR agonists and three dyes (rhodamine 6G, Victoria blue BO, and gentian violet) as antagonists. Theoretical effect contribution evaluations prioritized 5α-dihydrotestosterone and testosterone isocaproate as high-risk AR agonists and caprolactam, rhodamine 6G, and 8-hydroxyquinoline (as a biocide and a preservative) as key antagonists. Notably, 16 agonists and 20 antagonists were newly reported in the sludge, many exhibiting significant detection frequencies, concentrations, and/or toxicities, demanding future scrutiny. Our study presents an efficient strategy for estimating environmental sample toxicity and identifying key toxicants, thereby supporting the development of appropriate sludge management strategies.

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Section: Resultsmentioning

confidence: 99%

Occurrence and Prioritization of Human Androgen Receptor Disruptors in Sewage Sludges Across China

Xiang,

Liu,

Guo

et al. 2024

Environ. Sci. Technol.

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“…Russian sturgeon's sperm LPO intensity has been assessed by carbonyl oxidation byproducts accumulation, which reacted with thiobarbituric acid (TBARS) using the published method [26]. Determination method of TBARS level in sturgeon sperm before cryopreservation with cryomedium and after cryopreservation with cryomedium also was published earlier [27].…”

Section: Determination Of Russian Sturgeon's Sperm Lipid Peroxidation...mentioning

confidence: 99%

Application of a synthetic phenol-type antioxidant for cryopreservation of Russian sturgeon (Acipenser gueldenstaedtii) sperm

Osipova,

Kolyada,

Polovinkina

et al. 2023

E3S Web Conf.

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Antioxidative and cryoprotective effects of a novel synthetic antioxidant–1,1'-(piperazine-1,4-diyl)bis(2-(2-hydroxyphenylthio)ethanone (AO) is presented in comparison with commonly known antioxidant butylated hydroxytoluene (BHT). Superoxide anion radical scavenging activity of new AO and the increase in superoxide dismutation activity of the Russian sturgeon’s (Acipenser gueldenstaedtii) sperm were established in the presence of this compound. It was shown that the new phenol agent reduces level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in Russian sturgeon’s native and frozen/thawed sperm, which indicates the manifestation of antioxidant properties by this compound including under the action of damaging factors during cryopreservation. A beneficial effect of this phenol derivative on the activity indicators (the percentage of motile sperm cells and total period of sperm movement) of the Russian sturgeon’s frozen/thawed sperm was shown, which indicates the cryoprotective effect of a novel synthetic antioxidant. The efficiency of the AO exceeds the effect of BHT under cryopreservation conditions of Russian sturgeon sperm in the presence of the modified Stein’s medium, but further studies are needed on the effect of a new phenol agent on the fertilizing capacity of Russian sturgeon’s sperm.

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“…The intensity of sperm lipoperoxidation was assessed by the accumulation of carbonyl oxidation by-products, which react with thiobarbituric acid (TBARS), using the traditional method as described previously (Osipova et al, 2017;Polovinkina et al, 2019). The content of TBARS was expressed as nanomoles per 10 9 cells.…”

Section: Determination Of the Accumulation Level Of Tbars In Russian mentioning

confidence: 99%

The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

Kolyada

Осипова

Берберова

et al. 2020

EREM

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The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products, which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH3SnCl3, (CH3)2SnCl2, (CH3)3SnCl, (n-C4H9)2SnCl2, (n-C4H9)3SnCl, (C6H5)2SnCl2, (C6H5)3SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl- (DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein’s cryomedium decreased in the presence of the studied OTs. The results suggest that accumulation of OTs by gonad of fish is another stress factor affecting the cell productivity in the cryopreservation process.

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Antioxidant Activity and Toxic Effects of Phosphorus-Containing Derivatives of 2,6-Di-tert-Butylphenol in silico and in vitro, in vivo

Cited by 5 publications

References 28 publications

Occurrence and Prioritization of Human Androgen Receptor Disruptors in Sewage Sludges Across China

Occurrence and Prioritization of Human Androgen Receptor Disruptors in Sewage Sludges Across China

Application of a synthetic phenol-type antioxidant for cryopreservation of Russian sturgeon (Acipenser gueldenstaedtii) sperm

The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

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