The Effect of Tin Compounds on the Lipid Peroxidation Level of Russian Sturgeon Fresh and Cryopreserved Sperm

Abstract

<jats:p>The effect of organotin compounds (OTs) on the accumulation of the lipid peroxidation (LPO) carbonyl by-products, which react with thiobarbituric acid (TBARS) in fresh and cryopreserved sperm of Russian sturgeon, was studied. It was found that incubation (1 hour) of Russian sturgeon sperm with OTs (CH3SnCl3, (CH3)2SnCl2, (CH3)3SnCl, (n-C4H9)2SnCl2, (n-C4H9)3SnCl, (C6H5)2SnCl2, (C6H5)3SnCl) in concentration 0.1 mM led to the promotion of the accumulation of TBARS in native semen. Dimethyl- (DMT) and diphenyltin dichlorides (DPT) exhibited the greatest promoting activity, and the LPO level of both native and cryopreserved sperm of Russian sturgeon, including those in modified Stein's cryomedium, increased in the presence of these compounds. It was found that Russian sturgeon's cryopreserved sperm had lower sensitivity to the promotion of sperm LPO by DMT and DPT compared with the native sperm. The protective effect of Stein’s cryomedium decreased in the presence of the studied OTs. The results suggest that accumulation of OTs by gonad of fish is another stress factor affecting the cell productivity in the cryopreservation process.</jats:p>

Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

“…Currently, cryopreservation has a detrimental effect on the structural and functional parameters of Russian sturgeon spermatozoa [ 5 , 34 , 35 , 51 ]. Many factors influence the success of cryopreservation, including the initial quality of semen, the composition of the cryomedium, the cryoprotectant, the dilution factor, the concentration of spermatozoa used for cryopreservation, speed of freezing and thawing, as well as physiological aspects of sperm, such as species specificity [ 52 , 53 , 54 ].…”

“…The main causes and direct threats to sturgeon survival in the Danube River are overfishing, natural habitat destruction, pollution, and disruption of sturgeon migration routes. In addition to the legislative and financial measures taken in an attempt to fight off this ongoing natural disaster, sturgeon aquaculture has gained importance over the past years [ 4 , 5 ]. In nature, seasonal factors, such as water temperature and photoperiod play the main role in the annual growth response and maturation cycle of sturgeons.…”

“…The cryopreservation of semen allows the sperm to be stored indefinitely, and this method has been recognized as the most appropriate way for gene banking aimed to preserve specific genetic diversity [ 32 ]. Relatively few studies have focused on the cryopreservation of Russian sturgeon spermatozoa [ 5 , 33 , 34 , 35 ] and there are still many unknown issues that need to be elucidated. Cryopreservation remains one of the most reliable methods for semen storage, and there are a few studies that report successful preservation of sturgeon semen, the majority of them using egg yolk in extenders, which carries the risk of contamination in commercial hatcheries [ 36 ].…”

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

“…One of the other current issues studied in sperm cryopreservation in sturgeon is the effect of organotin components (OTs) on fresh and frozen sperm. It has been reported that the accumulation of OTs in the gonad in Russian sturgeon is a stress factor affecting the cells in the cryopreservation process and also this buildup may cause in vitro oxidative stress in sturgeon sperm, reduce gamete quality, and affect fertilization success [57].…”