Antioxidant activity of BHA, BHT and TBHQ examined with Miller's test

Karamać, Magdalena; Amarowicz, Ryszard

doi:10.3989/gya.1997.v48.i2.772

Cited by 17 publications

(10 citation statements)

References 11 publications

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“…Hexadecane identified in methanol, chloroform, and ethyl acetate extracts is reported for antibacterial as well as antioxidant activities [39]. Butyl hydroxyl toluene, an excellent antioxidant, contributed 9.45% to the diethyl ether extract [40]. A variant of phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol has contributed about 2.73-9.5% in acetone, chloroform and diethyl ether extract.…”

Section: Gc-hrms Analysis Of Crude Organic Extracts Of C Emersonii Kmentioning

confidence: 99%

Nutritional Profile, Antioxidant, Antimicrobial Potential, and Bioactives Profile of Chlorella Emersonii Kj725233

Sawant

Mane

2018

Asian J Pharm Clin Res

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Objective: The present study revolved around the nutritional evaluation of Chlorella emersonii KJ725233, a novel non-fastidious Chlorella strain that was isolated from the Western regions of Maharashtra, India, for a probable use as a food supplement.Methods: The nutritional composition of C. emersonii KJ725233 was evaluated in terms of total protein, total lipids, and total carbohydrates content along with its mineral composition. Furthermore, the effect of different solvents on the extraction of the bioactives and hence the antioxidant and antimicrobial activity of C. emersonii KJ725233 was also analyzed. The bioactives extracted in the organic solvents were further identified by gas chromatography–high-resolution mass spectrometry (GC-HRMS).Results: As reported in the literature for commercial Chlorella powder, C. emersonii KJ725233 was found to contain an equivalent concentration of protein, three-fold lipid content, i.e. 36.48±0.52% and 43.58±0.72%, respectively; along with 17.17±0.91% carbohydrates and 6.62±0.16% mineral content. Methanolic content was not only found to contain the highest antioxidant, radical scavenging potential as well as total phenolic content but also exhibited a strong antimicrobial potential against the tested Gram-positive, Gram-negative bacterial and fungal strains. Moreover, sterols, hydrocarbons, and fatty acid methyl esters known antimicrobials and antioxidants were identified when the organic extracts of the microalgae dried biomass were subjected to GC-HRMS analysis.Conclusion: With a well-balanced nutritional composition together with the reservoir of antioxidants and antimicrobials, this native novel isolate of Chlorella thus emerges as a potential food supplement whose consumption would not only enhance the dietary value along with providing antiaging benefits.

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Section: Gc-hrms Analysis Of Crude Organic Extracts Of C Emersonii Kmentioning

confidence: 99%

Nutritional Profile, Antioxidant, Antimicrobial Potential, and Bioactives Profile of Chlorella Emersonii Kj725233

Sawant

Mane

2018

Asian J Pharm Clin Res

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“…Antioxidant activity was expressed by inhibitory ratio as follows, according to the formula used by Karamac and Amarowicz (1997):…”

Section: Antioxidant Activity Measurementmentioning

confidence: 99%

Effect of the essential volatile oils isolated from <i>Thymbra capitata</i> (L.) Cav. on olive and sunflower oils

Miguel¹,

Figueiredo²,

Costa³

et al. 2003

Grasas y Aceites

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RESUMEN Efecto de aceites volátiles esenciales aislados de Thymbra capitata (L.) Cav. En aceites de oliva y girasol.Se analizaron, mediante GC y GC/MS, los componentes volá-tiles de aceites aislados de las distintas partes de la Thymbra capitata, recogida en diferentes etapas de desarrollo. Se evaluó la actividad antioxidante de estos aceites de la T. capitata, midiendo el índice de peróxidos, en aceites de oliva y girasol, almacenados a 60 o C. Estos índices de peróxidos se compararon con los obtenidos cuando no se agregó ningún antioxidante (control) y cuando se utilizó BHT o carvacrol, en las mismas condiciones de almacenamiento. El mayor rendimiento en aceite se obtuvo de la parte aérea de T. capitata recogida durante la etapa de floración. El componente mayoritario de los aceites fue el carvacrol. También se encontraron, cantidades relativamente elevadas, de p-cimeno, γ-terpineno y β-cariofileno. El mejor antioxidante para el aceite de oliva resultó ser el BHT. En el aceite del girasol, la actividad antioxidante del BHT no fue tan evidente, mientras que el carvacrol y los aceites esenciales de T. capitata ricos en carvacrol mostraron una mayor actividad antioxidante. PALABRAS-CLAVE:Aceite de girasol -Aceite de oliva -Aceites volátiles esenciales -Actividad antioxidante --CarvacrolThymbra capitata. SUMMARY Effect of the essential volatile oils isolated fromThymbra capitata (L.) Cav. on olive and sunflower oils.The chemical composition of the volatile constituents of the oils isolated from different parts of Thymbra capitata collected at different developmental stages were analysed by GC and GC/MS. The antioxidant ability of the oils isolated from T. capitata was evaluated determining the peroxide values, on olive and sunflower oils, stored at 60 o C. These peroxide values were compared with those obtained when BHT, carvacrol and control (without adding antioxidants) were used and subjected to the same conditions. The best yield oil was obtained from the whole aerial part of T. capitata collected during the flowering phase. The major component of the oils was carvacrol. Relative high amounts of p-cymene, γ-terpinene and β-caryophyllene were also found. BHT revealed to be the best antioxidant when the olive oil was used. On sunflower oil, the antioxidant ability of BHT was not so evident, being the carvacrol-rich essential oils of T. capitata or carvacrol more important antioxidants.

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“…In addition, the antioxidative effects of ascorbic acid and ascorbyl palmitate in the O/W emulsions have been fully studied (Van Ruth et al 1999;Liu and Yang 2008;Mosca et al 2008;Watanabe et al 2010;Yang et al 2011). Previous studies have also revealed that tert-butyl hydroquinone (TBHQ) acted effectively as the free radical scavenging agents in emulsions (Karamac and Amarowicz 1997;Kishk and Al-Sayed 2007;Alamed et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Effects of antioxidants on the stability of β-Carotene in O/W emulsions stabilized by Gum Arabic

et al. 2014

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The potential of oil-in-water emulsions as a β-carotene delivery system was examined in this study. Oil-inwater (O/W) emulsions containing β-carotene were formed by gum arabic with α-tocopherol, tertiary butyl hydroquinone (TBHQ) and ascorbyl palmitate, respectively. The influence of antioxidants on the chemical degradation of β-carotene in gum arabic stabilized emulsions was investigated at 4, 25, 45 and 65°C in the dark, respectively. An accelerated photooxidation test was carried out at 45°C (450 W/m 2 ). Moreover, β-carotene degradation rate constants (k 1 -value), activation energy (E a ) and decimal reduction time (D-value) were estimated to interpret the degradation kinetics. The impact of antioxidants on the thermal stability of β-carotene in diluted emulsions was generally in the following order: α-tocopherol> TBHQ >ascorbyl palmitate. α-Tocopherol was found to be the most effective to the antioxidation of β-carotene at the concentration of 0.10 wt% under light exposure. It was concluded that the stability of β-carotene in oil-in-water emulsions could be improved by the presence of different antioxidants.

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Antioxidant activity of BHA, BHT and TBHQ examined with Miller's test

Cited by 17 publications

References 11 publications

Nutritional Profile, Antioxidant, Antimicrobial Potential, and Bioactives Profile of Chlorella Emersonii Kj725233

Nutritional Profile, Antioxidant, Antimicrobial Potential, and Bioactives Profile of Chlorella Emersonii Kj725233

Effect of the essential volatile oils isolated from <i>Thymbra capitata</i> (L.) Cav. on olive and sunflower oils

Effects of antioxidants on the stability of β-Carotene in O/W emulsions stabilized by Gum Arabic

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