Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro

Qi, Huimin; Zhang, Quanbin; Zhao, Tingting; Chen, Rong; Hong, Zhigang; Niu, Xiaofeng; Li, Zhien

doi:10.1016/j.ijbiomac.2005.10.008

Cited by 502 publications

(256 citation statements)

References 34 publications

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“…than k-carrageenan oligosaccharides. Qi et al (2005) found that sulfated acetylated and benzoylated ulvan exhibited stronger antioxidant activity than natural ulvan. Tsipali et al (2001) observed that phosphorylated and sulfated glucans exhibited antioxidant ability than the glucans and other neutral polysaccharides.…”

Section: Resultsmentioning

confidence: 98%

Antioxidant Activity of the Derivatives of Polysaccharide Extracted from a Chinese Medical Herb (Ramulus mori)

Zhang

Jin

Shi

2008

FSTR

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The antioxidant activity of the polysaccharide from Ramulus mori and its derivatives (sulfated, phosphorylated, acetylated and benzoylated polysaccharides) were determined, including scavenging activity against superoxide, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals, reducing power and chelating ability. Obvious differences in antioxidant activity between the natural polysaccharide and its derivatives were observed, the antioxidant activity of the sulfated, phosphorylated and acetylated derivatives was stronger than that of the natural polysaccharide. In addition, the effect of temperature (20, 40, 60, 80 and 90 °C) and pH (3, 5, 7, 9 and 11) on the antioxidant activity of the natural polysaccharide and its derivatives was also investigated. The antioxidant activity of all samples in the linoleate emulsion improved with increasing from pH 3 to 11, while it decreased with ascending storage temperature from 20 to 90 °C.Keywords: antioxidant activity, temperature, pH, polysaccharide derivatives, Ramulus mori *To whom correspondence should be addressed. Email: slgsilk@zju.edu.cn IntroductionReactive oxygen species (ROS) produced by sunlight, ultraviolet light, ionizing radiation, chemical reactions and metabolic processes have a wide variety of pathological effects, such as causing DNA damage, carcinogenesis and cellular degeneration related to aging (Blander et al., 2003). In order to reduce damage of free radicals, synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), propylgallate (PG), and tert-butylhydroquinone (TBHQ) are used. However, BHA and BHT have been suspected of being responsible for liver damage and carcinogenesis (Grice, 1988). Thus, it is essential to develop and utilize effective and natural antioxidants so that they can protect the human body from free radicals and retard the progress of many chronic diseases (Nandita and Rajini, 2004).Polysaccharides are common structural and storage polymers in living organisms, representing more than 75 % of the dry mass of plants. Polysaccharides are potentially useful, biologically active ingredients for pharmaceutical uses due to a variety of biological activities, such as immunological, anti-radiation, anti-blood coagulation, anti-cancer, anti-HIV and hypoglycemic activities (Yang et al., 2005;Yoon et al., 2003;Lee et al., 2002). The activity of polysaccharides is close related to their structure such as molecular mass, degree of substitution, degree of branching, sugar component and the structures of main chain and branches (Bohn and BeMiller, 1995). Thus, chemical modifications of polysaccharides provided an opportunity to obtain new agents with possible therapeutic uses (Franz and Alban, 1995).Ramulus mori is the branch of Morus alba L., family Moraceae, according to the theories of traditional Chinese medicine, it is slightly bitter in taste, mild in nature, and attributive to the liver meridian. In traditional Chinese medicine, its pharmacological actions are to expel wind, dredge the meridians, and ease...

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Section: Resultsmentioning

confidence: 98%

Antioxidant Activity of the Derivatives of Polysaccharide Extracted from a Chinese Medical Herb (Ramulus mori)

Zhang

Jin

Shi

2008

FSTR

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“…The bioactivity of sulfated polysaccharides is dependent on the sulfur DS (Qi et al, 2005). Moderate sulfating modification improved the radical scavenging activity of CBPs significantly, but excessive modification showed an opposite effect.…”

Section: Discussionmentioning

confidence: 99%

Effect of sulfation on the antioxidant properties and in vitro cell proliferation characteristics of polysaccharides isolated from corn bran

Zhou

et al. 2016

CyTA - Journal of Food

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Polysaccharides were extracted from corn bran (CBPs) and then modified by a sulfating reaction to obtain sulfated corn bran polysaccharides (SCBPs). Synthesized sulfated polysaccharides were located in the equatorial bonds of monosaccharide residues. A more orderly and smooth surface of SCBPs was observed. Single factor experiment results showed 2.0 g of aminosulfonic acid, 60 mL of N-dimethylformamide and 2.5 h of reaction time were the optimum conditions for SCBPs preparation. Phenolic content of SCBPs significantly decreased with increasing concentration of aminosulfonic acid, while it increased with increasing DMF concentration and reaction time. SCBPs had higher DPPH, ABTS and iron chelating capacity under the optimum conditions. SCBPs significantly inhibited the proliferation of A549 and HepG2 cell lines, which was associated with the upregulation at the mRNA expression level of pro-apoptotic genes CASP3, CASP8, CASP9 and reduced expression of p53, gene Bcl-2 and gene iNOS.

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“…Superoxide free radical scavenging activity The superoxide radical scavenging assay was assessed according to the method of Qi et al (2005). Superoxide radicals were generated in a PMS/NADH system for being assayed in the reduction of NBT.…”

Section: Methodsmentioning

confidence: 99%

Optimization of extraction conditions for phytic acid from rice bran using response surface methodology and its antioxidant effects

Zhang

Bai

2011

J Food Sci Technol

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Solid-liquid extraction of phytic acid (PA) from rice bran was optimized by the maximization of the yield using response surface methodology. A Box-Behnken design was used to monitor the effects of three processing parameters of extraction on the PA yield, including ratio of acid solution to raw material (mL/g), hydrochloric acid concentration (mol/L), and extraction time (h). The results showed that the optimal conditions were acid solution/raw material of 8.5:1 (mL/g), HCl concentration 0.62 mol/L and extraction time 5.5 h. Validation tests indicated that the actual yield of PA was (2.15± 0.02)% with RSD=1.92% (n=5) under the optimized conditions, which was in good agreement with predicted yield. Antioxidant assays suggested that the extracted PA had weaker DPPH, hydroxyl and superoxide free-radical-scavenging capabilities than vitamin C at the same concentration of 0.5 mg/mL.

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Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro

Cited by 502 publications

References 34 publications

Antioxidant Activity of the Derivatives of Polysaccharide Extracted from a Chinese Medical Herb (Ramulus mori)

Antioxidant Activity of the Derivatives of Polysaccharide Extracted from a Chinese Medical Herb (Ramulus mori)

Effect of sulfation on the antioxidant properties and in vitro cell proliferation characteristics of polysaccharides isolated from corn bran

Optimization of extraction conditions for phytic acid from rice bran using response surface methodology and its antioxidant effects

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