Effect of sulfation on the antioxidant properties and in vitro cell proliferation characteristics of polysaccharides isolated from corn bran

Abstract

Polysaccharides were extracted from corn bran (CBPs) and then modified by a sulfating reaction to obtain sulfated corn bran polysaccharides (SCBPs). Synthesized sulfated polysaccharides were located in the equatorial bonds of monosaccharide residues. A more orderly and smooth surface of SCBPs was observed. Single factor experiment results showed 2.0 g of aminosulfonic acid, 60 mL of N-dimethylformamide and 2.5 h of reaction time were the optimum conditions for SCBPs preparation. Phenolic content of SCBPs significantly decreased with increasing concentration of aminosulfonic acid, while it increased with increasing DMF concentration and reaction time. SCBPs had higher DPPH, ABTS and iron chelating capacity under the optimum conditions. SCBPs significantly inhibited the proliferation of A549 and HepG2 cell lines, which was associated with the upregulation at the mRNA expression level of pro-apoptotic genes CASP3, CASP8, CASP9 and reduced expression of p53, gene Bcl-2 and gene iNOS.

In vitro assessment of anti-HCV, antioxidant, cytotoxic and hypolipidemic activities of glycoprotein isolated from Spirulina platensis

“…The sulfated polysaccharides were demonstrated stronger antioxidant capacities than de-sulfated polysaccharides [33]. Also, the high degree of sulfation and low molecular weight showed the best antioxidant capacities [34].…”

Exploiting the Amazing Diversity of Natural Source-Derived Polysaccharides: Modern Procedures of Isolation, Engineering, and Optimization of Antiviral Activities

“…Significant research endeavors are directed towards sulfation of polysaccharides [141,142] and both the advantages and limitations have been recognized dependent on the characteristics of the source materials [19]. A selection of typical reagents namely, oleum [7,8,123,143], chlorosulfonic acid [141,[144][145][146][147][148], sulfamic acid [149][150][151][152][153][154], piperidine-N-sulfonic acid [155][156][157] and SO 3pyridine [25,121,122,[158][159][160][161][162] used in these sulfation processes is highlighted in Figure 5. The benefits and drawbacks of the sulfation modification techniques are presented by Table 2.…”