Antioxidant Activity of Enzymatic Extracts from Brown Seaweeds

Heo, Su Jin; Lee, Gi Wan; Song, Chun Bok; Jeon, Yu Jin

doi:10.4490/algae.2003.18.1.071

Cited by 114 publications

(110 citation statements)

References 21 publications

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“…Therefore, several species of seaweeds are used in cosmetics, food, and pharmaceuticals. Many studies have shown that seaweeds have diverse bioactivities such as antioxidant, anticoagulant, anti-inflammation, antibacterial, and anticancer activities (Heo et al 2003, Shin et al 2006, Kim et al 2007, Erbert et al 2012, Ko et al 2012. In particular, Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea.…”

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confidence: 99%

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Kim¹,

Kim²,

Kang³

et al. 2014

ALGAE

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Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. Polyphenols from E. cava have strong anti-inflammatory activity. However, a large number of the by-products from E. cava processing are discarded. In the present study, to utilize these by-products, we assessed the anti-inflammatory activity of the polyphenol-rich fraction (PRF) from E. cava processing by-product (EPB) in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. Four compounds, namely eckol, eckstolonol, dieckol, and phlorofucofuroeckol-A, were isolated and identified from PRF. We found that PRF suppressed the production of nitric oxide (NO), inducible nitric oxide synthase, and cyclooxygenase-2 in the LPS-induced cells. Furthermore, the protective effect of PRF was investigated in vivo in LPS-stimulated inflammation zebrafish model. PRF had a protective effect against LPS-stimulated toxicity in zebrafish embryos. In addition, PRF inhibited LPS-stimulated reactive oxygen species and NO generation. According to the results, PRF isolated from EPB could be used as a beneficial anti-inflammatory agent, instead of discard.Key Words: anti-inflammation; by-product; Ecklonia cava; polyphenol; seaweeds INTRODUCTIONSeaweeds are rich in vitamins, minerals, proteins, polyphenols, and polysaccharides. Therefore, several species of seaweeds are used in cosmetics, food, and pharmaceuticals. Many studies have shown that seaweeds have diverse bioactivities such as antioxidant, anticoagulant, anti-inflammation, antibacterial, and anticancer activities (Heo et al. 2003, Shin et al. 2006, Kim et al. 2007, Erbert et al. 2012, Ko et al. 2012. In particular, Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. E. cava has been identified to contain various natural substances such as carotenoids, fucoidans, and phlorotannins, which have beneficial biological activities including antioxidant, anti-inflammation, anticoagulant, antiviral, and antidiabetic effects (Kang et al. 2010, 2012b, 2013a. Dried forms of E. cava are consumed, but steam heated E. cava products sometimes are manufactured. When steaming the alga, a large amount of by-product is produced and discarded without utilization. Some studies have suggested that fermenting Received April 25, 2014, Accepted June 3, 2014 *Corresponding Author E-mail: youjinj@jejunu.ac.kr Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Preparation of PRF from EPBEPB was kindly provided by Taerim Co., Ltd. (Jeju, Korea) and then was freeze-dried. The dried EPB powder (5 g) was suspended in 500 mL of 80% ethanol and mechanically stirred for 24 h at room temperature. The solution was filtered and the filtrate was concentrated under reduced pressure to give the oily extract. Aft...

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confidence: 99%

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Kim¹,

Kim²,

Kang³

et al. 2014

ALGAE

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“…In order to overcome such barriers, enzymes can be applied to efficiently extract the desired compounds with higher yields. Enzymatic digests of algae have reported significant yields of desired compounds in the previous studies (Heo et al 2003;Siriwardhana et al 2004). The breakdown and releasing of high molecular weight polysaccharides and proteins themselves may contribute to the enhance antioxidative activities (Ramos and Xiong 2002;Ruperez et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The optimum pH of the each reaction mixtures were adjusted with 1 M HCl/NaOH. Optimum pH and temperature conditions for the respective enzymes were similar as described by Heo et al (2003). In this study, food grade enzymes consisting of five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) were used.…”

Section: Preparation Of Enzymatic Digestsmentioning

confidence: 99%

Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis

Lee¹,

Kim²,

Kang³

et al. 2009

ALGAE

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In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry

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“…E. cava has been widely used for production of fucoidan which is well-known as an antitumor, anticoagulant and antithrombin polysaccharide (Takashi et al, 1999;2000). Also, its antioxidant Heo et al, 2003; and cytoprotective (Kang et al, 2005) activities have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Kim¹,

Kim²,

Khosravi³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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A 6-week feeding trial was conducted to evaluate the efficiency of hijiki (Sargassum fusiforme) and ecklonia (Ecklonia cava) as dietary supplements for juvenile olive flounder. Fish growth performance, hematology, innate immunity and disease resistance were examined. A basal experimental diet was used as a control and supplemented with 6% of either S. fusiforme or E. cava and another diet was prepared to contain 0.5% oxytetracycline (designated as Con, Hij-6, Eck-6 and OTC-0.5, respectively). The experiment was conducted in triplicate (25 fish per replicate) and the fish (initial body weight, 18.3±0.04 g) were fed one of the test diets at a rate of 3% body weight twice daily. Significantly higher (P<0.05) antioxidant capacity and polyphenolic compounds concentration were detected in hijiki and ecklonia supplemented diets. At the end of the feeding trial, fish growth performance and feed utilization were not significantly affected by dietary treatments. Significantly higher (P<0.05) hemoglobin level and respiratory burst activity were found in fish fed Hij-6 diet. The fish fed Hij-6 and Eck-6 diets exhibited higher resistance against Edwardsiella tarda challenge compared to those fed Con and OTC-0.5 diets. The findings in this study may show that hijiki and ecklonia can be used as environment friendly substitutes for antibiotics in diets for olive flounder.

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Antioxidant Activity of Enzymatic Extracts from Brown Seaweeds

Cited by 114 publications

References 21 publications

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis

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