Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis

Abstract

In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry

Antioxidant and anti-inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction

“…The hydrogen peroxide scavenging activity was determined according to the method by Siriwardhana et al (2004). The ferrous ion chelating ability was measured according to the method described by Lee et al (2009). Percentage antioxidant activities were calculated using the following equation:…”

Dietary microalgal-fabricated selenium nanoparticles improve Nile tilapia biochemical indices, immune-related gene expression, and intestinal immunity

“…SeNPs Biosynthesis using plants had been investigated in many literature, however, using microalgae to green synthesize SeNPs is more preferable, considering their rapid growth and ability to double their mass faster than higher plants, besides; their capabilities to reduce metal ions [ 17 , 18 ], due to the formation of biomolecular complexes with metal-chelating biomolecules in algal extracts (e.g., polysaccharides, peptides, and pigments) for capping metal nanoparticles [ 19 , 20 ]. In this context, members of the genus Pediastrum (Sphaeropleales, Hydrodictyaceae), unicellular and colonial chlorophytes, are promising microalgae for biotechnological, food, industrial, and pharmaceutical applications [ 21 , 22 ]. The most widely distributed species in eutrophic freshwater and sediments from the Cretaceous of Egypt is Pediastrum boryanum ( P. boryanum, Turpin) [ 23 ].…”

Dietary algal-sourced zinc nanoparticles promote growth performance, intestinal integrity, and immune response of Nile tilapia (Oreochromis niloticus)

“…P. boryanum is safe for in vivo applications, as its microalgal biomass is ranked as “Category 5,” which refers to secure or minimal toxicity [ 23 ]. Thus, it is considered a promising microalga for biotechnological, food, industrial, and pharmaceutical applications [ 23 , 24 ].…”