Antioxidant activity of five Brazilian plants used as traditional medicines and food in Brazil

Santos, Allana Kellen L.; Costa, José G.M.; Menezes, Irwin Rose Alencar de; Cansanção, Isaac F; Santos, Karla K.A.; Matias, Edinardo Fágner Ferreira; Coutinho, Henrique Douglas Melo

doi:10.4103/0973-1296.71789

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…The chemical structure and reductive properties of these compounds make them active molecules in the scavenging of free radicals and in chelation of transition metals [ 7 ]. Secondary metabolites are substances with a generally complex structure playing several roles in the adaptation of plants to the environment; these substances have been attracting interest for their pharmacological and biotechnological properties such as antioxidant [ 8 ], anti-inflammatory [ 9 ], antimutagenic, anticarcinogenic, gastroprotection [ 10 – 12 ], and antimicrobial [ 13 ] ones.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.‐Hill

Pinho

Cruz

Rodrigues

et al. 2016

Oxidative Medicine and Cellular Longevity

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Background. Duguetia furfuracea is popular plant used in popular medicine. Hypothesis/Purpose. This claim evaluated the phytochemical composition of the hydroethanolic extract (HEDF), fractions of Duguetia furfuracea, and antioxidant and antifungal activity. Methods. The chemical profile was carried out by HPLC-DAD. The total phenolic contents and flavonoid components were determined by Folin-Ciocalteu and aluminium chloride reaction. The antioxidant activity was measured by scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and ferric reducing ability of plasma (FRAP) methods. The antifungal activity was determined by microdilution assay. Results. HPLC analysis revealed caffeic acid and rutin as major compounds (HEDF), caffeic acid and quercitrin (Mt-OH fraction), and quercitrin and isoquercitrin (Ac-OEt fraction). The highest levels of phenols and total flavonoids were found for Ac-OEt fraction, and the crude extract showed higher in vitro antioxidant potential. The antifungal activity showed synergic effect with fluconazole and EHDF against C. krusei, fluconazole and Mt-OH against C. krusei and C. tropicalis, and Ac-OE and fluconazole against C. albicans. Conclusion. The highest levels of phenols and total flavonoids were marked with antioxidant effect. This is the first report of bioactivity of the synergic effect of HEDF and fractions. More studies would be required to better clarify its mechanism of synergic action.

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Section: Introductionmentioning

confidence: 99%

Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.‐Hill

Pinho

Cruz

Rodrigues

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…[ 41 ] In addition, antioxidant activities of five Brazilian plants ( Hyptis martiusii, Mentha arvensis, Momordica charantia, Eugenia jambolana , and Eugenia uniflora ) result indicates that the ethanol extract of all the plants containing phenolic compounds possess strong antioxidant activity and are widely distributed in the plant kingdom. [ 42 ]…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits

et al. 2018

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Background:Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti-inflammatory, and chemoprotective properties.Objectives:In this study, purification of the polyphenol-rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro.Materials and Methods:Antioxidant activities in vitro was measured by quantifying its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power and Fe2+-chelating ability. MPE was purified by high-pressure liquid chromatography (HPLC) and analyzed individual polyphenols using liquid chromatography–mass spectrometry (LC-MS)/MS.Results:The total polyphenol content was 147.69 ± 0.02 mg gallic acid equivalents (GAE)/g dried weight (DW) in the extract and 403.55 ± 0.02 mg GAE/g DW in the purified extract. Further identification by HPLC-ultraviolet-visible and LC-MS/MS analysis indicated in MPE, an anthocyanin compound, cyanidin-3-O-glucoside. With regard to in vitro assays, MPE possessed antioxidant effect, especially in Fe2+ chelating ability with an IC50 value of 1.016 mg/mL. The protective effects on mouse red blood cell hemolysis and lipid peroxidation ex vivo were dose and time dependent.Conclusion:It indicates that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.SUMMARY Mulberry fruit is an excellent source of polyphenols, in particular, anthocyanins, which has infinite health benefits. This study determined the predominant anthocyanin, cyanidin-3-glucoside, could possibly be the rationale behind the antioxidant and antihemolytic effect of MPE. Results indicate that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects. Abbreviations used: MPE: Purification of the polyphenol-rich extract from mulberry fruit, LC-MS: Liquid chromatography–mass spectrometry, HPLC: High-pressure liquid chromatography, DPPH: 2,2-diphenyl-1-picrylhydrazyl scavenging activity, RBC: Red blood cell, GAE: Gallic acid equivalent, FeCl2: Ferrous chloride, H2O2: Hydrogen peroxide, EDTA-2Na: Ethylenediaminetetraacetic acid disodium salt, PBS: Phosphate-buffered saline, TCA: Trichloroacetic acid, TBA: 2-thiobarbituric acid, FeSO4: Ferrous sulphate, MDA: Malondialdehyde, VC: Vitamin C, DW: Dried weight.

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“…[24] The cytoprotective property against mercury chloride is directed by the chelation which can maintain the metal in solution, making it unavailable through precipitation or the complex formed. [25]…”

Section: Resultsmentioning

confidence: 99%

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

Júnior¹,

Coutinho²,

Boris³

et al. 2016

Phcog Res

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Introduction:Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride.Materials and Methods:The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8).Results and Discussion:The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 μg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P < 0.0001) and E. coli (significance P < 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification.Conclusions:The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.SUMMARY The hydroalcoholic extract of A. coriacea Mart. presents in vitro cytoprotective activity against the toxic effect of Hg. Abbreviations Used: HPLC-DAD: High-performance liquid chromatography with a diode array detector; MIC: Minimum inhibitory concentration; DMSO: Dimethyl sulfoxide

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Antioxidant activity of five Brazilian plants used as traditional medicines and food in Brazil

Cited by 23 publications

References 32 publications

Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.‐Hill

Phytochemical Composition, Antifungal and Antioxidant Activity of Duguetia furfuracea A. St.‐Hill

Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

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