Antioxidant activity of flavonoids evaluated with myoglobin method

Terashima, Masaaki; Kakuno, Yuri; Kitano, Naruho; Matsuoka, Chiharu; Murase, Mayumi; Togo, Naoko; Watanabe, Ryoko; Matsumura, Saki

doi:10.1007/s00299-011-1163-2

Cited by 29 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TRAP method suggested that the significant antioxidant activity of the crude extract against the 2 metabolites obtained from A. chica might be because of the synergistic effect of the two major flavonoids present in the extract (scutellarein and apigenin), with other minor flavonoids (such as isoscutellarein, 6-hydroxyluteolin, hispidulin, and luteolin) that are known to have antioxidant activity [44]–[47]. The antioxidant activity of crude extract was significant and dose-dependent as revealed by TRAP, DPPH, and β-carotene/linoleic acid tests.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil

et al. 2013

View full text Add to dashboard Cite

Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC–DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin.

show abstract

Section: Resultsmentioning

confidence: 99%

Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Between these are highlighted ferulic acid (79) , carotenoids (80) , tannic acid (81) , DHB (82) , flavonoids (83) .…”

Section: Ligands Effects On Fenton Reactionmentioning

confidence: 99%

Fenton Reaction Driven by Iron Ligands

Salgado

Melín

Contreras

et al. 2013

J. Chil. Chem. Soc.

107

View full text Add to dashboard Cite

One of the most important sources of reactive oxygen species (ROS) in biological systems is the Fenton reaction. In this, the Fe 2+ or Fe 3+ reacts with H 2 O 2 to produce ROS as the hydroxyl radical (•OH), superoxide radical (O 2 •-) and singlet oxygen ( 1 O 2 ). The main ROS, responsible for the high oxidizing power of the Fenton reaction, is not clear. Some authors claim that the principal reactive species is •OH, while others propose a ferryl specie (Fe 4+ or [FeO] 2+ ) (1,2) . Recently, have been proposed that the kind of reaction species produced depends mainly of pH and the iron composition of the coordination sphere. This is highlighted for Fe 3+ , because in mono and (some) bis-complexes Fe 3+ is reduced to Fe 2+ and there are some positions occupied by water or hydroxide ligands, readily to be exchanged by H 2 O 2 . On the other hand, in tris-complexes there are not any positions occupied by water or hydroxide, avoiding the formation of peroxo-complexes, necessary for Fenton or Fenton like reaction.The 1,2-dihydroxybenzenes (DHBs) have been described as modulators of Fenton reaction. The DHBs driven Fenton reaction have been used for environmental applications as an advanced oxidation process. Furthermore, these systems participate in different biological process, as the wood biodegradation by fungi and oxidative stress in neurodegenerative diseases.In this review, the effect of 1,2-dihydroxybenzenes on the activated species production by the Fenton and Fenton like reaction will be discussed and its participation in different systems.

show abstract

“…[19], which used lower concentrations of AAPH (13.9 mM) and higher reaction temperature (47 • C) in order to enhance the generation rate of peroxyl radicals to oxidize protein. Moreover, as the intrinsic absorption of AAPH was not considered, a residual absorbance value was obtained at the end of the reaction [20].…”

Section: Evaluation Of Analytical Conditions Of Myoglobin Microplate mentioning

confidence: 99%

“…Myoglobin denaturation due to peroxidation is reflected in a decrease of absorbance at 409 nm (Soret band) owing to the exposure of the heme group, buried in a hydrophobic pocket within the protein's interior, to the polar aqueous solvent [18]. This probe was further applied toward different reactive species [19] as well as to determine antioxidant properties of flavonoids [20], leafy vegetables and beans [21] and commercially available miso [22]. However, for peroxyl radical scavenging capacity, samples were pre-incubated at 47 • C and the myoglobin protective ratios were calculated from the data after 30 min of reaction, not considering the kinetic information provided by the area under the curve.…”

Section: Introductionmentioning

confidence: 99%