Antioxidant Activity of Flaxseed (Linum usitatissimum L.) shell and Analysis of Its Polyphenol Contents by LC-MS/MS

Han, Hatica; Yilmaz, Hasibe; Gülçin, İlhami

doi:10.25135/rnp.46.17.09.155

Cited by 77 publications

(54 citation statements)

References 15 publications

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“…The CUPRAC assay is rapid, stable, cheap, selective, and suitable. [97] DPPH· and ABTS ·+ radical scavenging assays are the most putative and applicable methods to determine of the antioxidant capacities and radical scavenging effects of beverages, foods and plants. The DPPH· scavenging ability is the most common and oldest method for determining the antioxidant activities.…”

Section: Antioxidant Resultsmentioning

confidence: 99%

Anticholinergic, antidiabetic and antioxidant activities of cinnamon ( cinnamomum verum ) bark extracts: polyphenol contents analysis by LC-MS/MS

Gülçın

Kaya

Gören

et al. 2019

International Journal of Food Properties

131

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Ethanolic (EEC) and aqueous (WEC) extracts of cinnamon (Cinnamomum verum)were evaluated for their antioxidant profiles by eight distinguished bioanalytical antioxidant methods. Their inhibitory effects were tested against some enzymes including acetylcholinesterase, butyrylcholinesterase, α-glycosidase and αamylase, which linked to different diseases. Additionally, the antioxidant properties were determined and polyphenolic compositions of the both extracts were evaluated by LC-MS/MS analysis. According to the LC-MS/MS experiments, thirteen compounds were found in WEC and EEC. Also, p-hydroxybenzoic acid (321.1 mg/kg extract), p-coumaric acid (291.4 mg/kg extract), and pyrogallol (142.4 mg/kg extract) were found to be the most abundant ingredients in the WEC. On the other hand, pyrogallol (264.3 mg/kg extract), ferulic acid (224.7 mg/ kg extract) and p-coumaric acid (170.2 mg/kg extract) were found as the most plentiful chemicals in the EEC. For the estimation of the antioxidant capacities of the both extracts (WEC and EEC), DPPH· and ABTS •+ scavenging activities, as well as Fe 3+ -Fe 2+ , and Cu 2+ -Cu + reducing assays were studied. The IC 50 values of the WEC and EEC indicated that they were potent effective DPPH· (21. 25 and 15.71 μg/mL) and ABTS •+ (6.52 and 5.79 μg/mL) scavengers, as well as AChE (221.33 and 110.26 μg/mL), BChE (461.69 and 94.93 μg/mL), α-glycosidase (206.86 and 220.00 μg/mL) and α-amylase (189.86 and 200.86 μg/mL) inhibitors. As a conclusion, both EEC and WEC had rich phenolic contents and demonstrated effective anticholinergic, antidiabetic and antioxidant effects. ARTICLE HISTORY

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Section: Antioxidant Resultsmentioning

confidence: 99%

Anticholinergic, antidiabetic and antioxidant activities of cinnamon ( cinnamomum verum ) bark extracts: polyphenol contents analysis by LC-MS/MS

Gülçın

Kaya

Gören

et al. 2019

International Journal of Food Properties

131

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“…Our result also exhibits the presence of sinapic and p-coumaric acid in LUE; however, the extract was rich in gallic acid ferulic, and vanillic acid. Han et al (2018) reported the ability of the ethanolic and aqueous extract of flaxseed shell to scavenge DPPH radical with 49.50 and 53.30 μg/mL that was less than LUE activity. On the other hand, the ability of their extract to chelate ferrous ions 9.24 and 8.88 μg/mL was more than the extract of this research.…”

Section: Discussionmentioning

confidence: 99%

HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways

et al. 2020

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Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC50 = 19.3 ± 1.1 µg/mL. LUE chelated ferrous ions with IC50 = 121.1 ± 1.3 µg/mL. LUE showed anti-proliferative activity on LNCaP cells with the IC50 values of 8.3, 6.3, and 5.4 μg/mL after 24, 48, and 72 h treatment. LUE also increased cell mortality by inducing apoptosis (15.3–29.8%). The real-time qPCR results exhibited an increase in gene expression of Bax and caspase-3. Our in vitro study demonstrates that L. usitatissimum can be considered as an effective agent to inhibit the growth and invasion the human prostate cancer cells.

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“…Fruits are supposed to contain diversified bioactive compounds, which demonstrate biological activity in terms of antioxidant, antimicrobial and anticancer properties. These chemical classes have considerable disease preventing and health promoting effects on human body (Han et al, 2018).…”

Section: Estimation Of Total Phenolic Contents Flavonoids Contents Amentioning

confidence: 99%

Physico-chemical and Nutraceutical Characterization of Selected Indigenous Guava (Psidium guajava L.) Cultivars

et al. 2021

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In order to ascertain physicochemical and nutraceutical attributes, indigenous guava (Psidium guajava L.) cultivars were comprehensively characterized. Eight cultivars namely Gola, Chota Gola, Surahi, Choti Surahi, Sufaida, Sdabahar, Lal Badshah and Karela were selected due to their climatic adaptability and commercial suitability. All the cultivars showed significant variations in terms of their studied quality attributes. Amongst physical characteristics, Gola exhibited highest (79.9 mm 3) GMD with lowest (50.3 mm 3) was estimated in Choti Surahi. Insignificant varietal differences were observed in most of the proximate parameters as well as in mineral contents. Nutraceutical estimations showed significant variation in ascorbic acid (222.26-289.43 mg/100 g), total phenolic contents (94.06-190.64 mg GAE/100 g), total flavonoid contents (81.30-154.19 mg QE/100 g) and radical scavenging activity (27.70-78.15%) in the selected cultivars. A highly significant correlation (R 2 = 0.9970 p < 0.05) was observed between ascorbic acid and radical scavenging activity. In sensory evaluation, Gola received over all maximum scores (8.8) amongst its counterparts. Processed data were then analyzed using Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA). The combination of PCA and HCA yielded in a sufficient discrimination of the examined guava cultivars. In PCA analysis, first two PCA components explained 65.98% of the total variation. Dendrogram successfully classified the tested cultivars into three major groups featuring dissimilarities amongst the cultivars. Research outcome will provide baseline for the farmers, researchers, exporters and other stalk holders to realize the ultimate potential of indigenous guava cultivars for their appropriate commercial utilization.

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Antioxidant Activity of Flaxseed (Linum usitatissimum L.) shell and Analysis of Its Polyphenol Contents by LC-MS/MS

Cited by 77 publications

References 15 publications

Anticholinergic, antidiabetic and antioxidant activities of cinnamon ( cinnamomum verum ) bark extracts: polyphenol contents analysis by LC-MS/MS

Anticholinergic, antidiabetic and antioxidant activities of cinnamon ( cinnamomum verum ) bark extracts: polyphenol contents analysis by LC-MS/MS

HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways

Physico-chemical and Nutraceutical Characterization of Selected Indigenous Guava (Psidium guajava L.) Cultivars

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