Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro

Jayaprakasha, G.K.; Singh, Ravendra; Sakariah, K.K.

doi:10.1016/s0308-8146(00)00298-3

Cited by 1,029 publications

(708 citation statements)

References 17 publications

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“…As mentioned by (Jayaprakasha et al 2001) the bleaching pathway of β-carotene is a free radical phenomenon which is resulting from the hydroperoxidation of linoleic acid. In the absence of antioxidant, β-carotene will be under rapid discoloration as a result of losing their double bonds by oxidation.…”

Section: Discussionmentioning

confidence: 99%

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

et al. 2013

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The antioxidant components of cocoa powder, which is rich in polyphenols, were isolated using column chromatography and high performance liquid chromatography. Polyphenolic compounds were then characterized by high-performance liquid chromatography/Ultraviolet and electronspray ionization-tandem mass spectrometry (HPLC-UV-/ESI-MS-MS). As a result, five phenolic compounds were detected. In this study we also investigated scavenging or the total antioxidant capacity (%) of cocoa polyphenol (CP) fractionated from cocoa powder extract. 114.0 mg/g of gallic acid -equivalent phenolics and 94.3 mg/ g catechin-equivalent flavonoids were quantified in this extract. Their free radical-scavenging activity was assessed by 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) assay, β-carotene bleaching test, and xanthine oxidase inhibitory activity (OX). Total antioxidant capacity (TAC) was further assessed against the myoglobin-induced oxidation of 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (ABTS) and expressed as Trolox equivalent. A high correlation between TAC and phenolic contents indicated that phenolic compounds from cocoa were a major contributor of antioxidant activity (0.967≤r≤1.00). CP extract had significantly (P <0.05) potential antioxidant activities with various concentrations. These results suggest that Polyphenols-rich cocoa extract possess prominent medical properties and can be exploited as natural drug to treat free radical associated diseases.

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Section: Discussionmentioning

confidence: 99%

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

et al. 2013

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“…The increase in the absorbance indicates increase in the antioxidant activity and in turn, the increase in absorbance of the reaction mixture indicates the reducing power of the samples (Jayaprakash et al 2001). There is a direct correlation between antioxidant activity and reducing power of plant extract.…”

Section: Dpph Scavenging Abilitymentioning

confidence: 99%

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

Harsha

Anilakumar

2012

J Food Sci Technol

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Coriandrum sativum L. (coriander), an everyday spice in the Indian kitchen is known to add flavor to the cuisine. It is an annual herb belonging to the Apiaceae (Umbellifera) family. The hydro-alcohol extract of Coriandrum sativum L. at the dose of 1 mg/ml was subjected to a series of in vitro assays viz. 2, 2′-diphenyl-1-picrylhydrazyl, lipid peroxidation by thiobarbituric acid, reducing power and nitric oxide (NO) radical scavenging in order to study its antioxidant efficacy in detail. The amount of flavonoids in 70% ethanol extract was found to be 44.5 μg and that of the total phenols was 133.74 μg gallic acid equivalents per mg extract. The extracts of the leaves showed metal chelating power, with IC 50 values, 368.12 μg/ ml where as that of standard EDTA was 26.7 μg/ml. The IC 50 values for 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid radical scavenging was 222 μg/ml where as that of standard ascorbic acid was 22.6 μg/ml. The NO scavenging activity of the extract of the leaves showed IC 50 value of 815.6 μg/ml; at the same time the standard BHA had 49.1 μg/ml. All the plant extracts provided DNA damage protection; however, the protection provided at the dose of 8 μg/ml was comparable to that of standard gallic acid. The Coriandrum sativum leaf extract was able to prevent in vitro lipid peroxidation with IC 50 values; 589.6 μg/ml where as that of standard BHA was 16.3 μg/ml. Our results also showed significant ferric reducing power indicating the hydrogen donating ability of the extract. This study indicated the potential of the leaf extract as a source of natural antioxidants or nutraceuticals that could be of use in food industry with potential application to reduce oxidative stress in living system.

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“…The concentration of phenolic compounds in the extracts was determined, as described by Jayaprakasha et al (2001) and results were expressed as tannic acids equivalents (TAE). The extracts were dissolved in a mixture of methanol and water (6:4 v/v).…”

Section: Determination Of Total Phenolicsmentioning

confidence: 99%

“…The reducing power of the test samples was determined, as described by Jayaprakasha et al (2001). Different concentrations of TSCE in 1 ml MeOH were mixed with 2.5 ml of phosphate buffer (0.2 M, pH 6.6) and 2.5 ml of 1 % potassium ferricyanide.…”

Section: Reducing Power Assaymentioning

confidence: 99%

Antioxidant activities of tamarind (Tamarindus Indica) seed coat extracts using in vitro and in vivo models

2014

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Tamarindus indica seed coat was extracted with methanol, acetone and water and screened for DPPH radical scavenging activities. Methanol extract showed higher activity than other extracts. Treatment of albino rats (Wistar strain) with CCl 4 at 1.25 mL/kg of body weight decreased superoxide dismutase (55 %), catalase (73 %) and peroxidase (78 %), while lipid peroxidation increased nearly 2.5 fold in liver. Pretreatment of rats with methanol extract of T. Indica seed coat (TSCE) at 50 mg/kg (as tannic acid equivalents) followed by CCl 4 treatment, caused restoration of superoxide dismutase, catalase and lipid peroxidation to values close to control while peroxidase was restored to 67 % of the control. Histopathological studies of liver of different groups supported the protective effects of TSCE by restoring the hepatic architecture. These studies could be further extended to exploit its possible application for the preservation of food products as well as a health supplement and neutraceutical.

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Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro

Cited by 1,029 publications

References 17 publications

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

In vitro free radical scavenging and DNA damage protective property of Coriandrum sativum L. leaves extract

Antioxidant activities of tamarind (Tamarindus Indica) seed coat extracts using in vitro and in vivo models

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