Antioxidant activity of herbaceous plant extracts protect against hydrogenperoxide-induced DNA damage in human lymphocytes

Lin, Kuan‐Hung; Yang, Yuchen; Yang, Chi-Ming; Huang, Mengyuan; Lo, Hsiao-Feng; Liu, Kuang-Chuan; Lin, Hwei-Shen; Chao, Pi-Yu

doi:10.1186/1756-0500-6-490

Cited by 53 publications

(34 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, comet scoring was performed using a scale of 0 (no damage) to 3 (most damage) in visual analysis in the comet assay between 10 and 30 µM H 2 O 2 damaging concentrations. A significant increase was induced in DNA damage with increasing the concentration of H 2 O 2 (10, and 20 µM; P < 0.001 for all; Figure 2 (18,39,40) and incubation time of H 2 O 2 (5 Min), which were compatible with previous literature (18,41,42). In this study, Primula vulgaris extract, rich in phenolic compounds, was investigated for its protective effect on H 2 O 2 -induced oxidative DNA damage in foreskin fibroblast cells.…”

Section: Discussionsupporting

confidence: 90%

Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells

Ozkan

Aliyazıcıoğlu

Demir

et al. 2016

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

Background: Primula vulgaris has been used in traditional treatment, and its biological functions are attributed to its polyphenolics content. Objectives:The aim of this study was to determine the phenolics composition and the antioxidant activity of water extract of P. vulgaris (WEP) and to determine its probable preventive effects against H2O2-induced DNA damage in human fibroblast cells. Methods: The total polyphenolic content (TPC), ferric reducing antioxidant power (FRAP), and radical scavenging activity of WEP were determined using spectrophotometric methods. Phenolic compounds and antigenotoxic effects of WEP were evaluated using HPLC and comet assay, respectively. Results:The TPC and FRAP values of WEP were 15.023 ± 0.84 mg gallic acid and 82.63 ± 0.31 µM trolox per g sample, respectively. ρ-coumaric acid and rutin were detected as major phenolics. Moreover, WEP reduced H2O2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to the positive controls (only 20 µM H2O2 treatment). Conclusions:Primula vulgaris can be used in food, cosmetics, and drug industries because of its antioxidant and antigenotoxic activities.

show abstract

Section: Discussionsupporting

confidence: 90%

Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells

Ozkan

Aliyazıcıoğlu

Demir

et al. 2016

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

show abstract

“…The screening and characterization of antioxidants derived from natural sources has gained much attention and efforts have been put into identifying compounds as suitable antioxidants to replace synthetic ones. The search for phytochemicals with potent antioxidant continues to be of great importance in the search for remedies against free radical-mediated diseases, prevention of oxidative reactions in foods, protection against DNA damage and carcinogenesis and possible substances with wide range of pharmacological activities such as anti-inflammatory, antibacterial and antifungal properties (Lin et al, 2013).…”

Section: Dpph Antioxidant Activity Of the Plant Extractsmentioning

confidence: 99%

Chemical Composition, Antioxidant and Antimicrobial Potentials of Icacina trichantha Oliv. Leaf Extracts

Otun¹,

Onikosi²,

Ajiboye³

et al. 2015

Research J. of Phytochemistry

View full text Add to dashboard Cite

The chemical compositions of the leaf extracts of Icacina trichantha plant extracted successively with n-hexane, ethyl acetate and ethanol was analyzed by GC/GCMS. The preliminary phytochemical screening conducted on the crude leaf extracts revealed the presence of tannins, flavonoids, phenols and glycosides which are known to support the bioactive activities of the plant in folk medicine. The antibacterial activity of Icacina trichantha of the three extracts against gram-negative bacteria namely; Escherichia coli, Pseudomonas aeruginosa and Klebsiella oxytoca was evaluated in the present research study. It was found that the hexane and ethyl acetate extracts are more effective than that of ethanol against the three bacteria strains. The free radical scavenging activity of the extracts by spectrophotometric assay on the reduction of 1,1-diphenyl-2picrylhydrazyl (DPPH) was also examined. The n-hexane extract possessed the highest antioxidant activity with half maximal inhibitory concentration (IC 50) of 0.21 µg mLG 1 followed by ethyl acetate extract; 3.917 µg mLG 1 and ethanol; 4.812 µg mLG 1. We hereby report for the first time the major compounds from the hexane, ethyl acetate and ethanol extracts of Icacina trichantha to be stearolic acid (30.74%), oleic acid (36.04%) and erucic acid (29.01%) respectively; all of which contribute individually and or synergistically to the biological activities reported in this study.

show abstract

“…Therefore, it is important to use different types of solvent extractions and various methods to 0.410* 0.485** 0.629** 0.576** **Correlation is significant at p < 0.01; *Correlation is significant at p < 0.05. TPC, total phenolic contents; TFC, total flavonoid contents; DDPH, 2,2-Diphenyl-1-picrylhydrazyl; ABTS, 2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid; FRAP, ferric reducing antioxidant power; PAP, phosphomolybdenum antioxidative power; BCB, β-carotene bleaching assay Table 4: Phytochemicals identified in the leaves and stems of M. micrantha extracted using ethyl acetate evaluate the antioxidant capacities of plant extracts to avoid a misleading interpretation (Lin et al, 2013). From our data, the ethyl acetate stems (EAS) extract stands out with efficient antioxidant capacities (Table 2), which significantly correlated with the phenolic and flavonoid contents (Table 3).…”

Section: Discussionmentioning

confidence: 99%

From Weed to Medicinal Plant: Antioxidant Capacities and Phytochemicals of Various Extracts of Mikania micrantha

Ishak¹

2018

IJAB

View full text Add to dashboard Cite

Mikania micrantha is commonly consumed as traditional medicine in some countries, including Malaysia. Little is known about the antioxidant properties and phytochemicals of M. micrantha. This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacities of the leaves and stems of M. micrantha of hot water, cold water, 70% ethanol, ethyl acetate, and hexane extracts. Folin-Ciocalteu and aluminium chloride colorimetric assays were used to determine the TPC and TFC, respectively. The antioxidant capacities were determined using rapid, inexpensive and small-scale microplate of five different antioxidant assays. Gas chromatography-mass spectrometry (GC-MS) was used to chemically profile and characterize the phytochemicals. In comparison with different solvents, the ethyl acetate stems (EAS) and leaves (EAL) extracts of M. micrantha had the significantly greatest TPC (141 ± 0.51 mg gallic acid equivalent/g) and TFC (70.1 ± 0.92 catechin equivalent/g), respectively. Moreover, EAS extract had the significantly greatest antioxidant capacities using DPPH (EC50 = 324 ± 61.4 μg/mL), ABTS (0.53 ± 0.01 mmol trolox equivalent/g), FRAP (1.28 ± 0.05 mmol Fe 2+ /g), phosphomolybdenum antioxidative power (219 ± 7.03 mg ascorbic acid equivalent/g), and β-carotene bleaching (108 ± 2.23%) assays. GC-MS analysis of EAS showed the presence of sesquiterpenes (30.46%), phenol (16.38%), and alkane hydrocarbons (10.45%), which may contribute to its antioxidant capacities. These findings suggest the stems extract of M. micrantha using ethyl acetate as the potential source of natural antioxidant agents and its utilization to prevent oxidative damage-related diseases could be further explored.

show abstract

Antioxidant activity of herbaceous plant extracts protect against hydrogenperoxide-induced DNA damage in human lymphocytes

Cited by 53 publications

References 40 publications

Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells

Phenolic Characterisation and Antioxidant Activity of Primula vulgaris and Its Antigenotoxic Effect on Fibroblast Cells

Chemical Composition, Antioxidant and Antimicrobial Potentials of Icacina trichantha Oliv. Leaf Extracts

From Weed to Medicinal Plant: Antioxidant Capacities and Phytochemicals of Various Extracts of Mikania micrantha

Contact Info

Product

Resources

About