Antioxidant activity of mangrove-derived marine thraustochytrids

Abstract

Antioxidants are compounds that inhibit the reaction caused by free radicals thereby preventing or delaying damage to the cells and tissues. Protection against free radicals can be enhanced by taking sufficient amounts of exogenous antioxidants. The present study evaluated the antioxidant activity of the mangrove-derived thraustochytrids for their potential health benefits. Thirteen thraustochytrid strains were isolated from mangrove leaf litter, extracted them separately in methanol and tested for their antioxidant activity by using six different assays: DPPH radical scavenging, total phenol content, hydrogen peroxide radical inhibition, nitric oxide radical assay and total reducing power activity. The antioxidant activity was found significant between the thraustochytrid strains and between concentrations of their extracts (P< 0.05). The activity also increased with concentration of the extracts. The strain TSKK1 exhibited highest (78.95±1.29) antioxidant activity and TSSK5 the lowest antioxidant activity (72.52±2.03). This study proved the potent free radical scavenging and antioxidant activity of thraustochytrids.

Thraustochytrids of Mangrove Habitats from Andaman Islands: Species Diversity, PUFA Profiles and Biotechnological Potential

“…The results are shown in Figure 8 . The DPPH free-radical scavenging assay was significant between thraustochytrids and the scavenging activity also increased with an increase in the concentrations of their extracts ( p < 0.05) and this is in agreement with an earlier report on other species of thraustochytrids [ 26 , 69 ]. The activity was visually noticeable as the color changed from purple to yellow.…”

“…ANVKK-03 and ANVKK-10 showed high inhibition against human and fish pathogens of S. aureus (18.69 ± 1.2 mm) and V. parahaemolyticus (18.31 ± 1.0 mm), whereas ANVKK-07 and ANVKK-01 exhibited low inhibition against human pathogens of E. coli (4.32 ± 0.5 mm), followed by V. cholera (4.34 ± 0.2 mm), and ANVKK-02 and ANVKK-12 also displayed minimum inhibition against fish pathogens of V. alginolyticus (4.15 ± 0.4 mm), followed by V. harveyi (4.66 ± 1.12 mm) ( Table 4 ). This is supported by earlier works where thraustochytrid-derived fatty acids have antibacterial activity besides [ 25 ] antioxidant [ 26 ], antiviral [ 64 ], and anticancer [ 28 ] properties.…”

“…Notably, Schizochytrium strains were isolated in Guptapara, which is a pristine mangrove habitat in the western part of the south Andaman. The presence of thraustochytrids have been reported only in limited areas of Indian mangrove regions such as Goa [ 34 ], Kerala [ 35 ], Mumbai [ 36 ], and Tamil Nadu [ 19 , 22 , 25 , 26 ]. Thraustochytrids for many mangrove habitats of global mangroves have still been poorly studied.…”

“…The thraustochytrids are a potential source of natural antioxidants and free radical scavengers due to the presence of omega-3 fatty acids [ 26 ]. The DPPH assay is an important tool for assessing antioxidant activity.…”

“…Thraustochytrids constitute a major component of the microbiota in mangrove ecosystems [ 22 ]. They are known as a promising microbial source for the commercial production of omega-3 polyunsaturated fatty acids (PUFA) such as docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), antimicrobial, antioxidant, and nanoparticle properties for their application in human health, aquaculture, and nutraceutical sectors [ 22 , 23 , 24 , 25 , 26 ]. DHA and EPA are also known to have positive results in the prevention of cardiovascular, neurological, and anticancer diseases [ 27 , 28 ].…”