Antioxidant Activity of Methanol Fractions Stem Bark of Kayu Sarampa (Xylocarpus moluccensis (Lam.) M. Roen))

Abstract

Introduction: Methanol extract of X. moluccensis was found to be significantly effective in scavenging DPPH method. Therefore, this research is a follow-up research study from Budiarso et al (2020).. The methanol extract was then fractionated and tested for antioxidant activity. Objective: To assess antioxidants activity of methanolic fractions from stem bark of Kayu Sarampa. Method: The Stem bark was extracted with Reflux method using hexane, ethyl acetate, and methanol as solvent. The methanolic extract was fractionated using a chromatographic column were subjected to the antioxidant activity assay by the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and the ferric-reducing antioxidant power (FRAP) method. Results: F3 Fractions IC 50 of X. moluccensis exhibits the highest DPPH scavenging activity compared with F2, F3, ascorbic acis as control positif, F5, and F4, wich are 4. 64, 6.79, 9.69, 10.49, and 227.44 respectively and Ferric reducing power from methanolic fraction of X. moluccensis stembark F3 exhibits higher antioxidant power compared to F2, F1, F5, ascorbic acid and F4, respectively which are 667.8 μmol/gr, 607.8 μmol/gr, and 573.8 340.48 and 309.8 μmol/gr, respectively

In Vitro and In Silico Screening Analysis of Artabotrys sumatranus Leaf and Twig Extracts for α-Glucosidase Inhibition Activity and Its Relationship with Antioxidant Activity

“…Ascorbic acid was used as the positive control, which was treated in the same way as the sample (replacing the sample with ascorbic acid). The result of the FRAP method can be reported in Fe 2+ mol/g extract (similar to the units used in [36,37]), which can be computed using the following formula:…”