Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus

Benhammou, Nabila; Bekkara, Fawzia Atik; Panovska, Tatjana Kadifkova

doi:10.1016/j.crci.2009.02.004

Cited by 100 publications

(72 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cell viability remained above 85% at relatively high concentrations, indicating that LAECs and Cs1 were non-toxic to human leukocytes. Antioxidant tests were performed using a DPPH assay; this is a widely accepted method for evaluation of in vitro antioxidant activity of extracts and is based on the capacity of antioxidant compounds present in the sample to capture DPPH radicals (Benhammou, Bekkara, Panovska, 2009;Ayala-Zavala et al, 2012;Mohsin, Mahadevan, Kurup, 2014;Wan-Ibrahim, Sidik, Kuppusamya, 2010). The results showed that LAECs and Cs1 inhibited ≥ 50% of radicals at concentrations of 10 µg/mL and 50 µg/ mL, showing that both samples appear to protect the studied biological matrix, since the concentrations tested were able to neutralize the radical DPPH.…”

Section: Discussionmentioning

confidence: 99%

“…Under normal conditions, reactive oxygen species (ROS) are an essential part of such processes, and the most common ROS include the superoxide anion (O 2 −• ), hydrogen peroxide (H 2 O 2 ), peroxyl radicals (ROO • ), and reactive hydroxyl radical (HO • ) (Benhammou, Bekkara, Panovska, 2009;Gaur et al, 2009). The imbalance between free radical production and endogenous detoxification systems (predominantly non-enzymatic and enzymatic antioxidants) can lead to oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…The imbalance between free radical production and endogenous detoxification systems (predominantly non-enzymatic and enzymatic antioxidants) can lead to oxidative stress. Damage occurs mainly through the interaction of these radical species and macromolecules such as lipids, proteins, and deoxyribonucleic acid (DNA) (Benhammou, Bekkara, Panovska, 2009). The interaction of free radicals with DNA can lead to mutations and may therefore promote carcinogenesis, and accentuate the processes involved in the development of atherosclerosis, arthritis, and ischemia.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Malheiros

Silva

Hofmann

et al. 2017

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

The bark tea of Ceiba speciosa, a tropical tree of the Malvaceae family, is used in the Northwestern Region of Rio Grande do Sul state, Brazil, to reduce blood cholesterol levels. However, there are no scientific data on the efficacy and safety of this plant. The aim of the present study was to evaluate the in vitro antioxidant and toxic potential of bark extracts of C. speciosa. We performed a preliminary phytochemical analysis by high-performance liquid chromatography-diode array detection (HPLC-DAD) and evaluated the oxidative damage to proteins and lipids, the radical scavenging effect, and genotoxicity of the lyophilized aqueous extract (LAECs) and the precipitate obtained from the raw ethanol extract (Cs1). The phytochemical profile demonstrated the presence of phenolic and flavonoid compounds. The LAECs and Cs1 prevented damage to lipids and proteins at concentrations of 50 and 10 µg/mL. They also showed a scavenging effect on 2,2-diphenyl-1-pricril-hydrazyl (DPPH) radicals in a concentration-dependent manner. Furthermore, no genotoxic effect was observed at concentrations of 10, 5 and 2 µg/mL in the Comet assay. The present study is the first evaluation regarding the characterization of C. speciosa and its safety, and the results demonstrate its antioxidant potential and suggest that its therapeutic use may be relatively safe.Uniterms: Ceiba speciose/phytochemistry. Ceiba speciose/antioxidant activity. Phenolic content. Comet assay. DPPH scavenging capacity. Natural medicine. Safety.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Malheiros

Silva

Hofmann

et al. 2017

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…For flavonoids extraction, the method of Benhammou et al (2009) was applied; thus, 20 g of the dry powder of L. halimifolium Mill leaves was extracted in 200 mL of methanol for 24h at room temperature. The extracts were concentrated in a rotary evaporator at 60°C under a reduced pressure.…”

Section: Extraction Proceduresmentioning

confidence: 99%

Inhibition of aflatoxin B1 production of Aspergillus flavus isolated from peanut seeds using Lycium halimifolium Mill leaves fractions

Mosbah¹,

Milet²,

Ali³

et al. 2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The aim of this study is to explore the impact of Lycium halimifolium Mill leaves extracts on mycotoxin B 1 production in vitro, as well as the inhibitor effect of the aflatoxin B 1 synthesis by Aspergillus flavus strain. The strain was isolated, identified (gi |146746162 | EF409803.1.) and positively tested for the production of Aflatoxin B 1 . The antimycotoxinogenesis effect was investigated using L. halimifolium Mill leaves consisting of butanol and ethyl acetate fractions. Indeed, the fractions were tested to stop the secretion of aflatoxin B 1 by A. flavus in submerged culture (YES), simultaneously. The growth of the strain was evaluated as biomass response. The antimycotoxinogenesis activity showed that the two fractions: ethyl acetate and butanol of L. halimifolium Mill leaves were able to inhibit significantly the synthesis of mycotoxin AFB 1 . Thus, the secretion inhibition percentage was calculated in comparison with the control, using HPLC-PDA technique, and estimated at 96.83% for butanolic fraction and 94.99% for ethyl acetate fraction. To verify its correlation with biomass, the growth inhibition percentage was also estimated. The results showed that the dry weight concentration under butanolic and ethyl acetate fractions was 1.6 and 2 g/l, respectively, corresponding to 65.22 and 56.53% of inhibition growth percentage.

show abstract

“…After filtration through filter paper, the resulting solution was evaporated under vacuum at 60°CbyBuchi RotavaporR-200dry. The residue was then dissolved in 3mL of methanol 13 .…”

Section: Preparation Of Methanolic Extractsmentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

Seven parts of Pistacia atlantica were collected from Tlemcen in Algeria (fruits, leaves, buds, stems, roots internal and external trunk barks). Tannin content was determined and antioxidant activity of tannic extracts were measured using different assays: Total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power (FRAP) and β-carotene bleaching assay The results showed that the highest tannin contents were observed in roots(49.789±0.391 mg CE/g DM) and internal trunk barks (49.095±0.306 mg CE/g DM).Tannins extracted from leaves, roots and external trunk barks showed very good DPPH radical scavenging activity (IC50 of 0.124±0.001, 0.304±0.001 and 0.440±0.001 mg/mL), ferric reducing power (0.021±0.001, 0.154±0.001 and 0.170±0.005 mg/mL) and β-carotene bleaching inhibition (0.313±0.009, 0.094±0.006 and 0.131±0.002 mg/mL), respectively. Therefore, the results suggest that this plant with all its studied parts might be considered as a potential candidate of possible health-promoting functional foods.

show abstract

Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus

Cited by 100 publications

References 30 publications

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Preliminary in vitro assessment of the potential toxicity and antioxidant activity of Ceiba speciosa (A. St.-Hill) Ravenna (Paineira)

Inhibition of aflatoxin B1 production of Aspergillus flavus isolated from peanut seeds using Lycium halimifolium Mill leaves fractions

Untitled

Contact Info

Product

Resources

About