Antioxidant Activity of Pepsin Hydrolysate Derived from Edible Hippocampus abdominalis in vitro and in Zebrafish Models

Abstract

Seahorse Hippocampus abdominalis a marine teleost fish, has long been used as one of the essential materials in traditional Chinese medicine. However, the uses of seahorse have been limited due to its high cost, despite its beneficial biological activities. Seahorse has not been widely explored for its biofunctional properties and active components.In the present study, the enzymatic hydrolysates of seahorse were prepared by using two digestive enzymes (trypsin and pepsin) and five food grade enzymes (neutrase, protamex, alcalase, kojizyme, and flavourzyme). The enzymatic hydrolysates indicated higher hydrolysis yields than its water extract. Among them, the distilled water-pepsin hydrolysate (DP) which was obtained by distilled water extraction followed by pepsin hydrolysis, showed the highest yield and protein content as well as the highest alkyl radical scavenging activity. Also, it provided protective effects against oxidative stress induced by AAPH in vero cell and zebrafish. Further fractionation based on the molecular weight was carried out to identify it's active components, and < 5 kDa (less than 5 kDa) molecular weight fraction was confirmed to have the highest antioxidant activity. In conclusion, this study suggests that DP of seahorse has antioxidant properties, and might be a novel and useful material from the marine origin for healthy functional foods and cosmetics.

Free radical scavenging activity of the peptide from the Alcalase hydrolysate of the edible aquacultural seahorse ( Hippocampus abdominalis )

“…HPH‐III possessed the highest peroxyl radical scavenging activity (the half maximal inhibitory concentration; IC 50 of 0.46 mg/ml) (Figure ). Similar findings were reported by Kim et al (), in which they found that the seahorse pepsin protein hydrolysate with a molecular weight greater than 5 kDa had a higher antioxidant activity (IC 50 = 0.15 mg/ml) than other fractions (IC 50 = 0.24 mg/ml and 0.21 mg/ml). Accordingly, the lowest molecular weight peptides were further purified and separated using a Sephadex G‐25 column.…”

Anti-Photoaging and Anti-Melanogenesis Effects of Fucoidan Isolated from Hizikia fusiforme and Its Underlying Mechanisms

“…HaCaT cells were seeded in a 24-well plate and incubated for 24 h. The cells were treated with fucoidan (6.25, 12.5, 25, 50, and 100 µg/mL) for 24 h, following which the viability of the cells was determined by MTT assay according to the method described previously [ 49 , 50 ]. The effect of fucoidan on UVB-induced photodamage was then evaluated by measuring the level of intracellular ROS, apoptotic bodies formation, and the viability of UVB-irradiated HaCaT cells by DCF-DA assay, Hoechst 33342 staining, and MTT assay, respectively [ 39 , 48 , 51 , 52 ].…”

Cytoprotective Role of Edible Seahorse (Hippocampus abdominalis)-Derived Peptides in H2O2-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

“…Recently, edible seahorse (H. abdominalis) hydrolysates by enzymatic hydrolysis have been reported to have versatile biological activities, including angiotensin-converting enzyme inhibitory activity, anti-fatigue effect, and cell differentiation [11][12][13]. In addition, this hydrolysate by pepsin and Alcalase ® have also been reported to exert antioxidant activity in oxidative stress-mediated HUVECs and zebrafish models [14,15]. However, bioactive peptides (BAPs) that are responsible for HUVECs protection have not been identified yet, as well as the exact molecular mechanism.…”