Antioxidant activity of polyphenol and ultrasonic extracts from fruits ofCrataegus pentagynasubsp.elburensis

Abstract

The effects of two extracting methods on the total phenolic and total flavonoid contents of Crataegus pentagyna subsp. elburensis Waldst. & Kit. ex Willd fruit extracts were investigated. Antioxidant activities of polyphenol (PP) fraction and ultrasonic (US) extraction were evaluated with four different in vitro antioxidant tests. IC(50) for DPPH radical-scavenging activity was 32.2 ± 1.6 for PP fraction and 36.7 ± 1.5 µg mL(-1) for US extract. Reducing powers of extracts increased with the increase of their concentrations. PP fraction exhibited high reducing power at 2-32 µg mL(-1). Extracts exhibited good H(2)O(2) radical scavenging and Fe(2+) chelating ability. Their high phenolic and flavonoid contents could be responsible for their antioxidant activity and pharmacologic actions.

Antihaemolytic activity of thirty herbal extracts in mouse red blood cells

“…Total phenolic content was determined using the Folin-Ciocalteu method (16). We first mixed sample extract (0.5 mL) with the Folin-Ciocalteu reagent (5 mL, diluted with distilled water at the 1:10 ratio) for 5 min and then added aqueous Na 2 CO 3 (4 mL, 1 mol L -1 ).…”

“…These compounds were extracted from samples according to our recently published paper (16). The extraction was performed twice at 20 °C in a shaking incubator (115 W, Promax 1020, Heidolph, Germany).…”

Antihypoxic activities of Crataegus pentaegyn and Crataegus microphylla fruits-an in vivo assay

“…Hawthorn contains different phenolic and flavonoids compounds, including: chlorogenic acid, epicatechin, rutin, hyperoside and vitexin (Zhang et al, 2001). Previous studies show that hawthorn has high polyphenolic and flavonoids compunds (Rabiei et al, 2012). The aim of the present work was to determine the antihypoxic activities of polyphenol fraction of C. pentaegyna and C. microphylla fruits.…”

“…Total phenolic content was measured calorimetrically using the Folin-Ciocalteau assay (Rabiei et al, 2012). The concentration of total phenolic compounds in the plant extracts was expressed as mg of gallic acid equivalents (GAE) per gram of extract using the equation obtained from standard gallic acid graph: Absorbance = 0.0054 x total phenols [mg gallic acid] + 0.0628(R 2 = 0.987).…”

“…pentaegyna Sub sp. Elburensis (CP) and C. microphylla (CM) fruits were collected from Mazandaran forests in autumn 2012, dried at room temperature, and subjected to polyphenol extraction as descried previously (Rabiei et al, 2012). Extraction was performed two times at 20 °C in a shaking incubator.…”