Antioxidant Activity of Rambutan Honey: The Free Radical-Scavenging Activity in vitro and Lipid Peroxidation Inhibition of Oral Mucosa Wound Tissue in vivo

Abstract

Rambutan honey has been reported to be effective therapeutic in wound healing, may be due to it antioxidant activity, but research of rambutan honey antioxidant activity is still very limited. The purpose of this study is to determine rambutan honey free radicals scavenging activity in vitro and lipid peroxidation inhibition of oral mucosa wound tissue in vivo. The research method was experimental laboratory. The sample used for DPPH test was pure isolated rambutan honey with concentration of 0.5, 1, 2, 4 and 8 mg mLG 1 . Experimental animals used were 64 male Wistar rats. All rats were injured except for the Negative Control group (NC), using the 4 mm-diameter punch biopsy on the palate and then divided into groups of Positive Control (PC), a group with a topical application isolate of Rambutan (RH) 1 mL and group with topical application of Vitamin C (VC) 0.1 mL, then sacrificed at day 0, 3, 7 and 14 and measured lipid peroxidation inhibition of wound tissue with TBARs method. Data were analyzed statistically, using Kruskal Wallis and Mann-Whitney post hoc. The results showed rambutan honey 1 mg mLG 1 have DPPH inhibition of 45.3% and the lipid peroxidation inhibition activity in oral mucosa wound tissue significantly on day 3 (p = 0.028) and 14th (p = 0.037). The conclusions were rambutan honey has antioxidant activity in vitro and in vivo that these natural substances have the potential to be used as an antioxidant drugs in human oral mucosal healing.

Efek Aplikasi Gel Madu Rambutan Pada Mukosa Labial Inferior Terhadap Kadar Malondialdehid (Mda) Saliva

“…Prosedur dimulai dengan memasukkan 400 μl saliva sebelum diaplikasikan gel madu rambutan ke dalam tabung reaksi 1 dan diberi label (Snormal), saliva setelah diaplikasikan gel madu rambutan ke dalam tabung reaksi 2 dan diberi label (Smadu) kemudian kedua tabung ditambah akuades 300μl, larutan SDS 200 μl, larutan BHT 50 μl, larutan EDTA 50 μl, larutan asam asetat 1,5 ml, dan larutan TBA 1,5 ml. Peneliti kemudian menginkubasi semua tabung dalam waterbath suhu 100 °C selama 30 Berdasarkan Tabel 3 dapat diketahui bahwa hasil uji statistika dengan uji Wilcoxon didapatkan nilai sebelum perlakuan p=0,001 dan nilai setelah perlakuan p=0,002. Menurut ahli statistik hasil ini menunjukkan bahwa terdapat perbedaan bermakna nilai kadar MDA saliva sebelum dan setelah diaplikasikan gel madu rambutan secara topikal karena nilai p lebih kecil dari 0,05 (nilai p<0,5).…”

“…Hal ini terbukti dari hasil penelitian diatas yaitu setelah aplikasi gel madu rambutan maka kadar MDA saliva responden menurun. 30…”

Pemberian Madu Rambutan (Nephelium lappaceum) Menurunkan Luas Luka dan Kadar Malondialdehid Tikus Diabetes Melitus

“…Hal ini sesuai dengan penelitian sebelumnya bahwa madu rambutan secara in vitro terbukti menurunkan kadar MDA. 20 Pengaruh madu rambutan dibandingkan dengan kelompok perlakuan lainnya terlihat. Penelitian pada hewan coba dengan proses selular dan humoral kompleks dapat membantu menjelaskan mekanisme aktivitas antioksidan madu rambutan terlihat dari penurunan kadar radikal bebas dalam tubuh tikus diabetes yang mengalami perlukaan.…”

“…Madu rambutan menghambat proses oksidasi dari membran sel dan mencegah kerusakan sel yang berakibat mengganggu penyembuhan luka. 20 Hal ini sangat jelas menunjukkan adanya mekanisme aktivitas antioksidan yang baik dari madu rambutan terhadap peredaman kadar radikal bebas MDA pada penyembuhan luka diabetes sehingga diharapkan apabila bahan alam terstandar ini diaplikasikan akan membantu proses penyembuhan luka diabetes melitus di rongga mulut.…”

The Effect of Rambutan Honey and Rutin on Decrease Blood Glucose and Increase Streptozotocin-Induced Rat Plasma Insulin

“…Flavonoids as antioxidants are able to scavenge free radicals to help regenerate cells, including free radicals that are generated from oxidative stress due to hyperglycemia. Yuslianti reported that rambutan honey contains antioxidant flavonoids of the rutin group of 130.02 μg ml -1 [10,11]. Rakhmat et al reported that the use of rambutan honey at a dose of 0.5 g kg -1 b.wt.…”