Antioxidant Activity of Rubus fraxinifolius Poir. and Rubus rosifolius J. Sm. Leaves

Desmiaty, Yesi; Elya, Berna; Saputri, Fadlina Chany; Hanafi, Muhammad; Prastiwi, Rini

doi:10.5530/jyp.2018.2s.18

Cited by 12 publications

(10 citation statements)

References 9 publications

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“…This method was chosen due to simple. 21 The original color of DPPH was purple. Antioxidant will bleach that purple into yellowish through scavenge free radicals by donating electron.…”

Section: Discussionmentioning

confidence: 99%

Arginase Inhibitory, Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Ethyl Acetate Extract of Caesalpiniaturtuosa Roxb Stem Bark

Atikasari¹,

Hanafi²,

Elya³

2020

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Objective: The purpose of this study is to investigate arginase inhibition, antioxidant activity, total phenolic content and total flavonoid content of ethyl acetate extract of Caesalpiniaturtuosa Roxb. Material and method: stem bark of Caesalpiniaturtuosa Roxb was extracted using hexane, ethyl acetate and methanol subsequently. The ethyl acetate extract was fractioned. Then, the fractions were subjected to arginase inhibition, antioxidant activity, total phenolic content and total flavonoid assay. Correlation was considered by statistical analysis. Result: Out of eight fractions, two fractions have no activity. Two fractions (3 and 6) have strong activity in arginase with inhibition 90.72 % and 91.41% respectively. Fraction 3 and 6 have strong antioxidant activity with IC 50 25.98 µg/mL and 48.01 µg/mL respectively. Statistical analysis shows arginase inhibitor activity was not related with antioxidant activity, total phenolic content and total flavonoid content in this plant. Conclusion: Activity in arginase inhibition of fraction from ethyl acetate extract of Caesalpiniaturtuosa Roxb are not related to antioxidant, total phenolic and flavonoid content.

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“…This method was chosen due to simple. 21 The original color of DPPH was purple. Antioxidant will bleach that purple into yellowish through scavenge free radicals by donating electron.…”

Section: Discussionmentioning

confidence: 99%

Arginase Inhibitory, Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Ethyl Acetate Extract of Caesalpiniaturtuosa Roxb Stem Bark

Atikasari¹,

Hanafi²,

Elya³

2020

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“…As informed in other publications, that R. fraxinifolius fruit and leaves also contain a high polyphenolic compound. 10,21 Phenolic and flavonoid compounds have been reported to present significant antioxidant properties. Phenolic compounds can act as antioxidants because of their ability as reducing agents.…”

Section: Antioxidant Assaymentioning

confidence: 99%

Anti-Elastase, Anti-Tyrosinase and Anti-Oxidant of Rubus Fraxinifolius Stem Methanolic Extract

Desmiaty¹,

Saputri²,

Hanafi³

et al. 2020

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The RFS extract solution and pancreatic elastase (PPE) were mixed in Trizma®-HCl buffer (pH 8.0), then pre-incubated at 25 o C for 5 min. Substrate N-Succinyl-Ala-Ala-Ala-p-nitroanilide (SANA) was added to the mixture and incubated at 25 o C for 20 min in 96-well microplate (Nunc). The optical density due to the formation of p-nitroaniline was ABSTRACT Introduction: Some Rubus were reported had anti-skin aging activity. Rubus fraxinifolius was one of Rubus genus which lives in Indonesian highland. Objective: This study was to examine elastase, tyrosinase, and oxidant inhibitory activity of R. fraxinifolius stem (RFS) extract. Methods: Extraction was done by a Soxhlet apparatus using methanol as solvent. Elastase inhibition activity was determined, which based on the formation of p-nitroaniline. Tyrosinase inhibition activity evaluated based on inhibition of mushroom tyrosinase by the sample with L-DOPA as substrate. The activity of antioxidant was determined using the DPPH radical scavenger method. LC-MS was used for prediction of naturally occurring phytochemicals. Results: The RFS extract yield was 9.03 %. The RFS extract revealed inhibition activity against elastase and tyrosinase with IC 50 128.85 ppm, and 155.19 ppm, respectively. DPPH radical scavenging activity gave IC 50 63.04 ppm. Total phenolic content of the extract was 387.99+3.21 mg GAE/g extract. The LC-MS analysis showed the presence of at least 13 different organic compounds in RFS extract, which might contribute to the bioactivity. Conclusion: Therefore, this experiment further proved that RFS extract might be useful as a natural product ingredient of anti-photoaging skincare products because of its ability to inhibit elastase, tyrosinase, and as an antioxidant.

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“…Rubus rosifolius, known locally as Calingan, is often used as a remedy for stomachaches and mouth ulcers. Rubus rosifolius contains active compounds from phenol derivatives in the form of elagic acid, galik acid, and chlorogenic acid which have antioxidant, antibacterial, anti-thrombotic, and various diseases caused by free radicals [8]. Physalis angulata is known locally as Ciplukan gunung, the fruit is usually consumed to cure thrush, while the boiled water is used for the treatment of heart disease, lung disease, and lowering high blood pressure.…”

Section: Booklet Developmentmentioning

confidence: 99%

Development of Ethnobotany-Based Booklets as Learning Tools for Communities

Lestari¹,

Rohman²,

Utomo³

et al. 2020

Proceedings of the International Conference on Learning Innovation 2019 (ICLI 2019)

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Ethnopharmaceutical research-based booklets are teaching material that contains important information that is presented in a concise and interesting manner for the general public to study. This research aims to develop a booklet for ethnopharmaceutical research-based communities in the Bromo Tengger Semeru National Park area. This research method uses the development adopted from the ADDIE learning model. Data is collected through identification of plants in the field and group discussion forums with the Tengger community. While the booklet's eligibility data was validated by media experts and content material experts, as well as the effectiveness of the booklet being tested on community groups. The results of this study indicate that there are about 40 types of medicinal plants commonly used by the Tengger community. The results of media and content material validation obtained 88.95% and 80% results, which are included in the very valid and quite valid criteria and can be used. The results of the booklet trial on the community group including readability and practicality tests obtained 86.42% and 91.65% which were included in the criteria that were read well and were very practical. Booklet development from the results of research can be a learning media that is more easily understood in learning biology related to plant diversity and utilization.

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Antioxidant Activity of Rubus fraxinifolius Poir. and Rubus rosifolius J. Sm. Leaves

Cited by 12 publications

References 9 publications

Arginase Inhibitory, Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Ethyl Acetate Extract of Caesalpiniaturtuosa Roxb Stem Bark

Arginase Inhibitory, Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Ethyl Acetate Extract of Caesalpiniaturtuosa Roxb Stem Bark

Anti-Elastase, Anti-Tyrosinase and Anti-Oxidant of Rubus Fraxinifolius Stem Methanolic Extract

Development of Ethnobotany-Based Booklets as Learning Tools for Communities

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