Antioxidant activity of several marine skin gelatins

Alemán, Ailén; Giménez, Begoña; Montero, P.; Gómez‐Guillén, M.C.

doi:10.1016/j.lwt.2010.09.003

Cited by 143 publications

(115 citation statements)

References 47 publications

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“…However, a er 9 h of hydrolysis, the highest DH was found in the JCH produced by alcalase (48 %), followed by Protamex (43 %) and trypsin (36 %). This result is in agreement with other studies that reported alcalase to be more efficient than trypsin (26). In our experiment, the parent protein was identical in all three treatments, and hydrolysis conditions were optimized for each enzyme.…”

Section: Degree Of Hydrolysissupporting

confidence: 93%

“…The hydrolysis of all enzymes was fast during the initial stage of the process (3-5 h), but then the rate of hydrolysis gradually decreased until it reached a stationary phase, during which no apparent hydrolysis took place. This result, which shows that most peptides were cleaved within the fi rst 3-5 h of hydrolysis, is in agreement with the classic protease-induced hydrolysis reported for other proteins (26). However, there is a diff erence between the patterns of hydrolysis using Protamex and the other two enzymes.…”

Section: Degree Of Hydrolysissupporting

confidence: 90%

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ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaora sp.)

Barzideh¹,

Latif²,

Gan³

et al. 2014

Food. Technol. Biotechnol.

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SummaryCollagen isolated from the ribbon jellyfi sh (Chrysaora sp.) was hydrolysed using three diff erent proteases (i.e. trypsin, alcalase and Protamex) to obtain bioactive peptides. Angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant activities (i.e. ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity) of the peptides were measured and compared, and the eff ect of the duration of hydrolysis on the bioactivity (ACE inhibitory and antioxidant activities) of peptides was also evaluated. FRAP activity was the highest in Protamex-induced (25-27 mM) and trypsin-induced hydrolysates (24-26 mM) at 7 and 9 h, respectively. Conversely, hydrolysates produced by trypsin for 1 and 3 h showed the highest DPPH radical scavenging activities (94 and 92 %, respectively). Trypsin-induced hydrolysates (at 3 h) also showed the highest ACE inhibitory activity (89 %). The peptide sequences with the highest activities were identifi ed using tandem mass spectrometry, and the results show that the hydrolysates had a high content of hydrophobic amino acids as well as unique amino acid sequences, which likely contribute to their biological activities.

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Section: Degree Of Hydrolysissupporting

confidence: 93%

Section: Degree Of Hydrolysissupporting

confidence: 90%

ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaora sp.)

Barzideh¹,

Latif²,

Gan³

et al. 2014

Food. Technol. Biotechnol.

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“…Among squid species, jumbo squid (Dosidicus gigas), in addition to being the largest known cephalopod, presents a high amount of byproducts produced during its processing (skin, fins, arms, and head) (Lin & Li, 2006), which have collagen as the most prevalent protein (Alemán et al, 2011a;Gildberg et al, 2002). Collagen has a particular molecular structure, which is rich in non-polar amino acids (above 80%) such as glycine, alanine, valine, and proline, and provides collagen specific properties (Kim & Mendis, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Skin collagen has been enzymatically hydrolyzed to recover proteins and peptide fractions. The peptides isolated from squid skin collagen hydrolysates have shown numerous beneficial properties such as antihypertensive, antithrombotic, immunomodulatory, antiproliferative, and antioxidative activities (Alemán et al, 2011a;Gómez-Guillén et al, 2011;Kim & Mendis, 2006). Moreover, it is known that the molecular size, hydrophobicity, and exposition of polar groups of the peptides produced depend on the enzyme used for protein hydrolysis; this also influences their bioactive properties (Kristinsson, 2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro antioxidant, antimutagenic and antiproliferative activities of collagen hydrolysates of jumbo squid (Dosidicus gigas) byproducts

et al. 2015

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Hydrolysates from two different jumbo squid byproducts (fins and arms), produced by trypsin and protease type XIV were compared on the basis of their antioxidant (DPPH and ABTS radical scavenging assays), antimutagenic (Ames test) and antiproliferative (Transformation cell proliferation in M12.C3F6 murine cells) activities. Jumbo squid arms had higher content of collagen than fins, and their hydrolysates had the highest antioxidant activity. Also, jumbo squid arm-derived collagen hydrolyzed with protease XIV showed the highest antimutagenic activity. The four hydrolysates obtained showed low antiproliferative activity, however they are susceptible for further studies to be applied as food additives.

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“…The round scad muscle hydrolysates prepared using alcalase had much higher DH of 60% (Thiansilakul et al, 2007), but in the muscle hydrolysate prepared from yellow stripe trevally, using alcalase, the maximum DH was only 20% after 30 min of hydrolysis (Klompong et al, 2007). In squid, tuna and halibut gelatin hydrolysates prepared using alcalase, the DH recorded were 31, 26 and 19%, respectively after 180 min of hydrolysis (Aleman et al, 2011). Amino acid profile of SPH and their peptide fractions were examined to understand their role in relation to the antioxidative potential.…”

Section: Degree Of Hydrolysismentioning

confidence: 94%

Relationship between antioxidative potential and amino acids composition of the bioactive peptides prepared from Indian squid Uroteuthis (Photololigo) duvaucelii (d’Orbigny, 1835) using alcalase

Fathiraja¹,

Shakila²,

Jeyasekaran³

et al. 2017

Indian J. Fish.

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Biopeptides derived from fish protein hydrolysates are gaining popularity as active ingredients in functional foods. In this study, squid protein hydrolysates (SPH) prepared from Uroteuthis (Photololigo) duvaucelii, using alcalase and their biopeptides fractionated using ultrafiltration were investigated for their antioxidant properties in relation to the changes in their amino acid profiles. Squid mantle hydrolysed within 30 min with a degree of hydrolysis (DH) of 13.7%. SPH had 89% 2, 2-diphenyl-1-picrylhydrazyl (DPPH) inhibition, 94% 2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) inhibition and 96% hydroxyl inhibition at 10 mg ml -1 concentration. In the squid peptides, DPPH inhibition increased with the decrease in the molecular size of peptides from 51% to 95% (3 Ka), ABTS radical inhibition was <42% for peptides with >5KDa size and >95% for peptides with <5KDa. Hydroxyl radical inhibition increased from 75-98% with decrease in molecular size. Increase in glycine, alanine, leucine and taurine was noticed in peptides with MW <5KDa along with a decrease in methionine and arginine. Histidine, asparagine and tyrosine had also contributed to the activities of peptides with MW 3-5 KDa sizes. This study indicated that peptides with MW <5KDa had more free radicals scavenging activities with more hydrophobic amino acids, tyrosine and taurine which are mainly responsible for antioxidative properties. Therefore, squid peptides with MW <5KDa prepared using alcalase have potential to serve as nutraceuticals.

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Antioxidant activity of several marine skin gelatins

Cited by 143 publications

References 47 publications

ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaora sp.)

ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaora sp.)

In vitro antioxidant, antimutagenic and antiproliferative activities of collagen hydrolysates of jumbo squid (Dosidicus gigas) byproducts

Relationship between antioxidative potential and amino acids composition of the bioactive peptides prepared from Indian squid Uroteuthis (Photololigo) duvaucelii (d’Orbigny, 1835) using alcalase

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