Antioxidant Activity of Tartary ( Fagopyrum tataricum (L.) Gaertn.) and Common ( Fagopyrum esculentum Moench) Buckwheat Sprouts

Liu, Chia-Ling; Chen, Yih-Shyuan; Yang, Joan-Hwa; Chiang, Been‐Huang

doi:10.1021/jf072347s

Cited by 136 publications

(86 citation statements)

References 18 publications

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“…The raw neem seed had higher contents of tannic acid, azidiradione, nimbin, nimbidin, salamin and azadirachtin than the defatted neem seed. Quercetin which was slightly retained in the defatted neem seed is well known for its ability to scavenge reactive oxygen species 15 . According to 16 , gedunin is a valuable constituent of neem seed while azadirachtin (abundant in the raw neem seed in 306.23mg/100g) is the most important active constituent.…”

Section: Discussionmentioning

confidence: 99%

Extraction, Isolation and Characterisation of Free and Bound Polyphenols From Neem (Azadirachta Indica Juss) Seed

Awolu¹,

Olufemi²,

Ojo³

et al. 2018

Int. Res. J. Pharm.

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Free and bound polyphenols were extracted from raw and deffated neem seed and then characterized inorder to determine the effect of defatting on the quantity of polyphenols in neem seeds. The total phenolic content, total flavonoid content, total condensed tannin content and DPPH free radical scavenging activities (FRSA) of the raw and defatted neem seed were evaluated. The characterization of the polyphenols was ca rried out using gas chromatography. The chromatogram revealed the presence of a substantial amount of polyphenols in neem seed; the raw neem seed had higher contents of tannic acid, azidiradione, nimbin, nimbdin, salamin andazadirachtin. The DPPH free radical scavenging activities and quercetin content of the defatted neem seed were retained while other bioactive components were reduced.

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Section: Discussionmentioning

confidence: 99%

Extraction, Isolation and Characterisation of Free and Bound Polyphenols From Neem (Azadirachta Indica Juss) Seed

Awolu¹,

Olufemi²,

Ojo³

et al. 2018

Int. Res. J. Pharm.

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“…Extraction of buckwheat sprouts and analysis of phenolic compounds from CBS and TBS were performed as described previously (6). Standard chemicals such as rutin, isoorientin, vitexin, isovitexin and quercetin were applied under the same high-performance liquid chromatography (HPLC) conditions, and the retention times of buckwheat sprout extract components were compared to those of the standards.…”

Section: Analysis Of Phenolic Compounds From Buckwheat Sproutsmentioning

confidence: 99%

Effect of rutin from tartary buckwheat sprout on serum glucose-lowering in animal model of type 2 diabetes

et al. 2016

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This study investigates the anti-diabetic effects of rutin from tartary buckwheat sprout in type 2 diabetes mouse model. The rutin content in tartary buckwheat sprout (TBS) is five times higher than that found in common buckwheat sprout (CBS) as evident from high-performance liquid chromatography analysis. Administration of either rutin or TBS ethanolic extract to diabetes mice decreased the serum glucose level significantly. Rutin down-regulated the expression levels of protein-tyrosine phosphatase 1B; it is negative regulator of insulin pathway, both transcriptionally and translationally in myocyte C2C12 in a dose-dependent manner.In conclusion, rutin can play a critical role in down-regulation of serum glucose level in type 2 diabetes.Keywords: tartary buckwheat sprout, rutin, type 2 diabetes, insulin signaling Buckwheat is a nutritious and pharmaceutically important plant. It contains valuable components that protect human health against inflammation and carcinogenesis (1). Among the main components, rutin, namely rutoside, flavonoid glycoside, is one of the most abundant polyphenols in tartary buckwheat (2). In particular, the amount of rutin has been known to be abundant in buckwheat sprout leaves. Use of buckwheat has been well known in the management of diabetes mellitus. So far, the antidiabetic effect of buckwheat was attributed to D-chiro inositol as a source of insulin mediator in buckwheat seed bran (3). In addition, the seed of tartary buckwheat was reported to improve high glucose-induced insulin resistance and diabetes in mice (4). Protein-tyrosine phosphatase 1B (PTP1B) is known to be a negative regulator of the insulin signaling pathway. Increased expression of PTP1B can regulate the downstream insulin signal transduction, leading to the inhibition of glucose uptake and glycogen synthesis (5). However, there are only few studies about rutin from buckwheat sprouts against diabetes. Thus, we focused on the antidiabetic effects of rutin from tartary buckwheat sprout. The purpose of the present study was to investigate whether the administration of either tartary buckwheat sprout ethanol extract or rutin lowered the serum glucose levels in vivo. In addition, we examined the mechanism of action of rutin with the target gene expression involved in insulin signaling in preadipocyte and myocyte cell lines by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblot analysis. EXPERIMENTAL MaterialsCommon buckwheat sprout (CBS) (Fagopyrum esculentum) and tartary buckwheat sprout (TBS) (Fagopyrum tartaricum) were obtained from the farm of the Chungbuk National University (Cheongju, Republic of Korea). Rutin (purity ≥ 95 %), isoorientin, vitexin, isovitexin and quercetin were purchased from Sigma (USA). Mouse cell lines 3T3-L1 and C2C12 were obtained from the American Type Culture Collection (USA). Male C57BL/6J and diabetes C57BL/6J (B6.Cg-m +/+ Lepr db/J ) mice were kindly provided by the Korea Research Institute of Bioscience and Biotechnology (Republic of Korea). C57BL/6J mouse is a commo...

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“…In connection with such a wide range of healthy activities, efforts are undertaken to apply the most important ingredients of buckwheat in supportive therapy in many diseases. Among these antioxidant components, rutin was recognized as the most health protective, and it has also been proven to be anti-inflammatory and anticarcinogenic [26,27].…”

Section: Introductionmentioning

confidence: 99%

Interaction of skullcap (Scutellaria baicalensis Georgi) and buckwheat (Fagopyrum esculentum Moench) extracts with lipid bilayers

Pruchnik

Włoch

Żyłka

et al. 2015

J Therm Anal Calorim

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In this paper, we report the effect of skullcap (Scutellaria baicalensis Georgi) root (SR) and buckwheat (Fagopyrum esculentum Moench) husk and stalk (BH and BS) extracts on lipid organization and fluidity of model membranes, examined by monitoring of the thermotropic phase transition of dimyristoylphosphatidylcholine (DMPC) with differential scanning calorimetry, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and fluorescence spectroscopy. Despite the very high biological activity of these extracts, such a study has not been undertaken previously. Calorimetric results on the thermotropic parameters of DMPC membranes suggest that BH and BS extracts modify them to a greater extent than SR. Fluorescence and ATR-FTIR results confirm that the compounds contained in the SR extract concentrate in the hydrophilic area of the DMPC bilayer, causing an increase in the order of the polar heads of the lipid, and they do not penetrate the hydrophobic area. Compounds contained in the BS and BH extracts also concentrate in the hydrophilic area of the bilayer; however, they partially affect the hydrophobic-hydrophilic interface of the bilayer as well as the upper part of the acyl chain region.

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Antioxidant Activity of Tartary ( Fagopyrum tataricum (L.) Gaertn.) and Common ( Fagopyrum esculentum Moench) Buckwheat Sprouts

Cited by 136 publications

References 18 publications

Extraction, Isolation and Characterisation of Free and Bound Polyphenols From Neem (Azadirachta Indica Juss) Seed

Extraction, Isolation and Characterisation of Free and Bound Polyphenols From Neem (Azadirachta Indica Juss) Seed

Effect of rutin from tartary buckwheat sprout on serum glucose-lowering in animal model of type 2 diabetes

Interaction of skullcap (Scutellaria baicalensis Georgi) and buckwheat (Fagopyrum esculentum Moench) extracts with lipid bilayers

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