Antioxidant activity of the fractions from water lettuce (Pistia stratiotes) extract

Abstract

Free radicals including reactive oxygen species are continuously increasing in the human body. This condition causes the unbalance between free radicals and antioxidants in the human body. An antioxidant is a compound with the ability to reduce the harmfulness of the free radical. This study aimed to determine the antioxidant activity of fractions and analyzed the functional groups of water lettuce (Pistia stratiotes) methanol extract. The separation process was performed by using thin-layer chromatography (TLC) and column chromatography. The separated fractions were measured for their antioxidant activity by using the 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The functional groups of each fraction were determined by using Fourier-transform infrared (FT-IR) spectroscopy. The separation of water lettuce extract by using column chromatography produced seven fractions with different colors and confirmed by using TLC. The antioxidant activity showed the highest activity in the third fraction with a half-maximal inhibitory concentration (IC50) value of 131.66 ppm. The fifth fraction with the IC50 was about 184.62 ppm. Whereas, the first, second, fourth, sixth, and seventh fractions were relatively weak with the IC50 more than 200 ppm. The FT-IR spectrum also showed that the intensity hydroxyl group in the third fraction higher than the seventh fraction.

In vitro Inhibitory HMG-CoA Reductase Activity of Purified Polyphenol Compounds from Water Lettuce (Pistia Stratiotes) Leaf Extract

“…The polyphenol compound was extracted by the maceration method using 70% ethanol as a solvent at room temperature for 3 hours. 7 Briefly, 20 mg of sample and 200 mL of 70% ethanol were mixed in the Erlenmeyer flask, then stirred using a magnetic stirrer. After 3 hours, the filtrate and residue were separated with a filter paper (Whatman No.…”

“…Water lettuce (Pistia stratiotes) is an aquatic plant that contains some bioactive compounds, such as polyphenols, flavonoids, and tannins. 6,7 A previous study also reported polyphenol compounds show lowering cholesterol activity by inhibiting the HMG-CoA reductase enzyme. It was also reported that flavonoid possess the potential anticholesterol activity and prevent CVDs.…”

INHIBITION OF ALPHA-GLUCOSIDASE BY POLYSACCHARIDES FROM WATER LETTUCE (Pistia stratiotes) LEAF EXTRACTS WITH DIFFERENT EXTRACTION METHODS

“…Water lettuce (Pistia stratiotes) is a floating aquatic plant that is commonly found in Indonesia. A recent study reported that the leaf of this plant contains some bioactive compounds that extracted by aqueous ethanol solvent, such as polyphenols, tannins, and flavonoids (Herpandi et al, 2021). Leaf is also containing other bioactive compounds including polysaccharides.…”

Antioxidant activity of polyphenol compounds extracted from Nypa fruticans Wurmb. (Nipa palm) fruit husk with different ethanol concentration

“…It can act as a radical scavenger by donating hydrogen atoms or electron transfer mechanisms. Polyphenols can be extracted from terrestrial and aquatic plants, such as seaweeds (Ismail et al, 2023), water lettuce (Pistia stratiotes) (Herpandi et al, 2021), and yellow velvetleaf (Limnocharis flava) (Serang & Laili, 2021). A previous study reported that tannin compounds were successfully extracted from coconut (Family Arecaceae) husk using an ethanol solvent (Buamard & Benjakul, 2017).…”