Kalium adalah analit yang penting karena jika ada kekurangan atau terlalu banyak kalium, itu akan menyebabkan kematian seseorang, itulah alasan mengapa kesalahan dalam pengukuran kalium dapat membuat kondisi serius bagi pasien jika terapi berdasarkan pada hasil laboratorium tidak akurat. Keadaan hemokonsentrasi sampel dapat mempengaruhi hasil akhir yang diperoleh. Penelitian ini bertujuan untuk mengetahui teknik aplikasi Tourniquet terhadap kadar kalium, dimana penggunaan Tourniquet yang kurang tepat juga dapat menyebabkan hemokonsentrasi sampel yang dapat meningkatkan kadar kalium dalam serum. Penelitian ini adalah eksperimental laboratorium yang menggunakan serum dari subjek. Semua data kemudian diolah secara statistik dengan T-Test berpasangan. Hasil penelitian adalah rata-rata kadar kalium dengan teknik Tourniquet yang dilepaskan tepat setelah vena dapat diakses adalah sekitar 3,86 mmol / L, lebih rendah dari intervensi kedua yang menggunakan aplikasi Tourniquet yang tidak dilepaskan sampai volume darah cukup, sekitar 4,05 mmol / L. Menurut hasil Uji T berpasangan, nilai p adalah 0,003 dengan signifikansi 2 tailed (α = 0,025) yang membuat p <α. Hasil ini menunjukkan bahwa ada perbedaan kadar potasium antara dua teknik aplikasi turniket

Free radicals including reactive oxygen species are continuously increasing in the human body. This condition causes the unbalance between free radicals and antioxidants in the human body. An antioxidant is a compound with the ability to reduce the harmfulness of the free radical. This study aimed to determine the antioxidant activity of fractions and analyzed the functional groups of water lettuce (Pistia stratiotes) methanol extract. The separation process was performed by using thin-layer chromatography (TLC) and column chromatography. The separated fractions were measured for their antioxidant activity by using the 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The functional groups of each fraction were determined by using Fourier-transform infrared (FT-IR) spectroscopy. The separation of water lettuce extract by using column chromatography produced seven fractions with different colors and confirmed by using TLC. The antioxidant activity showed the highest activity in the third fraction with a half-maximal inhibitory concentration (IC50) value of 131.66 ppm. The fifth fraction with the IC50 was about 184.62 ppm. Whereas, the first, second, fourth, sixth, and seventh fractions were relatively weak with the IC50 more than 200 ppm. The FT-IR spectrum also showed that the intensity hydroxyl group in the third fraction higher than the seventh fraction.

Salted fish is one of the processed fish products with a fairly simple manufacturing process that is only by soaking or applying salt or saline solution and then dried under the sun’s heat until the salt soaks and dries. In making salted fish, there are several ways that can cause the fish to be contaminated with heavy metals such as Lead (Pb). One of them is when drying fish placed on the side of the road, if the fish is consumed by humans can be harmful to human health.The purpose of this study was to determine the effect of pineapple juice on lead levels in sepat salted fish. The research method used in this study is the Experiment Laboratory with post test only control design. This study used an experimental method with a laboratory analysis method with a two-time repetition (duplo) technique. Pineapple juice is an independent variable and lead content in salted fish is the dependent variable. The results showed lead levels in salted fish after being treated with pineapple juice 100%, 75%, 50% and 25% had decreased lead levels in salted fish, this was due to the presence of citric acid content in pineapple levels of lead in salted fish The results showed the effect of pineapple juice on lead levels in sepat salted fish. Decreased levels of lead metals in salted fish sepat caused by a solution of citric acid contained in pineapple fruit extracts.

Hemoglobin is a protein molecule in red blood cells that functions as a means of transporting oxygen (O2) from the lungs to tissues throughout the body and taking carbon dioxide (CO2) from the tissue below to the lungs to be released into the free air. This study aims to determine the effect between blood immediately examined and a delay of 2.5 hours at room temperature on hemoglobin levels conducted at IKesT Muhammadiyah Palembang. The type of research used is cross sectional with intact group comparison research design. The sample consisted of 30 samples immediately examined and 30 samples with a delay of 2.5 hours taken from 30 respondents. The study was conducted starting from patient preparation, taking examination materials, processing examination materials, analysis and research results. The average hemoglobin level immediately examined was 12.8 g/dL while the hemoglobin level with a delay of 2.5 hours was 12.7 g / dL, the results of the wilxocon test showed that the significant value was p=0.342. The p value obtained is p>0.05. The results can be concluded that there is no difference in hemoglobin levels immediately examined and a delay of 2.5 hours at room temperature.

Pemeriksaan laboratorium klinik merupakan suatu sistem yang dapat menentukan keputusan mengenai suatu diagnosis penyakit atau monitoring kesembuhan melalui hasil laboratorium. Tahapan pengendalian mutu laboratorium dibagi menjadi tiga tahapan, yaitu tahap preanalitik, analitik, dan pasca analitik. Kesalahan terbesar pengambilan spesimen darah dalam tahap pra-analitik yaitu terjadinya hemolisis diantara kasus-kasus lain. Serum hemolisis dapat diolah kembali dengan penambahan reagen anti Rh sehingga bisa digunakan dalam pemeriksaan laboratoriu. Sebagai upaya dalam menjawab permasalahan, yang dihadapi petugas laboratorium Puskesmas Mekar Sari mengenai pemanfaatan serum hemolisis, maka perlu dilakukan sosialisasi dan edukasi sehingga dapat meningkatkan pengetahuan dan pemahaman pada petugas laboratorium Mekar Sari. Penjelasan materi mengenai pemanfaatan serum hemolisis dengan penambahan anti Rh melalui media poster, dan mendemonstrasikan akhir dari kegiatan.