Antioxidant activity of the halophyte Limonium tetragonum and its major active components

Abstract

In this study, the antioxidant potentials of crude extracts and solvent-partitioned fractions of Limonium tetragonum were assessed by measuring their ability to scavenge intracellular reactive oxygen species (ROS) generated in HT-1080 cells. Following activity-oriented separation, four flavonol glycosides were isolated as active principles and their chemical structures were determined by 2 D NMR and by comparison with reported spectral data. The isolated compounds (1-4) were evaluated for their antioxidant capacity using three different activity tests; degree of occurrence of intracellular ROS, lipid peroxidation in HT-1080 cells and the extent of oxidative damage of genomic DNA purified from HT-1080 cells. All compounds exhibited significantly inhibited the generation of intracellular ROS and lipid peroxidation in HT-1080 cells, and significantly inhibited DNA oxidation. In addition, direct free radical scavenging effects of these compounds were investigated using the electron spin resonance (ESR) spin-trap technique.

Protective Effects of Ethyl Acetate Soluble Fraction of <i>Limonium tetragonum</i> on Diethylnitrosamine-Induced Liver Fibrosis in Rats

“…9) It has been widely used in treating uterine hemorrhage, oligomenorrhea, dysgalactia, and tinnitus. 10) The edible buds and shoots of L. tetragonum contains several active compounds like myricetin glycosides and quercetin glycosides. 11) Crude extracts and fractions from halophyte L. tetragonum exhibited biological properties such as anticancer and antioxidant activities.…”

“…11) Crude extracts and fractions from halophyte L. tetragonum exhibited biological properties such as anticancer and antioxidant activities. 11,12) Although salt-tolerant plants including L. tetragonum have been recognized as one of the most important ecosystems in a tidal zone, biological and phytochemical studies on halophyte vegetation have been little reported yet.…”

“…10) Besides, the hepatoprotective effect of EtOAc soluble fraction of L. tetragonum extract (EALT) had been detected against liver injury induced by acute alcohol in Sprague-Dawley (SD) rats. 13) The species of Limonium have been used for a long time in the treatment of human hepatitis and the hepatoprotective action of Limonium sinense (GIRARD) KTZE, a traditional Chinese folk medicine, has been well-documented.…”

Antioxidant and Anti-inflammatory Activity of Six Halophytes in Korea

“…[11][12][13][14][15][16][17][18][19][20] Of these, most compounds were reported that were polyphenolic compounds. Polyphenols have been reported to have various biological activities that result in antioxidant, anti-hyperlipidemic, anti-cancer, and anti-inflammatory effects.…”

Anti-obesity Effect of Halophyte Crop, <i>Limonium tetragonum</i> in High-Fat Diet-Induced Obese Mice and 3T3-L1 Adipocytes

“…[16][17][18] Our previous researches mainly focused on the liver protection of L. tetragonum by suppression of hepatic satellite cells (HSC-T6) proliferation, 19) liver injury induced by acute alcohol, 20) and diethylnitrosamine-induced liver fibrosis. 21) However, current studies do not contain any reports on the beneficial effects of certain extract from L. tetragonum in prevention and/or treatment of obesity.…”