Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile

Haddouchi, Farah; Chaouche, Tarik Mohammed; Ksouri, Riadh; Medini, Faten; Sekkal, Fatima Zohra; Benmansour, Abdelhafid

doi:10.1016/s1875-5364(14)60065-0

Cited by 26 publications

(34 citation statements)

References 35 publications

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“…It is more versatile than DPPH because the ABTS model can assess the scavenging activity in both the polar and non‐polar samples, and has the advantage of the working solution being soluble in aqueous and organic solvents over a large range of pH values, with a reaction time that is lower than that of the DPPH assay. These characteristics justify the higher sensitivity found for the antioxidant results of the samples when analysed by the ABTS method, compared with DPPH …”

Section: Resultsmentioning

confidence: 87%

Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity

Seibert

Rodrigues

Carneiro

et al. 2018

Flavour & Fragrance J

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The development of formulations that maintain the biological and physical chemistry properties of essential oils is an important choice when they are used as an active ingredient. This study aimed to characterize the essential oil from leaves of Cymbopogon densiflorus and evaluate the antioxidant activity of the oil, and to produce a nanoemulsion formulation containing it. The essential oil was obtained by hydrodistillation, and seasonality was analysed every 2 months by gas chromatography–mass spectrometry, showing that more than 90% of the composition was maintained for the whole period and that the major compounds were trans‐p‐menta‐2,8‐dien‐1‐ol, cis‐p‐menta‐2,8‐dien‐1‐ol, trans‐p‐menta‐1(7),8‐dien‐2‐ol, cis‐piperitol, and cis‐p‐menta‐1(7),8‐dien‐2‐ol. Stable nanoemulsions were prepared by phase inversion method encapsulating the essential oil. The antioxidant activity was evaluated by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid (ABTS) methods. In the first test, free and nanoemulsified essential oil showed half‐maximal inhibitory concentration (IC50) values equivalent to 14.689 and 3.692 mg mL−1, respectively. In the second test, these values were 0.567 and 0.43 mg mL−1. The development of nanoemulsion‐based essential oil from leaves of C. densiflorus was viable, and the formulated oil was able to reproduce the antioxidant activity at a concentration four times lower than that of the pure essential oil.

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Section: Resultsmentioning

confidence: 87%

Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity

Seibert

Rodrigues

Carneiro

et al. 2018

Flavour & Fragrance J

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“…The concentration of extract at which 50% inhibition is observed (IC 50 ) is calculated in µg/ml. ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt) radical scavenging assay [22] :…”

Section: Quantitative Estimation Of Total Flavonoid Content (Tfc)mentioning

confidence: 99%

“…ABTS radical scavenging assay, unlike DPPH radical scavenging assay can be used at a different pH level, thus making this method useful in studying the antioxidant activity at varying pH levels. Moreover ABTS model can evaluate the scavenging activity of both polar and non-polar samples [22] . The reaction time of ABTS is lower than that of DPPH.…”

Section: Minimum Inhibitory Concentration (Mic) and Minimum Bactericimentioning

confidence: 99%

Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata

Moonmun¹

2017

ijps

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Moonmun, et al.: Antioxidant and Antibacterial Activity of Heliconia rostrataRhizomes of Heliconia rostrate, (family: Heliconiaceae) have been well known for antiophidic property. Ethnobotanically, rhizome of this plant has also been used to cure jaundice, intestinal pain and hypertension. This study aimed at evaluating the antioxidant and antimicrobial potency of two extracts of rhizomes of H. rostrata to find out the one, which exhibited better activity. Methanol and ethanol extract of the dried and defatted rhizomes were prepared and were subjected to qualitative and quantitative estimation of phytoconstituents, antioxidant assay and antibacterial evaluation. The methanol extract was found to give better yield than the ethanol extract. All phytoconstituents detected in the methanol extract were found in the ethanol extract also except for the glycoside content, which was found only in the methanol extract. Total phenolic and flavonoid content was higher in the ethanol extract compared to the methanol extract, whereas total tannin content was higher in the methanol extract. Both ethanol and methanol extracts exhibited antioxidant and antibacterial potency. The antioxidant activities of both extracts were found to be equal, while the ethanol extract exerted significantly higher antibacterial activity compared to the methanol extract. Key words: Heliconiaceae, rhizome, phytoconstituents, antioxidant, antimicrobialMedicinal plants offer alternative therapies with spectacular opportunities [1] . Heliconia rostrata, popularly known as false bird of paradise and hanging lobster claw (family: Heliconiaceae) is a shrub that is reported to contain calcium, nitrogen, potassium, magnesium, iron, phosphorous, chlorine, sulphur, sugar, carbohydrate, starch and protein [2] . This plant is reported to be well known for antiophidic property [3] . Ethnobotanically, the traditional people of Malaysia used these rhizomes to cure jaundice, intestinal pain and hypertension [4] . However, not much of experimental evidence was documented.Oxygen, the most important component for the survival of living being is highly reactive and can do serious damage to healthy cells of the body as a free radical. Oxidation produces free radicals, which can start chain reactions in the cell leading to cell damage and even cell death [5] . An antioxidant terminates these radical reactions by removing free radical intermediates, which causes ageing and disease like atherosclerosis, cancer, cardiovascular diseases and CNS disorders [6] thus, play a vital role in inhibiting or delaying the oxidation at the cellular level [7] . Synthetic antioxidants, butylatedhydoxytoluene (BHT), butylatedhydroxyanisole (BHA) and tert-butyl hydroquinone (TBHQ) are being evaluated in many countries for their potential health hazard and have been found to promote liver damage and causing cancer to laboratory animals [8] . In comparison, the plant sources prove to have less or no side effect [6] , thus channelizing the interest towards the nature for antioxidant potency.Re...

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“…Folk medicinal use of these plants in various diseases was reported by some population in north Africa 1 . Phagnalon saxatile was reported for its use as carminative, analgesic, hypocholestermic 2 and for having potential antioxidant activity 3 . Phytochemical investigations on Phagnalon species are mainly devoted to the study of Phagnalon rupestre species, where it was reported having terpenoids, flavonoids, hydroquinone glycosides, and caffeoylquinic acid derivatives 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Phagnalon saxatile was reported for its use as carminative, analgesic, hypocholestermic 2 and for having potential antioxidant activity 3 . Phytochemical investigations on Phagnalon species are mainly devoted to the study of Phagnalon rupestre species, where it was reported having terpenoids, flavonoids, hydroquinone glycosides, and caffeoylquinic acid derivatives 3 . Phagnalon barbeyanum, which is known locally as Sanuf or Taam Elgamal is an herb growing in Egypt and widely used A benzofuran derivative, 2-[1'carbomethoxyvinyl-(2')]-3-acetoxy-5-[(1'' angeloyloxy ethyl)] 2,3-dihydrobenzofuran, alongside with friedelinol and damaradienyl acetate have been previously isolated from the root of these species, in addition to apigenin and luteolin were isolated from the aerial parts of the same plant 1 .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Investigation and Assessment of Antioxidant and Antimicrobial Activities of Phagnalon barbeyanum Aerial Parts

Kamel

Nafady

Hassanein

et al. 2019

Journal of Advanced Pharmacy Research

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Objectives:The present study aimed to investigate and isolate different phytochemical constituents and assess the antioxidant and antimicrobial activities of the total methanol extract and different fractions of Phagnalon barbeyanum aerial parts. Methods: Phytochemical screening of Phagnalon barbeyanum aerial parts was performed using specific test for each class of compounds. Different chromatographic techniques were used to isolate and purify compounds and their structures were elucidated by using different spectral techniques ( 1 H NMR and 13 C NMR). The antioxidant activity was evaluated by DPPH radical scavenging assay and antimicrobial activity was done by standard agar well diffusion assay. Results: Phytochemical investigation of Phagnalon barbeyanum aerial parts was done for the presence of carbohydrates and or glycosides, sterols and or triterpene, flavonoids, tannins and saponins and revealed the absence of alkaloids and anthraquinones. Compounds; β-sitosterol (1), apigenin (2) and β-sitosterol-3-O-β-Dglucopyranoside (3) were isolated from methylene chloride and ethyl acetate fractions. The ethyl acetate fraction showed highest antioxidant activity followed by n-butanol and methylene chloride fractions. The total methanol extract and n-hexane fractions showed lowest antioxidant activity. The ethyl acetate fraction and total methanol extract showed moderate activity against Staphylococcus aureus and Vancomycin resistant Staphylococcus aureus. Conclusion: The aerial parts of Phagnalon barbeyanum is a rich source of different classes of active constituents as phenolics. The total methanol extract of Phagnalon barbeyanum aerial parts and some of its fractionated concentrates could be considered as antioxidant and antimicrobial agents.

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Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile

Cited by 26 publications

References 35 publications

Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity

Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity

Quantitative Phytochemical Estimation and Evaluation of Antioxidant and Antibacterial Activity of Methanol and Ethanol Extracts of Heliconia rostrata

Phytochemical Investigation and Assessment of Antioxidant and Antimicrobial Activities of Phagnalon barbeyanum Aerial Parts

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