Antioxidant and anti-cholinesterase activity ofSargassum wightii

Abstract

Context: Sargassum has been used in traditional Chinese medicine since the eighth century AD to treat goiter. Sargassum wightii Greville (Sargassaceae) is a major source of alginic acid used widely in food and drug industries. Objective: To evaluate the anti-Alzheimer potential of S. wightii through evaluation of antioxidant and cholinesterase inhibitory activities. Materials and methods: Successive extraction was done using solvents of varying polarity. Solvent extracts (100-500 mg/mL) were employed for all the antioxidant assays. Free radical scavenging activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl, OH. , H 2 O 2 radical scavenging assay. The reducing power of the seaweed was evaluated by ferric reducing antioxidant power and reducing power assay. Cholinesterase (ChE) inhibitory activity was evaluated and the Km, Vmax and Ki were calculated. Further, compound characterization was done by GC-MS analysis. Results: The non-polar extracts were found to possess significant antioxidant activity. At 100 mg/mL, petroleum ether, hexane, benzene and dichloromethane extracts showed significant ChE inhibitory activity with IC 50 values of 19.33 AE 0.56, 46.81 AE 1.62, 27.24 AE 0.90, 50.56 AE 0.90 mg/mL, respectively, for AChE, and 17.91 AE 0.65, 32.75 AE 1.00, 12.98 AE 0.31, 36.16 AE 0.64 mg/mL, respectively, for BuChE. GC-MS reveals that 1,2-benzenedicarboxylic acid, diisooctyl ester is the major compound present in dichloromethane extract of S. wightii. The mode of inhibition exhibited by dichloromethane extract against the cholinesterases was found to be competitive type. Discussion and conclusion: The presence of high amount of terpenoids could be the possible reason for its potential antioxidant and ChE inhibitory activity.

Exploring bioactive fraction of Sargassum wightii: In vitro elucidation of angiotensin-I-converting enzyme inhibition and antioxidant potential

“…SWE was less explored for its medicinal properties; only few studies have explained the biological activity of the crude extract prepared from S. wightii. [14][15][16] In the present study, S. wightii was fractionated using various solvents in Soxhlet extractor. Further, the solvent fraction was subjected to silica gel chromatography to partially purify and identify the solvent fraction rich in bioactive compounds with both ACE inhibition and antioxidant activity.…”

Senyawa-Senyawa Bioaktif Pada Rumput Laut Cokelat Sargassum Sp. : Ulasan Ilmiah

“…1,2-benzenedicarboxylic acid, diisooctyl ester juga dapat menghambat Acetylcholinesterase (AchE) dan Butyrylcholinesterase (BuChE). Oleh karena itu, 1,2-benzenedicarboxylic acid, diisooctyl ester dapat digunakan sebagai salah satu terapi penyakit Alzheimer yang potensial (Syad et al, 2013).…”

Secondary Metabolites and Biological Activity of Invasive Macroalgae of Southern Europe

“…include antifouling activity of S. muticum [110]; anticoagulant [111], antioxidant [112], and anti-inflammatory [113] activity of S. horneri ; antioxidant activity of S. siliquastrum [114,115], S. polycystum [116], and Sargassum sp. [117]; antioxidant and anti-cholinesterase activity of S. wightii [118]; inhibitory effect on lipid peroxidation of S. micracanthum [119]; antimicrobial activity of S. siliquastrum [120]; antipyretic, analgesic, and anti-inflammatory S. fulvellum and S. thunbergii [121]; anti-inflammatory activity of S. Serratifolium [122]; antiallergenic activity of S. tennerimum [123]; anti-diabetic and hypolipidemic activity of S. yezoense [124]; larvicidal activity against malaria vector Anopheles stephensi of S. swartzii [125]; antigenotoxic activity of S. dentifolium [126]; antitumour activity of S. wightii against Dalton’s ascites lymphoma [127] and of S. tenerrimum against Ehrlich ascites carcinoma [128]; and antimelanogenesis activity of S. polycystum [129]. The action of S. fulvellum on skin dermatitis [130] and on neuronal maturation and synaptogenesis [131] is also documented, as well as the chemical genetic effects of S. wightii during embryonic development in zebrafish [132].…”