Antioxidant and Anti-inflammatory Activities of Bauhinia ungulata L. (Fabaceae) on LPS-Stimulated RAW 264.7 Cells

Rodrigues, Rodrigo Rodrigues; Yaochite, Juliana Navarro Ueda; Braga, Milena Aguiar; Sousa, Amanda Ribeiro de; Sasahara, Greyce Luri; Fonseca, Said Gonçalves da Cruz; Araújo, Thiago Dias de Vasconcelos; Santiago, Gilvandete Maria Pinheiro; Sousa, Leôncio M. de; Carvalho, Jarbas Lima de; Nascimento, Francisca Bruna Stefany Aires do; Junior, Helio Vitoriano Nobre; Nagão-Dias, Aparecida Tiemi

doi:10.5530/pj.2019.1.7

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…The EOBU showed a total antioxidant activity of 618.79 mg of ascorbic acid equivalent, indicating a positive correlation between the 0.1 mg.mL −1 concentration of EOBU used and the level of antioxidant activity. Other studies with the ethyl acetate extract from the stem of this plant also has demonstrated its efficiency in reducing Mo (VI) to Mo (V), in addition, it had presented antioxidant activity, with 1.70 mg equivalent of the antioxidant activity of butylated hydroxytoluene (BHT) per milligram of B. ungulata extract, therefore it is being showied greater activity than the BHT that had been used as a positive control before [24] …”

Section: Resultsmentioning

confidence: 95%

“…Other studies with the ethyl acetate extract from the stem of this plant also has demonstrated its efficiency in reducing Mo (VI) to Mo (V), in addition, it had presented antioxidant activity, with 1.70 mg equivalent of the antioxidant activity of butylated hydroxytoluene (BHT) per milligram of B. ungulata extract, therefore it is being showied greater activity than the BHT that had been used as a positive control before. [24] Figure 2 presents the EOBU has reducing power in absorbance value and reduction percentage, respectively. It has been watched that the reducing power of the oil raised as its concentration level.…”

Section: Antioxidant Activitymentioning

confidence: 99%

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In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

Nunes de Andrade Medeiros,

Conrado Bezerra,

José da Silva

et al. 2024

Chemistry & Biodiversity

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Bauhinia ungulate is an antioxidant medicinal plant used in Brazil to lower glycemic index and alternative treatment for diabetes. Therefore, this study aimed to investigate the antioxidant effects of the essential oil of Bauhinia ungulata (EOBU) collected in Boa Vista, Roraima, Brazil, located in the Amazon region. Gas chromatography was used to characterize the components, and antioxidant assays such as DPPH, TAC, reducing power, Fe2+ chelation, and total phenols were performed. The major constituents were molecularly anchored with the human catalase (CAT) enzyme, and maltol was used as a positive control. Among the 25 revealed components, the main ones were α‐bisabolol (27.2%), β‐Caryophyllene (12.5%), Epi‐γ‐eudesmol (13.6%), α‐Humulene (4.5%), and Caryophyllene Oxide (4.3%). The EOBU showed a TAC value of 618.79 mg of ascorbic acid equivalent, free radical scavenging capacity (DPPH) around 43% and 65%, Fe2+ chelation capacity of 161 ± 6 and 126.7 ± 39.6, for 0.1 mg.mL‐1 and 0.5 mg.mL‐1, respectively. Also, 96.5% of total phenols. α‐bisabolol (‐5.7 ± 0.4 Kcal.mol‐1) and β‐caryophyllene (‐6.1 ± 0.5 Kcal.mol‐1) showed good interaction with CAT compared to Maltol (‐4.4 ± 0.4 Kcal.mol‐1). This study demonstrated that EOBU functions as a potent antioxidant, capable of scavenging free radicals and reducing oxidative stress damage.

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Section: Resultsmentioning

confidence: 95%

Section: Antioxidant Activitymentioning

confidence: 99%

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

Nunes de Andrade Medeiros,

Conrado Bezerra,

José da Silva

et al. 2024

Chemistry & Biodiversity

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“…Oxidative stress plays a pivotal role in the pathogenesis of inflammatory diseases, such as cancer and cardiovascular disorders [ 80 , 81 ]. Multiple molecular targets linked to acute and chronic inflammation, growth differentiation, proliferation and apoptosis are modulated by ROS [ 82 ].…”

Section: Resultsmentioning

confidence: 99%

Neocinnamomum caudatum Essential Oil Ameliorates Lipopolysaccharide-Induced Inflammation and Oxidative Stress in RAW 264.7 Cells by Inhibiting NF-κB Activation and ROS Production

et al. 2022

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Neocinnamomum caudatum (Lauraceae) plant is used in the traditional system of medicine and is considered a potential source of edible fruits, spices, flavoring agents and biodiesel. The leaves, bark and roots of the species are used by local communities for the treatment of inflammatory responses, such as allergies, sinusitis and urinary tract infections. However, there is no scientific evidence to support the molecular mechanism through which this plant exerts its anti-inflammatory effect. The aim of the current research was to characterize the chemical constituents of bark (NCB) and leaf (NCL) essential oil of N. caudatum and to elucidate its anti-inflammatory action in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Essential oils extracted by hydrodistillation were further subjected to gas chromatography mass spectrometry (GC-MS) analysis. The major constituents in bark essential oil identified as β-pinene (13.11%), α-cadinol (11.18%) and α-pinene (10.99%), whereas leaf essential oil was found to be rich in β-pinene (45.21%), myrcene (9.97%) and α-pinene (9.27%). Treatment with NCB and NCL at a concentration of 25 µg/mL exerted significant anti-inflammatory activity by significantly reducing LPS-triggered nitric oxide (NO) production to 45.86% and 61.64%, respectively, compared to the LPS-treated group. In the LPS-treated group, the production of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β, decreased after treatment with essential oil, alleviating the mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. The essential oil also inhibited the production of intracellular ROS and attenuated the depletion of mitochondrial membrane potential in a concentration-dependent manner. Pretreatment with NCB also reduced nuclear factor kappa-B (NF-κB)/p65 translocation and elevated the levels of endogenous antioxidant enzymes in LPS-induced macrophages. The present findings, for the first time, demonstrate the anti-inflammatory potential of both bark and leaf essential oils of N. caudatum. The bark essential oil exhibited a significantly more important anti-inflammatory effect than the leaf essential oil and could be used as a potential therapeutic agent for the treatment of inflammatory diseases.

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“…Oxidative stress is reported to be associated with the pathogenesis of several metabolic and chronic disorders, including inflammatory conditions like cancer and cardiovascular disorders [101,102]. There is an increasing amount of evidence that ROS play a crucial role in the occurrence, progression, and resolution of the inflammatory response [103].…”

Section: Msleo Treatment Decreased Lps-induced Increase In Ros Levelsmentioning

confidence: 99%

Mesosphaerum suaveolens Essential Oil Attenuates Inflammatory Response and Oxidative Stress in LPS-Stimulated RAW 264.7 Macrophages by Regulating NF-κB Signaling Pathway

et al. 2023

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Mesosphaerum suaveolens (L.) Kuntze (Syn. Hyptis suaveolens (L.) Poit.) is a wild essential-oil-bearing plant having multiple uses in traditional medicine, perfumery, food, agriculture, and pharmaceutical industries. The present paper is the first report on the in vitro anti-inflammatory effects of the leaf essential oil of M. suaveolens (MSLEO) and unravels its molecular mechanism in LPS-stimulated RAW 264.7 macrophage cells. GC-MS analysis of the essential oil (EO) isolated from the leaves by hydro-distillation led to the identification of 49 constituents, accounting for 90.55% of the total oil, and β-caryophyllene (16.17%), phyllocladene (11.85%), abietatriene (11.46%), and spathulenol (7.89%) were found to be the major components. MSLEO treatment had no effect on the viability of RAW 264.7 cells up to a concentration of 100 μg/mL, and the EO was responsible for a reduction in proinflammatory cytokines like IL-6, IL-1β, and TNF-α, a decrease in intracellular ROS production, and the restoration of oxidative damage by elevating the levels of endogenous antioxidative enzymes like CAT, SOD, GPx, and GSH. RT-qPCR analysis indicated that MSLEO reduced the mRNA expression levels of iNOS and COX-2 as compared to the LPS-induced group. In addition, a confocal microscopy analysis showed that MSLEO inhibited the translocation of NF-κB from the cytosol to the nucleus. The results of this experiment demonstrate that MSLEO possesses significant anti-inflammatory potential by preventing the activation of NF-κB, which, in turn, inhibits the downstream expression of other inflammatory mediators associated with the activation of the NF-κB pathway in LPS-induced RAW 264.7 cells. Thus, the leaf essential oil of M. suaveolens may prove to be a promising therapeutic agent for the treatment of inflammation, and targeting the NF-κB signaling pathway may be considered as an attractive approach for anti-inflammatory therapies.

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Antioxidant and Anti-inflammatory Activities of Bauhinia ungulata L. (Fabaceae) on LPS-Stimulated RAW 264.7 Cells

Cited by 7 publications

References 20 publications

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

In Vitro and In Silico Antioxidant Activity and Molecular Characterization of Bauhinia ungulata L. Essential Oil

Neocinnamomum caudatum Essential Oil Ameliorates Lipopolysaccharide-Induced Inflammation and Oxidative Stress in RAW 264.7 Cells by Inhibiting NF-κB Activation and ROS Production

Mesosphaerum suaveolens Essential Oil Attenuates Inflammatory Response and Oxidative Stress in LPS-Stimulated RAW 264.7 Macrophages by Regulating NF-κB Signaling Pathway

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