Antioxidant and anti-inflammatory effect of polysaccharides from Lobophora variegata on zymosan-induced arthritis in rats

Abstract

This study analyzes the action of sulfated polysaccharides, fucans, from algae Lobophora variegata on zymosan-induced arthritis in rats. Groups of fucans, obtained after acetone fractionation (0.3-2.0 volumes), were denominated F0.3, F0.5, F0.8, F1, F1.5, and F2. The results that F1 contained a high yield in relation to other fractionated fucans. Chemical and structure analysis of F1 was performed by nuclear magnetic resonance (NMR) and infrared (IR) spectroscopies. The in vitro antioxidant activities of the fraction F1 were also observed. Thus, 2 mg/mL of F1 inhibited the phosphomolybdate in the total antioxidant activity assay. The EC(50) values were 0.3 mg/mL and 0.12 mg/mL for superoxide and hydroxyl radicals, respectively. Fucan F1 (25, 50, and 75 mg/kg by body weight), diclofenac sodium (10 mg/kg), and L-NAME (25 mg/kg) were administered intraperitoneally (i.p.) in rats, according to body weight of different groups of animals (n=6). After 6 h, analyses of cell influx and nitrite levels were conducted. Then after 96 h, analysis of edema and concentration of serum TNF-α was carried out along with histopathological analysis. F1 at 25, 50, and 75 mg/kg i.p. by body weight reduced cell influx in 52.1-96.7% and nitric oxide level in 27.2-39% compared with the control group. The reduction of edema and serum TNF-α was observed at 50 mg/kg i.p. (p<0.001). These results suggest that this heterofucan from the brown algae L. variegata has potential anti-inflammatory activity in acute zymosan-induced arthritis in rats and that antioxidant activity promotes modulation in the cellular redox state.

Addition of seaweed (Laminaria digitata) extracts containing laminarin and fucoidan to porcine diets: Influence on the quality and shelf-life of fresh pork

“…Many in vitro antioxidant assays (DPPH, FRAP, TEAC, NO, and ABTS radical scavenging) have demonstrated antioxidant potential of numerous seaweed polysaccharides (Ngo, Wijesekara, Vo, Van Ta, & Kim, 2011) including sulphated polysaccharides and β-glucans from a variety of seaweeds (Turbinaria conoides, Laminaria japonica, Sargassum fulvellum, and Dictyota mertensii) (Costa et al, 2010;Ngo et al, 2011;Paiva et al, 2011;Wang et al, 2010). Limited research exists linking the antioxidant activity of seaweed extracts in vitro with in vivo studies in human or animal subjects (in particular pigs).…”

In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica

“…However, the ratio of fucose in WPS and WPS-3 were 25.74% and 32.51%, respectively. It has been revealed that fucose or fucan were received as critical factor in algal polysaccharides bioactivities [26,27]. Therefore, fucose contents in Laminaria japonica polysaccharides might provide some contributions to their bioactivities.…”

Sulfated fucans extracted from algae Padina gymnospora have anti-inflammatory effect

“…The experimental inflammation models utilized revealed that fucans, like heparin, inhibit the leukocyte migration to the inflammation site (55-70%). Earlier studies show that the fucoidan, or fucan, extracted from the brown alga Fucus vesiculosus, inhibits in a dose-dependent manner, but not specifically, the rolling and migration (Yang et al, 1996;Paiva et al, 2011) of leukocytes in processes mediated by molecules of the selectin family. Furthermore, this polymer is also capable of decreasing injury in models of inflammation caused by non-infectious agents (Ostergaard et al, 2000).…”

“…It is a potent inhibitor of leukocyte migration during the infl ammatory response, owing to its interaction with P and L-selectin (Zang et al, 2001;Klintman et al, 2002). Recently, it was revealed that sulfated fucans from the Fucales and Laminariales orders inhibit leukocyte recruitment in an infl ammation model in rats (Cardoso et al, 2010;Paiva et al, 2011). However, there are no reports of leukocyte migration inhibition by fucans from the Dictyotales order.…”