Antioxidant and anti-inflammatory effects of extracts from Maqui berry Aristotelia chilensis in human colon cancer cells

Abstract

BACKGROUND: Anthocyanins, flavonoids and organic acids widely occurring in extracts of the fruits of Aristotelia chilensis ("Maqui"), concertedly acts on the expression of cyclooxygenase-2 (COX-2), NF-B, HT-29 and Caco-2 colon cancer cell growth inhibition and on the production of inflammatory mediators. OBJECTIVE: To assess the anti-inflammatory effects of extracts from fruits of "Maqui Berry", on the HT-29 and Caco-2 human colorectal cancer cell lines by measuring COX-2 and NF-B as well as their antioxidant activities. MATERIAL AND METHODS: Methanol/water extracts and its partitions (acetone and ethyl acetate) from three varieties of "Maqui" were used to access their effects on growth of HT-29 and Caco-2 colon cancer cells, COX-2, NF-B, NO formation, oxidations by DPPH, TBARS, FRAP and ORAC. RESULTS: Fractions rich in anthocyanins, exhibited potent chemoprotective abilities on decreasing growth of HT-29 and Caco-2 colon cancer cells. The results indicated that the extracts suppressed the production of nitric oxide (NO), through the down-regulation of inducible nitric oxide synthases (iNOS). The structural features of the extracts correlate with the inhibition of NF-B and COX-2 protein expressions and a potent antioxidant activity by assays of DPPH, FRAP, TBARS and ORAC. The inhibition of cell growth and NO production by selected extracts was dose-dependent with significant effects seen at concentration as low as 25.0 and 10.0 ppm, respectively.

Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines

“…The DM‐F2 fraction was selected for further experiments since the American National Cancer Institute considers an extract suitable for further analysis if its LC 50 value is lower than 30 µg/mL (Momtazi‐Borojeni, Behbahani, & Sadeghi‐Aliabadi, 2013). The obtained value (27.6 ± 1.8%) is similar to LC 50 values reported for HT‐29 and Caco‐2 human colon cancer cells (28.50 to 31.64%) when exposed to methanol:water and acetone extracts obtained from Aristotelia chilensis (Céspedes‐Acuña et al., 2018). Moreover, LC 50 were lower than those reported for HT‐29 human colon cancer cells after treatment with phenolic‐rich extracts of several berries such as black raspberry, cranberry, and strawberries, among others (Seeram et al., 2006).…”

Nitric Oxide (NO) and NO Synthases (NOS)-Based Targeted Therapy for Colon Cancer

“…This study provided evidence that MB extracts potently protect against CRC. Moreover, the extracts showed antioxidant and anti-inflammatory abilities in human CRC cells, as the structural feature of extracts might be associated with the inhibition of NF-κB and COX-2 [67].…”

Hydroethanolic Extracts of the Aristotelia Chilensis (Maqui) Berry Reduces Cellular Viability and Invasiveness in the Endometrial Cancer Cell Line Ishikawa

“… 44 Additionally, it has been shown that delphinidin, cyanidin-3-glucoside, and an anthocyanin enriched maqui extract, reduces the expression of Cox2 in HaCaT cells (human keratinocyte), Raw 264.7 (murine macrophage) and Caco-2 cells (human colorectal adenocarcinoma), which has antiapoptotic effects in endometrial cancer. 14 , 18 , 19 , 45 Additionally, other polyphenols present in maqui fruit, such as quercetin, kaempferol, and myricetin, have also been shown to induce apoptosis in some cancer cell lines. 46 - 48 …”