Antioxidant and Anti-Inflammatory Effects of Chaenomeles sinensis Leaf Extracts on LPS-Stimulated RAW 264.7 Cells

Han, Young-Ki; Kim, Yon-Suk; Natarajan, Sithranga Boopathy; Kim, Won‐Suk; Hwang, Jin-Woo; Jeon, Nam-Joo; Jeong, Jae-Hyun; Moon, Sang-Ho; Jeon, Byong-Tae; Park, Pyo-Jam

doi:10.3390/molecules21040422

Cited by 33 publications

(26 citation statements)

References 33 publications

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“…The role of the metabolites detected in this study has been described in numerous studies [23][24][25]. Earlier studies have reported that phenolic compounds inhibit the production of NO in LPS-stimulated RAW 264.7 cells [26][27][28]. It is most likely that the greater NO inhibitor capacity shown by 1A was mainly influenced by the higher amount of phenolic compounds.…”

Section: Correlation Between Metabolite Profiles and No Inhibitory Acmentioning

confidence: 84%

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Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

et al. 2016

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This study was aimed at examining the variations in the metabolite constituents of the different Ajwa grades and farm origins. It is also targeted at establishing the correlations between the metabolite contents and the grades and further to the nitric oxide (NO) inhibitory activity. Identification of the metabolites was generated using 1 H-NMR spectroscopy metabolomics analyses utilizing multivariate methods. The NO inhibitory activity was determined using a Griess assay. Multivariate data analysis, for both supervised and unsupervised approaches, showed clusters among different grades of Ajwa dates obtained from different farms. The compounds that contribute towards the observed separation between Ajwa samples were suggested to be phenolic compounds, ascorbic acid and phenylalanine. Ajwa dates were shown to have different metabolite compositions and exhibited a wide range of NO inhibitory activity. It is also revealed that Ajwa Grade 1 from the al-Aliah farm exhibited more than 90% NO inhibitory activity compared to the other grades and origins. Phenolic compounds were among the compounds that played a role towards the greater capacity of NO inhibitory activity shown by Ajwa Grade 1 from the al-Aliah farm.

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Section: Correlation Between Metabolite Profiles and No Inhibitory Acmentioning

confidence: 84%

“…that phenolic compounds inhibit the production of NO in LPS-stimulated RAW 264.7 cells [26][27][28]. It is most likely that the greater NO inhibitor capacity shown by 1A was mainly influenced by the higher amount of phenolic compounds.…”

Section: Correlation Between Metabolite Profiles and No Inhibitory Acmentioning

confidence: 99%

Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

et al. 2016

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“…LPS‐induced inflammation can involve various signaling mechanisms in immune cells, such as the MyD88‐dependent pathway (myeloid differentiation factor 88, MyD88) and the TRIF‐dependent pathway (Toll‐interleukin‐1R domain‐containing adapter inducing interferon‐ β , TRIF) (J. Guo & Friedman, ; Han et al, ; Sun et al, ). The MyD88‐mediated pathway plays a crucial role in phosphorylation of mitogen‐activated protein kinase (MAPK), activation of nuclear factor‐kappa B (NF‐ κ B) and activator protein (AP‐1), which leads to the initiation of transcription factors.…”

Section: Resultsmentioning

confidence: 99%

Identification of interactions between multiple components in Socheongryong‐tang using a plant profiling approach

Lee

Kang

et al. 2019

Biomedical Chromatography

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Traditional herbal medicine consists of multiple components. There are interactions among the components, which affect both potency and toxicity. The preparation of herbal medicines can be a cause of interactions between multicomponents in herbs.To demonstrate the differences in multiherb interactions based on the preparation methods, the changes in the active components in the different preparations of Socheongryong-tang (SCRT) were evaluated using metabolomics profiling. We performed multicomponent profiling of the decoction of SCRT (SCRTD) and individual herb mixture (SCRTM) using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Active compounds from SCRTD and SCRTM were identified using multivariate analysis, and the activities between the two groups were compared. We also evaluated the antiinflammatory effect of SCRT through investigating the protein expression of iNOS and COX-2 in lipopolysaccharide-induced macrophage RAW 264.7 cells in both groups. From the multivariate analysis, 53 active compounds that have different intensities between SCRTD and SCRTM were identified. The intensities of those components, such as ephedrines, glycyrrhizic acid, 6-gingerol and (2E,4E,8Z,10E)-Nisobutyl-2,4,8,10-dodecatetraenamide, which is newly identified in Asiasarum heterotropoides, were mostly higher in SCRTD than in SCRTM, which was related to the anti-inflammatory effect. From the iNOS inhibition test, it was found that SCRTD had a stronger anti-inflammatory effect than SCRTM. It was demonstrated that multicomponent interactions can be changed by the preparation method, and finally the anti-inflammatory effect in SCRT can be affected. KEYWORDS multicomponent interactions, plant profiling approach, Socheongryong-tang, UPLC-QTOF-MS Abbreviations: AP-1, activator protein-1; GC, gas chromatography; IRF-3, interferon regulatory factor-3; LC, lipid chromatography; LPS, lipopolysaccharide; MAPK, mitogen-activated protein kinase; MyD88, myeloid differentiation factor 88; NF-κB, activation of nuclear factor-kappa B; PCA, principal component analysis; PLS-DA, partial least square discriminant analysis; QTOF, ultra-high performance liquid chromatography quadrupole time-of-flight; SCRT, Socheongryong-tang; SCRT_PM, mixture of original plants; SCRTD, decoction of Socheongryong-tang; SCRTM, individual herb mixture of Socheongryong-tang; SHXXT, San-Huang-Xie-Xin-Tang; STAT1, single transducers and activators of transcription 1; TRIF, toll-interleukin-1R domain-containing adapter inducing interferon-β

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“…Previous report from other groups shows that the water extracts of Chaenomeles sinensis have higher antioxidant activity whereas the ethanolic extracts have higher anti-inflammatory activity (34). Another report demonstrates that the polar fractions of Suaeda asparagoides have higher antioxidant activity than the non-polar fractions whereas the non-polar fractions have higher anti-inflammatory activity than the polar fractions (35).…”

Section: Discussionmentioning

confidence: 96%

Effect of rice variety and modification on antioxidant and anti-inflammatory activities

Okonogi

Kaewpinta

Junmahasathien

et al. 2018

DD&T

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The effects of variety and modification of rice on its antioxidant and anti-inflammatory activities were investigated. White rice varieties; Jasmine (JM) and Saohai (SH), and pigmented rice varieties; Doisket (DS) and Homnil (HN) were used. The modified rice samples were obtained from chemical modification using etherification reaction. The activities of the modified rice samples were compared with the ethanol extracts of the raw rice at the same rice concentration. Antioxidant activity was measured by the free radical scavenging activity tests and ferric reducing power assay. Results indicated that the ethanol extracts of raw rice had higher antioxidant activity than the modified rice. Among the raw rice tested, the pigmented rice showed higher antioxidant activity than white rice. Trolox equivalent antioxidant capacity values from free radical scavenging activity test were revealed that 50% ethanol extracts of HN and DS possessed the highest antioxidant activity. Ferric reducing power assay showed that 50% ethanol extracts of DS had the highest antioxidant activity. The anti-inflammatory activity was evaluated in vitro using a lipopolysaccharide-stimulated RAW264.7 macrophage cell model with enzyme-linked immunosorbent assay. Absolute ethanol extracts of HN reduced interleukin-6 secretion whereas that of DS suppressed interleukin-6 and tumor necrosis factor -α secretion. These results indicate that variety of rice, chemical modification, and extracting solvent were the factors that play an important role on antioxidant and anti-inflammatory activity. This study supports the potential use of the pigmented rice, especially DS, as a promising choice of a natural source because of its antioxidant and anti-inflammatory activities.

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Antioxidant and Anti-Inflammatory Effects of Chaenomeles sinensis Leaf Extracts on LPS-Stimulated RAW 264.7 Cells

Cited by 33 publications

References 33 publications

Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

Discrimination and Nitric Oxide Inhibitory Activity Correlation of Ajwa Dates from Different Grades and Origin

Identification of interactions between multiple components in Socheongryong‐tang using a plant profiling approach

Effect of rice variety and modification on antioxidant and anti-inflammatory activities

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