Antioxidant and Anti-Inflammatory Effects of Curcumin Nanoparticles on Drug-Induced Acute Myocardial Infarction in Diabetic Rats

Abstract

We have investigated the cardio-protective effects of pretreatment with curcumin nanoparticles (CUN) compared to conventional curcumin (CUS) on the changes in oxidative stress parameters and inflammatory cytokine levels during induced acute myocardial infarction (AMI) in rats with diabetes mellitus (DM). DM was induced with streptozotocin, and AMI with isoproterenol. Eight groups of seven Wister Bratislava rats were included in the study. The N-C was the normal control group, AMI-C was the group with AMI, DM-C was the group with DM, and DM-AMI-C was the group with DM and AMI. All four groups received saline solution orally during the whole experiment. S-DM-CUS-AMI and S-DM-CUN-AMI groups received saline for seven days prior to DM induction and continued with CUS (200 mg/kg bw, bw = body weight) for S-DM-CUS-AMI and CUN for S-DM-CUN-AMI (200 mg/kg bw) for 15 days before AMI induction. The CUS-DM-CUS-AMI group received CUS (200 mg/kg bw), while the CUN-DM-CUN-AMI received CUN (200 mg/kg bw) for seven days prior to DM induction, and both groups continued with administration in the same doses for 15 days before AMI induction. CUS and CUN prevented elevation of creatine kinase, creatine kinase-MB, lactate dehydrogenase in all groups, with better results in the CUN (S-DM-CUN-AMI and CUN-DM-CUN-AMI groups). CUS and CUN significantly reduced serum levels of oxidative stress markers (malondialdehyde, the indirect assessment of nitric oxide synthesis, and total oxidative status) and enhanced antioxidative markers (total antioxidative capacity and thiols, up to 2.5 times). All groups that received CUS or CUN showed significantly lower serum levels of tumor necrosis factor-alpha, interleukin-6, and interleukin-1β. The best antioxidative and anti-inflammatory effects were obtained for the group that received CUN before DM induction (CUN-DM-CUN-AMI group). Pretreatment with CUN proved higher cardio-protective effects exerting an important antioxidative and anti-inflammatory impact in the case of AMI in DM.

Curcumin Prevents Cyclophosphamide-Induced Lung Injury in Rats by Suppressing Oxidative Stress and Apoptosis

“…Therefore, different approaches, including the use of liposomes, adjuvants, and nanoparticles have been applied to increase the solubility of CUR [61]. The antioxidant and anti-inflammatory effects of CUR nanoparticles have been recently demonstrated in acute myocardial infarction in diabetic rats [62]. In comparison with the conventional form, nano CUR exhibited stronger antioxidative and anti-inflammatory effects [62].…”

“…The antioxidant and anti-inflammatory effects of CUR nanoparticles have been recently demonstrated in acute myocardial infarction in diabetic rats [62]. In comparison with the conventional form, nano CUR exhibited stronger antioxidative and anti-inflammatory effects [62]. Hence, the potential of nano CUR to protect against CP-induced oxidative stress, inflammation, and lung injury is worth being investigated.…”

The Effect of Nano-Epigallocatechin-Gallate on Oxidative Stress and Matrix Metalloproteinases in Experimental Diabetes Mellitus

“…route) from retro-orbital sinus, followed by rat euthanasia by cervical dislocation [43]. Rats with glucose higher or equal to 200 mg/dL were considered to have diabetes mellitus [20].…”

“…One of the most used experimental models for testing various therapies addressing type 1 DM is based on STZ administration. STZ induces type 1 DM, with destruction of pancreatic beta cells and associated insulin deficiency, as a result of its cytotoxic effect, mediated by increased synthesis of reactive oxygen species (ROS) and subsequent inflammation [18][19][20]. A protective effect of EGCG on pancreatic beta cells has been already demonstrated in experimental studies [21]; meanwhile, oral chronic administration of EGCG proved to have hypoglycemic and hypolipidemic effects and to reduce oxidative stress in streptozotocin-diabetic rats [22].…”

Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the up-regulation of nuclear factor erythroid 2-related factor 2 and decrease blood pressure in rats

“…Gingerols (6-, 8-, and 10-gingerols) are the major polyphenols present in ginger, and with heat treatment, they are transformed into corresponding shogaols, and upon hydrogenation to paradols (9) . Curcuminoids (curcumin demethoxycurcumin, 5'-methoxycurcumin, and dihydrocurcumin) are colouring agents present in turmeric, with improved health benefits as a nanoparticle, and known to beneficially modulate inflammation under diabetic conditions (10,11) . Since ginger and turmeric are known to exhibit health benefits (11,12) , we hypothesized that lipid-soluble molecules may migrate to the oil from these spices (ginger and turmeric) during the heating process might attenuate the consequences of heated oil consumption.…”