Antioxidant and Antidiabetic Activities of Methanolic extract of Bark of Cinnamomum zeylanicum in Diabetic Rats

Abstract

Background: Cinnamomum zeylanicum or Ceylon cinnamon is used for the management of dyspepsia, dysmenorrhea, memory loss and tremor. It is also traditionally recommended for the management of diabetes. Further studies are required to explore the antioxidant and antidiabetic activity of various extracts of bark of C. zeylanicum. Hence, the present study is planned to investigate the antioxidant and antidiabetic activities of methanolic extract of barks of C. zeylanicum. Methods: Bark of C. zeylanicum was extracted with methanol, ethanol and acetone and its antioxidant activity was studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assays. Acute toxic effect of methanolic extract of C. zeylanicum (MECZ) studied as per the method described in OECD Guideline for testing of chemicals (Test Guideline 423). MECZ was studied for its antidiabetic effect using streptozotocin-induced diabetic rats. Results: In both DPPH and ABTS free radical scavenging assay, methanolic and acetone extracts exhibited free radical scavenging activity, respectively. In acute toxicity testing, MECZ did not show any significant toxic signs up to 2000 mg/kg, hence the antidiabetic activity of MECZ was carried out at the dose levels of 125, 250 and 500 mg/kg. MECZ showed antidiabetic activity from 2 nd week of the experiment onward. In the biochemical analysis, MECZ treated animals showed significant decreases in the levels of ALP and urea when compared with control. In lipid profile analysis, diabetic animals and diabetic animals treated glibenclamide showed significant increases in the levels of total cholesterol when compared with normal control and MECZ prevented the STZ-induced hyperlipidemia. At the end of the study, diabetic animals and diabetic animals treated with glibenclamide and MECZ showed significant decreases in the level of insulin when compared with the control group. In the histopathological analysis, sections from the liver, pancreas and kidney of the diabetic animals showed mid-to-moderate toxic effects and glibenclamide and MECZ 500 mg/kg prevented the STZ-induced cellular changes. Conclusion: The MECZ exhibited significant antioxidant and antidiabetic activities.

Biochemical, structural characterization and in-vitro evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of Cinnamomum zeylanicum bark extract

“…Our findings are agreed with the previous studies (Husain et al, 2018;Singh et al, 2020;Madushika Wariyapperuma et al, 2021). Wickramasinghe et al (2018) reported that methanolic nanosuspensions and extract of cinnamon bark contained a high amount of TPCs (27.64 ± 2.70 mg GAE/g).…”

“…A recent study by Madushika Wariyapperuma et al ( 2021) investigated the cinnamon extracts and silver nanoparticles showed high free radical scavenging activity at a concentration of 0.009 mg/mL. Another study reported by Singh et al (2020) investigated that ethanolic extract and nanosuspensions of C. zeylanicum showed 87.33% ± 0.42% free radical scavenging activity at 1,000 μg/mL concentration. Our findings are consistent with the previous studies.…”

"Phytochemical Screening, Anti-Oxidant and Anti- Hyperglycaemic Activity Determination of PHYLLANTHUS EMBLICA"

“…After receiving STZ, 80% of the mice developed diabetes, and 10% of the animals died from STZ-induced hypoglycemia. Moreover, the normal [43] control rats showed steady BW gain throughout the 21 days of treatment, while the diabetic control rats showed significant BW reduction. However, the treatment with the Phyllanthus emblica fruits ethanolic extract (200 and 400 mg/kg) and glibenclamide for twenty-one days led to a significant increase in BW of diabetic animals.…”

Effect of permeation enhancer on bioavailability of formulated patches of amoxicillin

“…Cinnamon Bark consists of dehydrated bark inside bark of Cinnamomum zeylanicum Nees, belonging to family Lauraceae. 11,12 Cinnamon Bark contains 0.5 to 1.0% of volatile oil, 1.2 % of tannis mucilage, calcium oxalate, starch and a sweet substance is known as mannitol and is used for gastric trouble, loose motion, and has anti flatulence. It is also used for increasing appetite; it acts as antibacterial and antiparasites zeylanicum Nees, having a place with family Lauraceae.11,12 Cinnamon Bark contains 0.5 to 1.0% of unstable oil, 1.2 % of tannis adhesive, calcium oxalate, starch and a sweet substance is known as mannitol and is utilized for gastric difficulty, free movement, and has hostile to tooting.…”