Antioxidant and antidiabetic properties of bioactive peptides from soursop (Annona muricata) leaf biomass

Lee, Chia Hau; Hamdan, Norfadilah; Ling, Luo Ing; Wong, Syieluing; Nyakuma, Bemgba Bevan; Khoo, Shing Ching; Ramachandran, H. D.; Jamaluddin, Haryati; Wong, Keng Yinn; Lee, Ting Hun

doi:10.1007/s13399-023-03993-5

Cited by 3 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination of the free radical activity of ABTS was based on the study of Lee et al [13], with some modifications. The ABTS solution was prepared in a 1: 1 (v/v) ratio of 7 mM ABTS and 2.45 mM potassium persulfate, and a solution of 5 mM PBS was mixed and left in the dark for 12-16 h. Before use, the ABTS free radical solution was kept sealed from light, and the initial absorbance at 734 nm was determined by diluting the stock solution with PBS to give an initial absorbance of 0.70 ± 0.02.…”

Section: Measurement Of Abts Radical Scavenging Capacitymentioning

confidence: 99%

Preparation, Isolation and Antioxidant Function of Peptides from a New Resource of Rumexpatientia L. ×Rumextianshanicus A. Los

Liu,

Wang,

Hong

et al. 2024

Foods

View full text Add to dashboard Cite

Rumexpatientia L. ×Rumextianshanicus A. Los (RRL), known as “protein grass” in China, was recognized as a new food ingredient in 2021. However, the cultivation and product development of RRL are still at an early stage, and no peptide research has been reported. In this study, two novel antioxidant peptides, LKPPF and LPFRP, were purified and identified from RRL and applied to H2O2-induced HepG2 cells to investigate their antioxidant properties. It was shown that 121 peptides were identified by ultrafiltration, gel filtration chromatography, and LC-MS/MS, while computer simulation and molecular docking indicated that LKPPF and LPFRP may have strong antioxidant properties. Both peptides were not cytotoxic to HepG2 cells at low concentrations and promoted cell growth, which effectively reduced the production of intracellular ROS and MDA, and increased cell viability and the enzymatic activities of SOD, GSH-Px, and CAT. Therefore, LKPPF and LPFRP, two peptides, possess strong antioxidant activity, which provides a theoretical basis for their potential as food additives or functional food supplements, but still need to be further investigated through animal models as well as cellular pathways.

show abstract

Section: Measurement Of Abts Radical Scavenging Capacitymentioning

confidence: 99%

Preparation, Isolation and Antioxidant Function of Peptides from a New Resource of Rumexpatientia L. ×Rumextianshanicus A. Los

Liu,

Wang,

Hong

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…RSM can be applied to identify the optimal levels of parameters, such as enzyme type, enzyme:substrate ratio, hydrolysis time, and temperature, that maximize the anti-LOX activity of protein hydrolysates. The RSM approach has been used in previous studies to optimize the yield of bioactive protein hydrolysates and peptides [61,62].…”

Section: Future Directionsmentioning

confidence: 99%

Bioactive Peptides and Protein Hydrolysates as Lipoxygenase Inhibitors

Wong

Chai

2023

Biology

View full text Add to dashboard Cite

Lipoxygenases are non-heme iron-containing enzymes that catalyze the oxidation of polyunsaturated fatty acids, resulting in the production of lipid hydroperoxides, which are precursors of inflammatory lipid mediators. These enzymes are widely distributed in humans, other eukaryotes, and cyanobacteria. Lipoxygenases hold promise as therapeutic targets for several human diseases, including cancer and inflammation-related disorders. Inhibitors of lipoxygenase have potential applications in pharmaceuticals, cosmetics, and food. Bioactive peptides are short amino acid sequences embedded within parent proteins, which can be released by enzymatic hydrolysis, microbial fermentation, and gastrointestinal digestion. A wide variety of bioactivities have been documented for protein hydrolysates and peptides derived from different biological sources. Recent findings indicate that protein hydrolysates and peptides derived from both edible and non-edible bioresources can act as lipoxygenase inhibitors. This review aims to provide an overview of the current knowledge regarding the production of anti-lipoxygenase protein hydrolysates and peptides from millet grains, chia seeds, insects, milk proteins, fish feed, velvet antler blood, fish scales, and feather keratins. The anti-lipoxygenase activities and modes of action of these protein hydrolysates and peptides are discussed. The strengths and shortcomings of previous research in this area are emphasized. Additionally, potential research directions and areas for improvement are suggested to accelerate the discovery of anti-lipoxygenase peptides in the near future.

show abstract

Antihypertensive, Antidiabetic, and Antioxidant Properties of Novel Azolla pinnata Fern Protein Hydrolysates: Inhibition Mechanism, Stability, Profiling, and Molecular Docking

Qoms,

Arulrajah,

Ibadullah

et al. 2024

Food Bioprocess Technol

View full text Add to dashboard Cite

Antioxidant and antidiabetic properties of bioactive peptides from soursop (Annona muricata) leaf biomass

Cited by 3 publications

References 42 publications

Preparation, Isolation and Antioxidant Function of Peptides from a New Resource of Rumexpatientia L. ×Rumextianshanicus A. Los

Preparation, Isolation and Antioxidant Function of Peptides from a New Resource of Rumexpatientia L. ×Rumextianshanicus A. Los

Bioactive Peptides and Protein Hydrolysates as Lipoxygenase Inhibitors

Antihypertensive, Antidiabetic, and Antioxidant Properties of Novel Azolla pinnata Fern Protein Hydrolysates: Inhibition Mechanism, Stability, Profiling, and Molecular Docking

Contact Info

Product

Resources

About