Antioxidant and antimicrobial activity of bioactive prodigiosin produces from Serratia marcescens using agricultural waste as a substrate

Arivizhivendhan, K. V.; Mahesh, M.; Boopathy, R.; Swarnalatha, S.; Mary, R. Regina; Sekaran, G.

doi:10.1007/s13197-018-3188-9

Cited by 68 publications

(53 citation statements)

References 36 publications

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“…activity of the DPPH radical scavenging capacity of PG to be 86%, 99%, and 98%, respectively. However, few data on the ABTS radical scavenging of PG have been reported so far [35]. Notably, almost all of the studies did not report the IC50 values on antioxidant activity of PG [11].…”

Section: Antioxidant and Anticancers Activities Of Purified Pgmentioning

confidence: 99%

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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This study aimed to establish the culture process for the cost-effective production of prodigiosin (PG) from demineralized crab shell powder (de-CSP), a fishery processing byproduct created via fermentation. Among the tested PG-producing strains, Serratia marcescens TNU02 was demonstrated to be the most active strain. Various ratios of protein/de-CSP were used as the sources of C/N for PG biosynthesis. The PG yield was significantly enhanced when the casein/de-CSP ratio was controlled in the range of 3/7 to 4/6. TNU02 produced PG with a high yield (5100 mg/L) in a 15 L bioreactor system containing 4.5 L of a newly-designed liquid medium containing 1.6% C/N source (protein/de-CSP ratio of 3/7), 0.02% (NH4)2SO4, 0.1% K2HPO4, and an initial pH of 6.15, at 27 °C for 8 h in dark conditions. The red pigment was purified from the culture broth and then quantified as being PG by specific Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and UV spectra analysis. The purified PG demonstrated moderate antioxidant and effective inhibition against four cancerous cell lines. Notably, this study was the first to report on using crab wastes for PG bioproduction with high-level productivity (5100 mg/L) in a large scale (4.5 L per pilot) in a short period of fermentation time (8 h). The salt compositions, including (NH4)2SO4 and K2HPO4, were also a novel finding for the enhancement of PG yield by S. marcescens in this report.

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Section: Antioxidant and Anticancers Activities Of Purified Pgmentioning

confidence: 99%

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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“…Prodigiosin has been investigated for its vast biological activities, including insecticidal, antioxidant, algicidal, antimicrobial, anti-inflammatory, antimalarial, anticancer, immunomodulatory, and anti-diabetic activities [19,37]. With the aim of evaluating the prodigiosin newly synthesized in this study for potential use in anticancer drugs, prodigiosin was tested for its inhibition against some cancerous cell lines, including A549, Hep G2, MCF-7, and WiDr.…”

Section: Evaluation Of Inhibitory Effect Of Prodigiosin Against Cancementioning

confidence: 99%

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

et al. 2019

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Marine chitins (MC) have been utilized for the production of vast array of bioactive products, including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds. The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG), via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011 with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of 5.65–6.15 containing 1% α-chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was kept at 25 °C for 2 d. Notably, α-chitin was newly investigated as the major potential material for PG production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06, 0.04, 0.04, and 0.2 µg/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold efficacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest that marine chitins are valuable sources for production of prodigiosin, a potential candidate for cancer drugs.

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“…PSN0.75 had the highest DPPH value comparing with other nectar samples recording 88.17% at 120 min of incubation time, while, prodigiosin recorded 66.87 % and ascorbic acid 93.27%. Arivizhivendhan et al, (2018) reported that the bioactive prodigiosin extracted from Serratia effectively scavenged the free radical and inhibited the bacterial growth in food products. Some amino acids, such as histidine, tyrosine, methionine, and cysteine, have been reported to show antioxidant activity (Abdel-Hamid et al, 2017;Osman et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The prodigiosin having pyrrolyl pyrromethane skeleton was first charactarized from S. marcescens and reported to be antimicrobial including antibacterial activity (Gulani et al, 2012), antifungal, antiproliferative agent and immunosuppressive (Khanafari et al, 2006). Prodigiosin has prohibited the growth of food borne pathogens effectively and the shelf life of the food was also extended significantly (Arivizhivendhan et al, 2018). The prodigiosin of Serratia marcescens IBRL USM 84 exhibited a broad inhibitory spectrum activity against more bacteria (Nwankwo et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Sensory Evaluation, Antioxidant, Antimicrobial Activities and Colour of Strawberry Nectar Enriched With Prodigiosin Pigment Produced From Serratia Marcescens

Salama¹,

Toliba²,

Akl³

2019

Pak J. Biotech

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This study was performed to evaluate the impact of natural red pigment (prodigiosin) extracted from local microorganism of Serratia marcescens as a natural colouring, antioxidant and antimicrobial agents in strawberry nectar. The local S. marcescens was identified by morphological, biochemical tests as well as sequencing a fragment of the 16S rDNA gene as new Egyptian strain namely S. marcescens A1A. The antioxidant scavenging of prodigiosin was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antibacterial activity of prodigiosin was assayed against three bacterial indicators (B. cereus, Staph. aureus and E. coli) by measuring the inhibition zones using discdiffusion assay on Muller Hinton Agar. The results revealed that the free radical scavenging activity was increased in the prepared solutions with increasing the concentration of prodigiosin. The prodigiosin of S. marcescens A1A has prohibited the growth of tested bacteria effectively. On the other hand, the fortification of strawberry nectar with prodigiosin pigment improved the antimicrobial, antioxidant activities, colour, taste, clarity, thickness and overall acceptability of resulted products.

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Antioxidant and antimicrobial activity of bioactive prodigiosin produces from Serratia marcescens using agricultural waste as a substrate

Cited by 68 publications

References 36 publications

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

Sensory Evaluation, Antioxidant, Antimicrobial Activities and Colour of Strawberry Nectar Enriched With Prodigiosin Pigment Produced From Serratia Marcescens

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