ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Tinospora crispa (putarwali) STEMS METHANOLIC EXTRACT

Abstract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems extract were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the extract were investigated by using Total Phenolics Content (TPC), Total Flavonoids Content (TFC), DPPH free radical scavenging and b–carotene bleaching assays. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the extract and ascorbic acid were 0.21 and 0.04 mg/mL, respectively. The average percentage of b–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).

Anti-Infectious Plants of the Thai Karen: A Meta-Analysis

“…Tinospora crispa, which had the top CI value in this study, is also used for treatments of infectious diseases in other parts of the world, and its extracts have a broad range of pharmacological activities [37]. For antimicrobial activities, T. crispa includes effects on Staphylococcus aureus, Streptococcus pneumonia, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Saccharomyces cerevisiae [38,39]. The plant produces a variety of secondary metabolites including lactones, steroids, flavonoids, lignan and alkaloids.…”

Study of white frangipani flower and bitter grape stem ethanol extract combination on antibacterial and antioxidant activities

“…T. crispa also is known as Menispermum crispum, M. rumphii and M. tuberculata [9]. The bitter grape stem extract showed antimicrobial and antioxidant activity [10]. *Corresponding Author: candra@faperta.unmul.ac.id A combination of two plant extracts with pharmacological activity may result in a synergistic or antagonistic effect [11], e.g., antioxidant activity [12,13] and antimicrobial activity [14].…”