Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts

Abstract

HighlightsDPPH forms purple color when dissolved in solution and its color is altered to slightly yellowish color with the intervention of rhizome extracts of Curcuma caesia Roxb.at different concentrations showing the scavenging activity against the free radicals generated by DPPH. The scavenging activity of the extracts tested follows the order EECC>MECC>EaECC>AECC.Total phenolic and reducing ability were also found to be increased in ethanolic extract as compared to other extracts.EECC, MECC, AECC were selected to test the antimutagenic activity against Cyclophosphamide (CP) using Salmonella typhimurium strains TA98 and TA100. S9 mix prepared from male mice liver was used to activate the cyclophosphamide. Cyclophosphamide produces free radicals Phosphoramide and Acrolein on activation with S9 mix causing mutations inside the living system which found to be ammeliorated by the secondary metabolites present in plants. All the extracts tested were found to be non mutagenic.CP can also act as positive control for the strain TA98 which was not reported ealier and this can be inhibited by the used of rhizome of Curcuma caesia Roxb. Extract.

Chemical composition and in vitro antioxidant potential of essential oil and rhizome extracts of Curcuma amada Roxb

“…In the radical form, this molecule had an absorbance at 517 nm which disappeared after acceptance of an electron or hydrogen radical from an antioxidant compound to become a stable diamagnetic molecule. The use of DPPH assay provides an easy and a rapid way to evaluate antioxidants by spectrophotometer (Devi, Mazumder, & Devi, 2015). DPPH forms purple colour when dissolved in solution and altered to yellowish colour with interaction of rhizome extract of Curcuma (Pushparani Devi et al, 2015).…”

Anthocyanin content in the black scented rice (Chakhao): its impact on human health and plant defense

“…The determination of the quenching of free radical activity was done using 2,2 -diphenyl-1-picryl hydrazyl (DPPH) described by Devi et al 2015, with some modifications. One ml of 0.1 mM solution of DPPH free radical in methanol was mixed with 1 ml of the extract (50, 100, 150 μg/ml) and after mixing the solutions were incubated for 30 min and then absorbance was measured at 517 nm.…”

Pharmacological activities and medicinal properties of endemic Moroccan medicinal plant Origanum compactum (Benth) and their main compounds

“…It has been accepted that plants and their products represent one of the sources possessing potential chemoprotective properties. Indeed, several secondary metabolites from medicinal plants are shown chemoprotective [79][80][81][82]. O. compactum essential oil and their major compounds are shown to be effective for their mutagenic and antimutagenic effects using the somatic mutation and recombination assay.…”