Antioxidant and antiproliferative activities of phenolics isolated from fruits of Himalayan yellow raspberry (Rubus ellipticus)

Abstract

Yellow Himalayan raspberry, a wild edible fruit, was analyzed for phenolic contents, and antioxidant, antibacterial and antiproliferative activities. Phenolics were extracted using 80 % aqueous solvents containing methanol, acidic methanol, acetone and acidic acetone. Our analysis revealed that the acidic acetone extracts recovered the highest level of total phenolics (899 mg GAE/100 g FW) and flavonoids (433.5 mg CE/100 g FW). Free radical scavenging activities (DPPH, ABTS, superoxide and linoleate hydroperoxide radicals) and ferric reducing activity were highest in the acetone and acidic acetone extracts. No metal chelating or antibacterial activity was detected in any of the extracts. Acetone and methanol extracts showed potent antiproliferative activity against human cervical cancer cells (C33A) with an EC 50 of inhibition at 5.04 and 4. 9 mg/ml fruit concentration respectively, while showing no cytotoxicity to normal PBMCs cells. Therefore, the present study concluded that the yellow Himalayan raspberry is a potent source of phytochemicals having super antioxidant and potent antiproliferative activities.

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

“…As polyphenols and anthocyanins are well-documented antioxidants exhibiting antibacterial action [64,65,66,67,68], it is reasonable to hypothesize that the capacity to inhibit bacterial growth of the investigated wines may be related to the concentration of these compounds.…”

Variation in Chemical Constituents and Antioxidant Activity in Yellow Himalayan (R ubus ellipticus Smith) and Hill Raspberry (R ubus niveus Thunb.)

“…The level of total phenolics and flavonoid content observed in this study was higher than the previously reported values of the species in Garhwal region of Uttarakhand Himalaya (5.50 mg GAE/g fw and 1.79 mg CE/g fw in methanolic extract) (Saini et al . ) and from Western Ghats (0.72 mg GAE/g fw and 0.864 mg QE/g fw) (Karuppusamy et al . ).…”

“…Antioxidant, antibacterial and anti‐proliferative activities of R. ellipticus have already been investigated (Saini et al . ). However, little information is available on antioxidant activity, phenolics and other chemical constituents (Karuppusamy et al .…”

“…; Saini et al . ). The aim of the study was to determine the anthocyanins, carotenoids, ascorbic acid, phenolics content and antioxidant activity of the R. ellipticus and R. niveus and also to investigate plant‐to‐plant variation in these active chemical constituents at the same location of a wild population.…”

Identification of food preservative, stress relief compounds by GC–MS and HR-LC/Q-TOF/MS; evaluation of antioxidant activity of Acalypha indica leaves methanolic extract (in vitro) and polyphenolic fraction (in vivo)

“…DPPH is a stable diamagnetic free radical, dissolved in organic solvents such as methanol and ethanol. Dissolved DPPH radical in solvents visible as intense All values denoted in triplicates Mean ± SD The optical density values are represented with Mean ± SD measurements All Values are triplicates and calculated with Mean ± SD violet colour (oxidised state) and they would be stable by accepting hydrogen or electron followed by proton from donor gives a colourless product (reduced state) (Saini et al 2014). The increase intensity of colourless product decreases the absorbance, whereas, the decrease in colourless intensity increases the absorbance at wavelength 517 nm.…”