ANTIOXIDANT AND CELL PROLIFERATION INDUCTION ACTIVITIES COMBINATION OF HYDROALCOHOL EXTRACT OF Artocarpus lacucha Buch. Ham. LEAVES AND Anredera cordifolia (Ten) Steenis. LEAVES

Abstract

The uncontrolled production of oxygen free radicals and the unbalanced mechanism of antioxidant protection results in the onset of many diseases, such as cancer, diabetes, Alzheimer’s, heart diseases, and aging. Artocarpus lacucha Buch.-Ham. Belongs to the family of Moraceae, popularly regarded as a medicinal plant, and commonly called monkey jack. Anredera cordifolia (Ten.) Steenis also known as Binahong is a family of Basellaceae. Both the plants are widely used in traditional medicine. The aim of this study was to determine the antioxidant activity and proliferation activity of combination ethanol extract of Artocarpus lacucha leaves (EEAL) and Anredera cordifolia leaves (EEAC). The extract was prepared using ethanol 80% with the maceration method. Antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Proliferation activity was determined with an MTT assay. Antioxidant activity from combination with DPPH assay is measured as the percent of scavenging and the best combination is 37.5 µg/mL for EEAL and 12.5 µg/mL for EEAC. Proliferation activity in combination with MTT assay is measured as a percent of viability cells and the best combination is 37.5 µg/mL for EEAL and 37.5 µg/mL for EEAC. The results reveal that combination hydroalcoholic extract of Artocarpus lacucha Buch.-Ham. Leaves and Anredera cordifolia (Ten.) Steenis leaves have antioxidant and proliferative activity

The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. Herbs

“…The flavonoid concentration is expressed as quercetin equivalents in mg per gram of extract. All assays were carried out in triplicate [22,23].…”

“…The IC 50 value is utilized as the parameter for the free radical scavenging test. This is the value achieved as a result of the ability to absorb 50% of free radicals [22]. The DPPH method is used to assess antioxidants because it functions as a synthetic radical that takes hydrogen atoms, transforming DPPH into non-radical molecules [17].…”