Antioxidant and hepatoprotective activities of intracellular polysaccharide from  Pleurotus eryngii  SI-04

Zhang, Chen; Li, Shangshang; Zhang, Jianjun; Hu, Chunlong; Che, Gen; Zhou, Meifang

doi:10.1016/j.ijbiomac.2016.05.104

Cited by 72 publications

(46 citation statements)

References 46 publications

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“…DPPH radical is widely used for assessing the various antioxidants’ scavenging effects, which is a stable free radical molecule. With DPPH radical decreasing, the color of DPPH reaction solution varies from red to yellow even colorless (Zhang et al, ). In this research, the scavenging effects of DIP‐1 and Vc against DPPH radical were presented in Figure b.…”

Section: Resultsmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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“…According to previous reports, the reducing power, prevention of the chain initiation reaction and radical scavenging activity indicate positive antioxidant effects via the interruption of the free radical chain and the simultaneous contribution of a hydrogen atom to Fe 3+ , generating Fe 2+ 27–29 . The scavenging activity towards the DPPH radical, a jarless free radical, is a quick and effective indicator that reflects antioxidant activity when DPPH interacts with electron/hydrogen radicals to form non-radical form (DPPH-H) 30 . As a major type of ROS, the hydroxyl radical damages biomolecules and induces lipid peroxidation, which is not completely eliminated by the human defence system alone 24, 31 .…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective and in vitro antioxidant effects of native depolymerised-exopolysaccharides derived from Termitomyces albuminosus

Zhao

Zhang

et al. 2017

Sci Rep

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In this study, native depolymerised-exopolysaccharides (DEPS) were successfully derived from the exopolysaccharides (EPS) of Termitomyces albuminosus, and its hepatoprotective effects against a high-fat emulsion and in vitro antioxidant activities were investigated. Based on the results of in vitro assays, DEPS showed superior antioxidant activities compared with EPS dose-dependently. According to the in vivo assays both EPS and DEPS significantly decreased the lipid levels, improved the enzymatic activities, and reduced lipid peroxidation in both serum and hepatic homogenates. Furthermore, EPS and DEPS attenuated the high-fat emulsion-induced histopathological injury to the liver. Both EPS and DEPS might be used as natural drugs to treat and protect against hyperlipidaemia and liver injury induced by a high-fat emulsion. In addition, based on the results of GC and HPLC analyses, rhamnose and low molecular weight compounds may play an important role in contributing to the antioxidant activities of EPS and DEPS.

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“…In general, the detected antioxidant activity represents an important added-value, despite the weaker performance of the mycelium and culture medium extracts when compared with the DPPH scavenging activity (EC 50 values ranging from 0.8 to 3 mg/ mL) and reducing power (EC 50 = 0.5 mg/mL) obtained in polysaccharide fractions of P. eryngii (Li & Shah, 2014;Ma et al, 2016;Zhang et al, 2016). However, it should be reminded that the results presented herein were obtained with raw extracts, not with purified fractions.…”

Section: Pleurotus Eryngiimentioning

confidence: 99%

“…The cultured mycelia are becoming a promising alternative as a source of fungal bioactive compounds, mostly due to the shorter incubation time and easier culture conditions (less required space, low probability of contamination and higher production of biomass, when compared to the fruiting bodies) (Gan et al, 2012;Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Wild mushrooms and their mycelia as sources of bioactive compounds: Antioxidant, anti-inflammatory and cytotoxic properties

et al. 2017

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a b s t r a c tMushrooms are important sources of natural bioactive compounds. Pleurotus eryngii (DC.) Quél is recognized for its organoleptic quality and health effects, being extensively commercialized. Instead, Suillus bellinii (Inzenga) Watling is an ectomycorrhizal symbiont, whose main properties were scarcely reported. Considering current trends, the mycelia and the culture media of these mushrooms might be potential sources of bioactive compounds. Accordingly, P. eryngii and S. bellinii were studied for their phenolic acids and sterols, antioxidant capacity, anti-inflammatory effect and anti-proliferative activity. S. bellinii mycelia showed higher contents of ergosterol and phenolic compounds (also higher in its fruiting body) and stronger antioxidant activity than P. eryngii. Conversely, P. eryngii mycelia showed anti-inflammatory (absent in S. bellinii mycelia) and a cytotoxicity similar (sometimes superior) to its fruiting bodies, contrarily to S. bellinii. Furthermore, the assayed species showed differences in the growth rate and produced mycelia, which should be considered in further applications.

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Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04

Cited by 72 publications

References 46 publications

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Hepatoprotective and in vitro antioxidant effects of native depolymerised-exopolysaccharides derived from Termitomyces albuminosus

Wild mushrooms and their mycelia as sources of bioactive compounds: Antioxidant, anti-inflammatory and cytotoxic properties

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