Antioxidant and Hepatoprotective Effect of Penthorum chinense Pursh Extract against t-BHP-Induced Liver Damage in L02 Cells

Hu, Yangyang; Wang, Shengpeng; Wang, Anqi; Lin, Ligen; Chen, Meiwan; Wang, Ying

doi:10.3390/molecules20046443

Cited by 39 publications

(34 citation statements)

References 28 publications

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“…Nevertheless, in modern biological science, there have been only a few reports concerning this useful plant. Some studies have reported on antioxidant and anti-cancer cancer properties of P. chinensis, as well as on potential hepatoprotective, antiviral and anti-diabetic uses (Cao et al 2015;Hu et al 2015;Huang et al 2015;Lu et al 2012;Wang et al 2015;Zhang et al 2013). Certain bioactive compounds including quercitrin, quercetin-3-O-rhamnoside, gallic acid, β-sitosterol, thonningianins A, 5-hydroxy-flavanone-7-Obeta-D-glucoside, 2,4,6-trihydroxybenzoic acid, pinocembrin-7-O-beta-glucoside, 5-methoxy-pinocembrin-7-O-β-D-glucoside and pinocembrin-7-O-[3''-O-galloyl-4'',6''-hexahydroxydiphenoyl]-beta-glucose have been isolated from this plant species (Feng et al 2001;Lu et al 2012;Wang et al 2006Wang et al , 2014.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

Mei

Zhang

2017

Biodiversitas

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Abstract. Mei Z, Khan MA, Zhang X, Fu J. 2017. Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers. Biodiversitas 18: 1243-1249. For the genetic identification and authentication of living organisms, development of Sequence-Characterized Amplified Region (SCAR) markers from Random Amplified Polymorphic DNA (RAPD) fragments is a valuable molecular approach. By using SCAR markers, molecular analysis is simplified to a Polymerase Chain Reaction (PCR) analysis using PCR primers designed from specific sequences of the RAPD amplicons. In this study, RAPD fragments from improved RAPD amplification of a perennial herb Penthorum chinense Pursh from China were cloned into a T-vector, and positive clones were identified by PCR amplification, and sequenced with the Sanger sequencing method for the SCAR marker development. Five SCAR markers were developed that were very specific to P. chinense, and deposited in GenBank (accession numbers: KX671029, KX671030, KX671031, KX671032 and KX671033). BLAST searches of these five nucleotide sequences in the GenBank database showed no identity with markers from other species. This study was developing five specific SCAR markers, has enabled reliable genetic identification of the herbal plant species Penthorum chinense Pursh, useful for authenticating future samples of this important medicinal herb.

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Section: Introductionmentioning

confidence: 99%

Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

Mei

Zhang

2017

Biodiversitas

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“…Although a certain ROS level is essential for cellular survival, cellular death may occur when oxidative stress caused by ROS production, exceeds the cellular antioxidant abilities [14,15]. An excessive ROS production has been reported to cause DNA damage, modify proteins and lipid functions, and activate the related signal pathways [15].…”

Section: Discussionmentioning

confidence: 99%

“…Excessive oxidative stress is reported to be associated with a variety of diseases [14]. Although a certain ROS level is essential for cellular survival, cellular death may occur when oxidative stress caused by ROS production, exceeds the cellular antioxidant abilities [14,15].…”

Section: Discussionmentioning

confidence: 99%

The effects of hydromorphone on astrocytic responses in cerebral ischemia

et al. 2016

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: Ischemic insult during operation could cause ischemic-reperfusion injuries in brain and memory impairments. Total intravenous anesthesia (TIVA) is preferred in brain surgery to promote the use of motor evoked potential monitoring and the use of opioids is common in TIVA. However there were few studies about ischemic protective effect of opioids to astrocytes. Methods: We used astrocytes, which were derived from human brain. We divided groups by conditioning period; i) pre-culture, ii) post-culture, or iii) pre + post-culture. All groups were treated 100 nM hydromorphone. We measured reactive oxygen species (ROS) by flow cytometry with 2',7'-dichloroflurorescin diacetate. Then ROS in astrocytes which treated by opioid receptor antagonist were measured after treating 100 nM hydromorphone. Results: ROS was reduced in hydromorphone treated group, as compared to the control group (only tert-butyl hydroperoxide [TBH] treated). There was no difference in pre-conditioned group and post-conditioned group. However, ROS was much more reduced in pre + post-conditioned group compared to pre-conditioned only or post-conditioned only group. Furthermore each selective -, δ-and κ-opioid receptor antagonists partially negated the effect of hydromorphone. Conclusions: This study provides evidence that hydromorphone has both preconditioning and postconditioning effects on TBHinduced oxidative stress. Furthermore we proved each μ-, δ-and κ-opioid receptor relates to protective mechanism of hydromorphone to astrocytes. (Anesth Pain Med 2016; 11: 23-27)

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“…Generally, the cellular antioxidant defense system counterbalances ROS production to maintain an appropriate balance between oxidants and antioxidants (4). Therefore, antioxidant therapy may be one strategy to prevent cells from excessive exposure to oxidative stress and correct cellular redox homeostasis (5).…”

Section: Introductionmentioning

confidence: 99%

Cytoprotective effects of diosmetin against hydrogen peroxide-induced L02 cell oxidative damage via activation of the Nrf2-ARE signaling pathway

et al. 2018

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Oxidative stress is considered a crucial mediator in the pathogenesis of various liver diseases. The flavone diosmetin has been reported to exhibit antioxidant activities; however, the hepatoprotective effects of diosmetin against oxidative stress, and the underlying molecular mechanisms, remain unknown. The present study aimed to investigate the potential hepatoprotective effects of diosmetin on hydrogen peroxide (H2O2)‑induced oxidative damage in L02 cells and attempted to evaluate the role of the nuclear factor erythroid 2‑related factor 2 (Nrf2)/antioxidant response element pathway in this process. L02 cells were divided into groups: Control (DMSO, diosmetin), H2O2, Trolox or tertiary butylhydroquinone and diosmetin (different doses). Protective effects in L02 cells were determined by CCK‑8, cell apoptosis and lactate dehydrogenase leakage assays. Flow cytometry and inverted fluorescence microscope were used to measure the intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP). Protein expression levels were of Nrf2, heme oxygenase‑1 (HO‑1) and NAD(P)H quinone oxidoreductase‑1 (NQO1) were determined by western blotting and mRNA levels were determined by reverse transcription‑quantitative polymerase chain reaction. The results revealed that H2O2 induced notable injury to L02 cells, as demonstrated by decreased cell viability, increased lactate dehydrogenase release, apoptotic rate and intracellular ROS production, and by the loss of MMP. Conversely, diosmetin (20‑40 µM) significantly reversed the damaging effects of H2O2, which indicated that diosmetin may exhibit potent hepatoprotective potential against H2O2‑induced oxidative damage. Furthermore, pretreatment with diosmetin elevated mRNA and protein expression levels of Nrf2, HO‑1 and NQO1. The present study is the first, to the best of our knowledge, to demonstrate that activation of the Nrf2/NQO1‑HO‑1 signaling pathway maybe involved in the cytoprotective effects of diosmetin against oxidative stress. Therefore, diosmetin may be considered a promising therapeutic agent for the treatment of various liver diseases associated with oxidative stress.

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Antioxidant and Hepatoprotective Effect of Penthorum chinense Pursh Extract against t-BHP-Induced Liver Damage in L02 Cells

Cited by 39 publications

References 28 publications

Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

Short Communication: Rapid and accurate genetic authentication of Penthorum chinense by improved RAPD-derived species-specific SCAR markers

The effects of hydromorphone on astrocytic responses in cerebral ischemia

Cytoprotective effects of diosmetin against hydrogen peroxide-induced L02 cell oxidative damage via activation of the Nrf2-ARE signaling pathway

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