Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Omoruyi, Beauty E; Bradley, Graeme; Afolayan, Anthony Jide

doi:10.1186/1472-6882-12-215

Cited by 96 publications

(83 citation statements)

References 31 publications

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“…The results suggest that A. cobbe leaf extract might serve as a potent therapeutic agent for scavenging of free radicals and the regulation of pathological conditions caused by oxidative stress. The observed antioxidant effect of the plant extract could be attributed to the presence of polyphenolic compounds (Omoruyi et al, 2012). The results are in line with the previous studies which reported that the free radical scavenging activity of plant extracts correlate with the phenolic content (Omoruyi et al, 2012;Jain et al, 2014).…”

Section: Discussionsupporting

confidence: 81%

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

Jain

Hossain²,

Fatema³

et al. 2014

American Journal of Pharmacology and Toxicology

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Allophylus cobbe L. Raeuschel (Family-Sapindaceae) is a medicinal plant used traditionally for the treatment of various health risks like pain, inflammation, ulcers and wounds in Bangladesh. This study determined the polyphenolic compounds and evaluated the analgesic, anti-inflammatory and antioxidant effects of the ethanol extract of Allophylus cobbe leaves. High Performance Liquid Chromatography (HPLC) analysis was used to determine the polyphenolic compounds present in the extract. The analgesic activity was evaluated by hot plate and acetic acid induced writhing in mice at two different doses of 250 and 500 mg kg −1 body weight. The extract was also investigated for the anti-inflammatory effect on rats at above mentioned doses using carrageenan induced rat paw edema method. The antioxidant potential of the extract was determined in terms of radical scavenging ability of the stable 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH) free radical. Preliminary phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins and glycosides in the extract. High Performance Liquid Chromatography (HPLC) analysis also confirmed the presence of polyphenolic compounds such as (+)-catechin hydrate, (-)-epicatechin, caffeic acid, p-coumaric acid and quercetin. The extract increased the licking time of hind paw when placed on a hot plate by 35.31% at 250 mg kg −1 dose which is comparable to the increase shown by diclofenac sodium (42.73%) at the 3rd h of study in hot plate test. Moreover, the extract also showed good analgesic effect in acetic acid induced writhing test. The percent inhibition of writhing response by the extract was 85.96 and 78.07% at 250 and 500 mg kg −1 doses respectively while that of the standard drug was 66.67%. Furthermore, the extract also reduced carrageenan induced paw edema formation; the most prominent inhibition was found to be 58.88% (250 mg kg −1 ) at the 3rd h of study. The DPPH radical scavenging activity of the extract increased markedly with increasing concentrations. At a concentration of 200 µg mL −1 , the scavenging activity of the ethanol extract (91.53% inhibition) was comparable to that of the standard ascorbic acid (99.3% inhibition). Our results suggest that Allophylus cobbe extract possesses significant antinociceptive, anti-inflammatory and free radical scavenging activities which may justify the folkloric use of the plant in several communities for conditions such as colic, fever, inflammatory rheumatic pains and other oxidative stress associated disorders.

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Section: Discussionsupporting

confidence: 81%

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

Jain

Hossain²,

Fatema³

et al. 2014

American Journal of Pharmacology and Toxicology

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“…At the concentration 0.05 there is no significant different between the aqueous extract and the standard (P> 0.05). The IC50 value of the acetone leaf extract (0.13, 61.7%) is significantly higher than that of standard (vitamin C 0.30, 74.3%) the result is in agreement with Omoruyi et al (2012), who reported that IC50 values of methanol plant extract is significantly higher than that of BHT and Rutin. It was also revealed that the leaf extracts contain antioxidant properties than the bark extracts as shown in Table 2; this implies that most bioactive compound are stored on the trichomes which might probably be on the leaves as reported by Afolayan and Meyer (1997).…”

Section: Abts Radical -Scavenging Activitysupporting

confidence: 82%

“…However, there is no significance difference in the percentage inhibition of vitamin C and the acetone extract. The minimum inhibitory concentration obtained to reduce the nitric oxide radicals by 50% of the extracts of the leaves and bark of C. anisata is in the following order: acetone bark > dichloromethane bark > aqueous leaf > dichloromethane leaf > aqueous bark, it can be deduced that organic solvent processes strong antioxidant activity, this study is in agreement with other researchers investigating scavenging activity of nitric oxide (Omoruyi et al, 2012, Otang et al, 2012). This will contribute to scientific knowledge and could be a lead to potent herbal drug to the ailments and diseases associated to oxidative stress.…”

Section: Nitric Oxide Scavenging Activitysupporting

confidence: 81%

“…The saponin content in the plant extracts was estimated as previously described (Jimoh et al, 2010, Omoruyi et al, 2012. Ten grams of the powdered sample was placed in 200 ml of 20% ethanol.…”

Section: Saponin Determinationmentioning

confidence: 99%

“…(Ewa et al, 2009) The antioxidant properties of polyphenol compounds rely on their capability in scavenging free radicals and its potential to reduce iron (Nihal et al, 2007). Solvent of extraction determine the amount of total polyphenol in plants and its antioxidant activities (Omoruyi et al, 2012). It has been reported that acetone extracted both polar and non-polar compounds from plants (Wintola and Afolayan, 2011), another report also confirmed that the acetone extract total phenols in olive oil when compared to other solvent extract (Brenes et al, 2000) In India, hexane, ethyl acetate/ chloroform, alcohol, and acetone root extract of Clausena anisata have been reported for its scavenging activities and the presence of some secondary metabolite such as, alkaloid, protein-carbohydrate and amino acid (Tarnam et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical and antioxidant investigations of a Clausena anisata hook, a South African medicinal plant

Lawal¹,

Grierson²,

Afolayan³

2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Clausena anisata (Willd). Hook (Rutaceae), also known as Iperepesi in Xhosa language is a medicinal plant widely used by herbalists for the treatment and/or management of several ailments such as chronic cough, tuberculosis and lung ulceration in Eastern Cape, South Africa. With reference to the information gathered in our previous study, we investigated the plant's phyto-constituents, as well as its inhibitory effects using aqueous and two different organic solvent of extractions in order to justify its folkloric usage. Methods: Antioxidant activity of the plant was screened through 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt, nitric oxide (NO), and ferric reducing power. Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were investigated using spectroscopic techniques. Results: There were no significant differences in the flavonoid and proanthocyanidins contents between the leaves and bark extracts of C. anisata respectively, while the total phenolic content of the bark extract of C. anisata was significantly higher than that of the C. anisata leaf. The acetone extracts of both the leaf and bark indicated strong antioxidant activities. Conclusion:The observed activities of the plant extracts could be attributed to the high contents of the phenolics, alkaloids, flavonoids, saponins, proanthocyanidins and tannin. The acetone extracts of the plants have also exhibited strong antioxidant activities in vitro. It has been established scientifically that oxidative stress is linked with several degenerative conditions and diseases; the inhibitory effects of these plant extracts on the free radicals could logically justify the folkloric usage of C. anisata leaf and bark in the Eastern Cape for the treatment of respiratory infection diseases.

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In vitro antidiabetic, antioxidant, antimicrobial, and cytotoxic activity of Murraya koenigii leaf extract intercedes ZnO nanoparticles

Sharma

Nagraik

Venkidasamy³

et al. 2022

Luminescence

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Nanotechnology is an emerging field with tremendous potential and usage of medicinal plants and green preparation of nanoparticles (NPs) is one of the widely explored areas. These have been shown to be effective against different biological activities such as diabetes mellitus, cancer, antioxidant, antimicrobial, etc. The current studies focus on the green synthesis of zinc NPs (ZnO NPs) from aqueous leaf extract of Murraya koenigii (MK). The synthesized Murraya koeingii zinc oxide NPs (MK ZnO NPs) were characterized using UV-visible spectroscopy, dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy-dispersive spectrum (EDS) and cyclic voltammetry (CV). The synthesized MK ZnO NPs were evaluated for their in vitro antidiabetic, antioxidant, antimicrobial, and cytotoxic activity. They demonstrated significant antidiabetic and cytotoxic activity, as well as moderate free-radical scavenging and antibacterial activity.antibacterial and cytotoxic activity, antidiabetic activity, green synthesis, Murraya koenigii, zinc oxide nanoparticles | INTRODUCTIONM. koenigii (MK) also called curry leaf in the Indian family, is indigenous to Sri Lanka and other South Asian countries and belongs to the Rutaceae family, which includes over 150 genera and 1600 species. [1] MK plant is used for its antioxidative, antimicrobial, cytotoxic, antiulcer, positive inotropic, and cholesterol reducing activities. [2] Several compounds have been isolated from different parts. Mathur et al.(2011) confirmed the antifungal activity of some components of M. koenigii. [3] The apoptotic activity of maharini, pravadoline-D, and morpholine-I, carbazole alkaloids of M. koenigii showed effective results against the human myeloid cancer cell line HL-60. [4] Leaf extract was found positively to induce apoptosis in human myeloid cancer cell lines. [5] Mahanimbine, a phytocompound showed antidiabetic activity. By increasing the pancreatic secretion of insulin from beta-cells and by increasing the uptake of glucose, mahanimbine decreases blood sugar levels. Leaves have been shown to increase glycogenesis, while decreasing glycogenolysis and gluconeogenesis. [6] Diabetes mellitus (DM) is an endocrine metabolic disorder that shows elevated blood glucose levels due to an insufficient amounts of insulin [7,8] Currently, the overall global prevalence of diabetes for all age groups was estimated to be 2.0% (124 million people) in 1997, [9] 2.8% (171 million people) in 2000, 3.6% (221 million people) in 2010, and 4.4% (366 million people) in 2030 and will be 700.2 million people by 2045 (International Diabetes Federation, 2020). [9,10] Compared with developed countries, DM seems to be more prevalent in developing countries. As a result, diabetes has emerged as one of the most serious health threats, with a significant socioeconomic effect.The pharmacological interventions for the treatment of diabetes include the oral hypoglycaemic agents and insulin therapy. When

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Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province

Cited by 96 publications

References 31 publications

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

Anti-Inflammatory, Analgesic and Antioxidant Activities of <i>Allophylus Cobbe</i> Leaves

Phytochemical and antioxidant investigations of a Clausena anisata hook, a South African medicinal plant

In vitro antidiabetic, antioxidant, antimicrobial, and cytotoxic activity of Murraya koenigii leaf extract intercedes ZnO nanoparticles

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